Effects of repeated silver nanoparticles exposure on the histological structure and mucins of nasal respiratory mucosa in rats

Hyun, Jin-Sook; Lee, Byoung‐Seok; Ryu, Hyeon Yeol; Sung, Jae Hyuck; Chung, Kyu Hyuck; Yu, Il Je

doi:10.1016/j.toxlet.2008.08.003

Cited by 101 publications

(62 citation statements)

References 29 publications

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“…Many researchers suggested that nanoparticles will cause inflammatory diseases including pneumoconiosis, emphysema, and mesothelioma by disturbing homeostasis of body (Hussain et al, 1994;Lam et al, 2004;Shvedova et al, 2005;Chen et al, 2006;Chou et al, 2008). According to the previous publications, any significant toxicological change during the 28-day inhalation of AgNPs was not shown in rats (Hyun et al, 2008). Lung and liver were the major target tissues for prolonged AgNPs exposure, and no observable adverse effect level (NOAEL) of AgNPs was determined as 100 µg/m3 (Sung et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Induction of inflammatory responses and gene expression by intratracheal instillation of silver nanoparticles in mice

2011

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The adverse effects of silver nanoparticles (AgNPs) to human and environment have not been known well, although AgNPs are now widely applied to consumer products. In this study, we investigated the inflammatory responses including cytokine production and gene expression in mice after a single intratracheal instillation of AgNPs. As results, pro-inflammatory cytokines (IL-1, TNF-a, and IL-6) and Th0 cytokine (IL-2) were progressively increased by the day 28 after a single instillation. The secretion of Th2 type cytokine was more dominant than that of Th1 type cytokine, and the increase of B cell distribution was also observed. But, histopathological changes in lung were observed only at day 1 after instillation. We identified the changes of gene expression induced by AgNPs in lung tissue using microarray. The 261 genes related to inflammation and tissue damages including Saa3, Krt 13, Lor, Krtdap, and Lcn 2 were up-regulated by over 2 fold, while 103 genes including H2-Ea, Chka, BC030476, Heg1 and Hbb-b1 were down-regulated. Based on the data, it is suggested that AgNPs may induce Th2 type dominant inflammatory responses and tissue damage in the lung of mice.

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Section: Discussionmentioning

confidence: 99%

Induction of inflammatory responses and gene expression by intratracheal instillation of silver nanoparticles in mice

2011

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“…Exposure to AgNPs can occur through inhalation, dermal contact, or ingestion. According to a previous publication that used an in vivo model, there were no significant toxicological changes during 28 days of AgNP inhalation in rats (Hyun et al, 2008). The lungs and liver were the major target tissues for prolonged AgNP exposure through inhalation, and the no observable adverse effect level (NOAEL) of AgNPs was determined to be 100 µg/m 3 (Sung et al, 2009).…”

Section: Introductionmentioning

confidence: 97%

Bioavailability and Toxicokinetics of citrate-coated silver nanoparticles in rats

et al. 2011

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Bioavailability, tissue distribution, blood concentration, and excretion of citrate-coated silver nanoparticles (AgNPs; size, 7.9 ± 0.95 nm by TEM diameter) were investigated. Male SD rats were treated by a single oral or intravenous administration of either 1 or 10 mg/kg AgNPs. Silver concentration of blood was determined at 10 min, and at 1, 2, 4, 8, 24, 48, and 96 h after treatment. Silver in the liver, lungs, and kidneys was also measured at 24 and 96 h after treatment. Excretion of silver nanoparticles via feces and urine was determined at 24 h after treatment. After oral administration, most AgNPs were found in feces, and their blood concentration was very low. This suggests that absorption through the gastrointestinal tract was not good. However, a high level of silver in the blood was detected after tail vein injection. When rats were injected with 1 mg/kg AgNPs, the silver concentration of blood was significantly elevated at 10 min after injection; the level subsequently decreased. In the rats treated with 10 mg/kg AgNPs, the elevated level did not decrease, but was maintained during the experimental period. On the basis of the values of AUC(oral)/AUC(iv), the bioavailability of orally administered AgNPs was 1.2% in the group treated with 1 mg/kg AgNPs and 4.2% in the group treated with 10 mg/kg AgNPs. AgNPs accumulated in the liver, lungs, and kidneys; the accumulated AgNPs were released into the blood stream. AgNP levels in the urine were extremely low compared to the levels in the feces. When rats were injected with AgNPs, these particles were also detected in feces at 24 h after treatment, which suggests bile secretion of AgNPs.

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“…19 Furthermore, AgNPs can induce inflammatory responses such as inflammatory cell infiltration and chronic alveolar inflammation, and can further induce the impairment and dysfunction of brain cells and immunotoxicity. 20,21 AgNPs were also found to induce hepatotoxicity, in which the liver exhibited lymphocytic infiltration, and promoted the expression of genes associated with apoptosis and inflammation. 22 In vitro studies showed that AgNPs can damage the mitochondria, and increase reactive oxygen species production and apoptosis in a p53-dependent process involving reactive oxygen species and the c-Jun N-terminal kinase cascade, or via the IKK/NF-κB pathway.…”

Section: Introductionmentioning

confidence: 99%

Effects of silver nanoparticles on neonatal testis development in mice

Zhang¹,

Gurunathan²,

Kim³

2015

IJN

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Background Metal nanoparticles (MNPs) play an important role in consumer products. An increasing use of MNPs has raised concerns about potential risks for human health. Therefore, in vivo tests of MNPs are urgently required. Using mice as a model animal, the aim of the present study was designed to investigate the effect of biologically synthesized silver nanoparticles (AgNPs) on spermatogenesis in neonatal mice. Methods AgNPs were synthesized using Bacillus funiculus . The prepared nanoparticles were characterized using various analytical techniques such as UV–visible spectroscopy, X-ray diffraction, Fourier transform-infrared spectroscopy, and transmission electron microscopy. The prepared AgNPs were used to investigate testis development in neonatal mice. Institute of Cancer Research neonatal male mice were used in all experiments and were treated with different doses (0, 1, and 5 mg/kg) of AgNPs five times (interval of 3 days from postnatal day [PND] 8–21) by abdominal subcutaneous injection. Results The results showed that the sperm abnormalities such as quality and quantity were significantly increased by the synthesized AgNPs. The diameter of the convoluted tubules shrank significantly in mice treated with AgNPs on PND28 and PND42. The results of reverse transcription-quantitative polymerase chain reaction indicated that the E1f1ay , Gsta4 , and Fdx1 genes were up-regulated, and the Amh , Cx43 , and Claudin-11 genes were down-regulated in response to AgNPs exposure on PND28; however, these genes recovered at PND60. AgNPs had no effect on the recombination levels of chromosomes in germ cells. Conclusion These results demonstrated the adverse effects of AgNPs on the male reproductive tract, particularly spermatogenesis and the quality of sperm. This study suggests that the development of nanomaterials should be safer and non-toxic to the living organisms and the potential reprotoxicity of AgNPs should be investigated more carefully.

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Effects of repeated silver nanoparticles exposure on the histological structure and mucins of nasal respiratory mucosa in rats

Cited by 101 publications

References 29 publications

Induction of inflammatory responses and gene expression by intratracheal instillation of silver nanoparticles in mice

Induction of inflammatory responses and gene expression by intratracheal instillation of silver nanoparticles in mice

Bioavailability and Toxicokinetics of citrate-coated silver nanoparticles in rats

Effects of silver nanoparticles on neonatal testis development in mice

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