Effects of resveratrol on methotrexate-induced intestinal injury

Abstract

BACKGROUND: Methotrexate (MTX) is an anticancer drug. Many studies have reported that MTX causes oxidative stress-associated damage in the small intestine. The purpose of this study was to investigate the possible protective effect of resveratrol (RES), an antioxidant, against MTX-induced damage in the small intestine. MATERIALS AND METHODS: Twenty-four Spraque Dawley rats were randomly divided into four groups; the control group, the RES group given 20 mg/kg RES for 10 days, the MTX group given single dose 30 mg/kg MTX, MTX+RES group given 20 mg/kg RES i.p. for 7 days and 30 mg/ kg MTX i.p. on the 7th day, RES being maintained for 3 further days. All rats were sacrifi ced on the 10th day, and small intestinal tissue was removed for histopathological and biochemical analysis. Additionally, mucosal apoptosis was analyzed using the TUNEL method. RESULTS: Histopathologically, villar fusion, atrophic villus epithelium, cystic expansion in crypts, hemorrhage and infl ammatory cell infi ltration were seen in the small intestine in the MTX group. In the MTX+RES group this histopathological damage decreased signifi cantly. Apoptotic score was signifi cantly higher in the MTX group and signifi cantly lower in the MTX+RES group. Tissue malondialdehyde (MDA) level was signifi cantly higher in the MTX group. Superoxide dismutase (SOD) activity was signifi cantly decreased in the MTX group. The MDA level in the MTX+RES group decreased while SOD and catalase (CAT) activities rose, this was not statistically signifi cant. CONCLUSİONS: RES treatment may ameliorate MTX induced small intestine damage especially at histopathological level (Tab. 2, Fig. 2, Ref. 41).

Resveratrol and inflammatory bowel disease

“…Treatment with resveratrol ameliorated methotrexate‐induced intestinal injury (it was associated with lower histopathological and apoptotic scores). It also decreased tissue MDA levels and enhanced SOD and CAT activities and total glutathione (GSH) in vivo . A recent report showed that resveratrol was more effective than 5‐aminosalicylic acid and could activate the nuclear factor erythroid‐2 and PPAR‐γ pathways in cytokine‐stimulated human intestinal cells, both of which are considered new therapeutic targets in IBD .…”

Resveratrol Attenuates Heat Stress-Induced Impairment of Meat Quality in Broilers by Regulating the Nrf2 Signaling Pathway

“…RES, a natural plant polyphenol, has been proven to exhibit outstanding antioxidant function. Yulug et al [ 36 ] reported that adding RES to the diet of mice could reduce MDA content and increase the activities of SOD and CAT. Liu et al [ 9 ] found that adding RES to the diet of black-bone chickens under HS could increase SOD, GSH-Px, and CAT activity and reduce MDA content in serum.…”

Resveratrol Siçanlarda Oksi̇dati̇f Stresi̇ Ve Apoptozu Baskilayarak Metotreksat Kaynakli Yumurtalik Hasarini Hafi̇fleti̇r

“…Many studies have examined the use of RES as a therapeutic agent to treat various pathological and metabolic disorders (15). In previous studies, the protective effects of resveratrol on MTX-induced liver damage (29), intestinal damage (30,31) and ileum damage (32) has been reported.…”