Effects of repeated electroejaculations on kinematic sperm subpopulations and quality markers of Mexican creole goats

Vázquez, Amparo; Cedillo, Morón; Quezada, V.J.; Rivas, Alejandro; Salinas, Elizabeth Morales; Ayala, E. M. E.; Hernández, Medrano; González, Raquel; Martínez, Andrés

doi:10.1016/j.anireprosci.2014.12.009

Cited by 13 publications

(10 citation statements)

References 29 publications

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“…However, a well-grounded statistical model to determine the number of clusters (subpopulations) to be considered is not yet available for CASA data (Martínez-Pastor et al 2011). Most of these studies considered variations in the frequencies of determined motile subpopulations to assess differences among individual or repeated ejaculates (Vázquez et al 2015;Kanno et al 2017), possible fertility of ejaculates (Quintero-Moreno et al 2007;García-Á lvarez et al 2014) and the effect of short-term storage and cryopreservation on sperm quality (Martinez-Pastor et al 2005;Núñez-Martínez et al 2006;Dorado et al 2011).…”

Section: Motility Subpopulationsmentioning

confidence: 99%

Current perspectives of CASA applications in diverse mammalian spermatozoa

Horst

Maree

Plessis

2018

Reprod. Fertil. Dev.

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Since the advent of computer-aided sperm analysis (CASA) some four decades ago, advances in computer technology and software algorithms have helped establish it as a research and diagnostic instrument for the analysis of spermatozoa. Despite mammalian spermatozoa being the most diverse cell type known, CASA is a great tool that has the capacity to provide rapid, reliable and objective quantitative assessment of sperm quality. This paper provides contemporary research findings illustrating the scientific and commercial applications of CASA and its ability to evaluate diverse mammalian spermatozoa (human, primates, rodents, domestic mammals, wildlife species) at both structural and functional levels. The potential of CASA to quantitatively measure essential aspects related to sperm subpopulations, hyperactivation, morphology and morphometry is also demonstrated. Furthermore, applications of CASA are provided for improved mammalian sperm quality assessment, evaluation of sperm functionality and the effect of different chemical substances or pathologies on sperm fertilising ability. It is clear that CASA has evolved significantly and is currently superior to many manual techniques in the research and clinical setting.

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Section: Motility Subpopulationsmentioning

confidence: 99%

Current perspectives of CASA applications in diverse mammalian spermatozoa

Horst

Maree

Plessis

2018

Reprod. Fertil. Dev.

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“…Motile sperm subpopulations exists in any semen sample . Sexual steroids are important regulators of reproduction at distinct levels, but their effects on sperm subpopulations have not been fully explored .…”

Section: Discussionmentioning

confidence: 99%

“…The existence of sperm cell subpopulations in semen is widely accepted , and is more evident when multiple parameters are evaluated. The use of a CASA (computer‐assisted sperm analysis) system permits the objective evaluation of multiple parameters of motility.…”

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confidence: 99%

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Effect of sexual steroids on boar kinematic sperm subpopulations

Ayala¹,

Martínez

2017

Cytometry Pt A

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Here, we show the effects of sexual steroids, progesterone, testosterone, or estradiol on motility parameters of boar sperm. Sixteen commercial seminal doses, four each of four adult boars, were analyzed using computer assisted sperm analysis (CASA). Mean values of motility parameters were analyzed by bivariate and multivariate statistics. Principal component analysis (PCA), followed by hierarchical clustering, was applied on data of motility parameters, provided automatically as intervals by the CASA system. Effects of sexual steroids were described in the kinematic subpopulations identified from multivariate statistics. Mean values of motility parameters were not significantly changed after addition of sexual steroids. Multivariate graphics showed that sperm subpopulations were not sensitive to the addition of either testosterone or estradiol, but sperm subpopulations responsive to progesterone were found. Distribution of motility parameters were wide in controls but sharpened at distinct concentrations of progesterone. We conclude that kinematic sperm subpopulations responsive to progesterone are present in boar semen, and these subpopulations are masked in evaluations of mean values of motility parameters. © 2017 International Society for Advancement of Cytometry.

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“…Another aspect is that the correct determination of sperm tracks results in a fundamental shift in the determination of motility and morphology subpopulation structure. The fact that an ejaculate is constituted of different subpopulations of spermatozoa has been well established in a great variety of species (boar (Abaigar, Holt, Harrison, & del Barrio, ; Flores et al., ; Holt, Holt, & Moore, ; Soler et al., ), bull (Valverde et al., ; Yániz et al., ), donkey (Flores et al., ), eel (Gallego et al., ), fox (Soler et al., , ), gazelle (Abaigar et al., ), goat (Vázquez et al., ), ram (Luna et al., ), stallion (Ortega‐Ferrusola et al., ), human (Vásquez, Soler, Camps, Valverde, & García‐Molina, ; Yániz et al., ), salmon (Caldeira et al., )), but most of these studies were not designed for considering the effect of the FR.…”

Section: Introductionmentioning

confidence: 99%

Effect of frame rate capture frequency on sperm kinematic parameters and subpopulation structure definition in boars, analysed with aCASA‐Mot system

Valverde

Madrigal

Caldeira

et al. 2018

Reprod Domestic Animals

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Motility is the most widely used indicator of sperm quality. Computer-Assisted Semen Analysis (CASA) allows the objective evaluation of sperm motility parameters. CASA technology is a common tool to predict semen doses in farm animal reproduction. The kinds of video cameras used until now for image acquisition have presented limited frame rates (FR), which have a negative influence on the quality of the obtained data. The aim of the present work was to define the optimal frame rate for a correct evaluation of boar sperm motility and its subpopulation structure. Eighteen ejaculates from nine mature boars of the Pietrain breed were used. Using the ISAS v1 CASA-Mot system, with a video camera working up to 200 Hz, six FRs (25, 50, 75, 100, 150 and 200 fps) were compared. ISAS D4C20 counting chambers, warmed to 37°C, were used. FR affected all the kinematic parameters, with curvilinear velocity (VCL) and BCF the most sensitive ones. All the parameters showed differences among animals. Non-linear correlation showed the asymptotic level for VCL at 212 fps, being the highest FR for all the parameters. For future studies based just on progressive motility, almost 100 fps FR for 0.5 s must be used, while when kinematics must be considered, almost 212 fps for one-second should be analysed. Three principal components were obtained (velocity, progressivity and oscillation), being similar at 50 and 200 fps. Cells were grouped in four subpopulations but with different kinematic and cellular distribution at both FRs.

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Effects of repeated electroejaculations on kinematic sperm subpopulations and quality markers of Mexican creole goats

Cited by 13 publications

References 29 publications

Current perspectives of CASA applications in diverse mammalian spermatozoa

Current perspectives of CASA applications in diverse mammalian spermatozoa

Effect of sexual steroids on boar kinematic sperm subpopulations

Effect of frame rate capture frequency on sperm kinematic parameters and subpopulation structure definition in boars, analysed with aCASA‐Mot system

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