Effects of Residual Oil Fly Ash (ROFA) in Mice with Chronic Allergic Pulmonary Inflammation

Arantes-Costa, Fernanda Magalhães; Lopes, Fernanda Degobbi Tenório Quirino dos Santos; Toledo, Alessandra Choqueta de; Magliarelli-Filho, Pedro A.; Moriya, Henrique Takachi; Carvalho-Oliveira, Regiani; Mauad, Thaís; Saldiva, Paulo Hilário Nascimento; Martins, Mílton A.

doi:10.1177/0192623308317427

Cited by 50 publications

(55 citation statements)

References 52 publications

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“…In addition, alveolar septa were thickened and contained increased numbers of macrophages and eosinophils. Similar changes in lung architecture and lung cell and inflammatory responses previously have been observed after exposure to residual oil fly ash particles [37] and urban air particulate matter [38] in an allergic pulmonary inflammation model.…”

Section: Resultssupporting

confidence: 74%

Adjuvant effect of zymosan after pulmonary treatment in a mouse ovalbumin allergy model

Young

Wolfarth

Roberts

et al. 2013

Experimental Lung Research

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An association has been observed between indoor mold contamination and lung allergy and asthma. This relationship is not fully understood. 1→3-β-Glucan is the major cell wall component of fungi and a good marker of fungi exposure. The objective was to evaluate the adjuvant effect of zymosan, a crude yeast cell wall preparation of 1→3-β-glucan, during ovalbumin (OVA) sensitization in an allergy model. BALB/c mice were sensitized by pharyngeal aspiration with saline, 50 µg of OVA, or OVA with 1, 10, 50, or 75 µg of zymosan on days 0, 7, and 14. One week after sensitization, each sensitized animal group was challenged with an aspiration dose of 50 µg of OVA once a week for 2 weeks. At 1 day after the last aspiration, bronchoalveolar lavage fluid and blood was collected, and markers of lung allergy and inflammation were assessed. An adjuvant effect of zymosan on OVA allergy during sensitization was observed as indicated by significant elevations in lung eosinophils, serum OVA-specific IgE, and lung IL-5 in the groups sensitized with zymosan and OVA. Pulmonary treatment with zymosan also amplified lung inflammation. Elevations were observed in lung neutrophils, TNF-α, and parameters of lung injury in the groups primed with both zymosan and OVA. In nearly all parameters, a non-linear dose-response relationship was observed in the groups primed with OVA and zymosan. The optimum adjuvant dose of zymosan was 10 µg. This study demonstrated an adjuvant effect of zymosan when exposures occurred during the sensitization phase in an OVA-induced allergy model in BALB/c mice.

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Section: Resultssupporting

confidence: 74%

Adjuvant effect of zymosan after pulmonary treatment in a mouse ovalbumin allergy model

Young

Wolfarth

Roberts

et al. 2013

Experimental Lung Research

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“…Ambient PM2.5 in 25 l PBS (1.6 g/g body wt) or 25 l sterile PBS (control) were intranasally delivered to 3-mo-old male mice over a 5-min period twice for 5 days (3,47). PM2.5 particles were collected from the filters installed in the "OASIS-1" system during the "real-world" PM2.5 exposure period.…”

Section: Methodsmentioning

confidence: 99%

Airborne particulate matter selectively activates endoplasmic reticulum stress response in the lung and liver tissues

Laing¹,

Wang²,

Briazova³

et al. 2010

American Journal of Physiology-Cell Physiology

188

145

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“…Control and AT groups were exposed to saline solution. Lung histology 5-mm thick sections of the lungs were stained with haematoxylin and eosin to analyse the lung architecture and quantification of airway smooth muscle thickness [14,20]. Picrosirius was used for bronchial collagen fibre quantification [14,21], Weigert's resorcin fuchsin with oxidation was used for elastic fibres [22] eosinophils [14,23].…”

Section: Protocol Of Chronic Allergic Lung Inflammationmentioning

confidence: 99%

Aerobic training reverses airway inflammation and remodelling in an asthma murine model

Silva¹,

Vieira²,

Duarte³

et al. 2009

European Respiratory Journal

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Aerobic training (AT) decreases dyspnoea and exercise-induced bronchospasm, and improves aerobic capacity and quality of life; however, the mechanisms for such benefits remain poorly understood. The aim of the present study was to evaluate the AT effects in a chronic model of allergic lung inflammation in mice after the establishment of airway inflammation and remodelling.Mice were divided into the control group, AT group, ovalbumin (OVA) group or OVA+AT group and exposed to saline or OVA. AT was started on day 28 for 60 min five times per week for 4 weeks. Respiratory mechanics, specific immunoglobulin (Ig)E and IgG 1 , collagen and elastic fibres deposition, smooth muscle thickness, epithelial mucus, and peribronchial density of eosinophils, CD3+ and CD4+, IL-4, IL-5, IL-13, interferon-c, IL-2, IL-1ra, IL-10, nuclear factor (NF)-kB and Foxp3 were evaluated.The OVA group showed an increase in IgE and IgG 1 , eosinophils, CD3+, CD4+, IL-4, IL-5, IL-13, NF-kB, collagen and elastic, mucus synthesis, smooth muscle thickness and lung tissue resistance and elastance. The OVA+AT group demonstrated an increase of IgE and IgG 1 , and reduction of eosinophils, CD3+, CD4+, IL-4, IL-5, IL-13, NF-kB, airway remodelling, mucus synthesis, smooth muscle thickness and tissue resistance and elastance compared with the OVA group (p,0.05). The OVA+AT group also showed an increase in IL-10 and IL-1ra (p,0.05), independently of Foxp3.AT reversed airway inflammation and remodelling and T-helper cell 2 response, and improved respiratory mechanics. These results seem to occur due to an increase in the expression of IL-10 and IL-1ra and a decrease of NF-kB.

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Effects of Residual Oil Fly Ash (ROFA) in Mice with Chronic Allergic Pulmonary Inflammation

Cited by 50 publications

References 52 publications

Adjuvant effect of zymosan after pulmonary treatment in a mouse ovalbumin allergy model

Adjuvant effect of zymosan after pulmonary treatment in a mouse ovalbumin allergy model

Airborne particulate matter selectively activates endoplasmic reticulum stress response in the lung and liver tissues

Aerobic training reverses airway inflammation and remodelling in an asthma murine model

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