Effects of relaxin on the fertilization capacity of human spermatozoa

Park, Joo Myun; Ewing, Keith L.; Miller, F.

doi:10.1016/0020-7292(89)90505-5

Cited by 5 publications

(6 citation statements)

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“…Also, immunoassayable relaxin detected in boar seminal plasma is positively correlated with sperm motility characteristics (Juang et al ., 1990; Sasaki et al ., 2001), although a similar correlation was not found in humans (Brenner et al ., 1987; Schieferstein et al ., 1989). Furthermore, incubation of mouse spermatozoa with porcine relaxin increases the rate of in vitro fertilization of mouse oocytes (Pupula et al ., 1986) and porcine relaxin enhances the penetrative capacity of human spermatozoa in the zona‐free hamster egg penetration test (Park et al ., 1988). In addition, 125 I‐labelled human relaxin was reported to bind to human spermatozoa with a high affinity (Carrell et al ., 1995), and we have successfully identified specific relaxin binding sites on boar spermatozoa, and have shown that relaxin binds mainly to the acrosome region along with some binding to the mid‐piece and tail (Kohsaka et al ., 2001).…”

Section: Discussionmentioning

confidence: 99%

Seminal immunoreactive relaxin in domestic animals and its relationship to sperm motility as a possible index for predicting the fertilizing ability of sires

et al. 2003

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Although immunoassayable relaxin has been detected in human and boar seminal plasma, there is no evidence suggesting the existence of immunoreactive relaxin in the seminal plasma of other domestic animals. The first objective of this study was to determine whether immunoreactive relaxin was present in the seminal plasma of bulls, rams and he-goats. In addition, the correlation of immunoreactive relaxin with sperm motility as an index for predicting the fertilizing ability of bull sires was investigated. Semen with normal sperm motility was collected from bulls, rams and he-goats, and the relaxin immunoreactivity of the semen samples was measured using a time-resolved fluoroimmunoassay (TR-FIA) for porcine relaxin that we developed. The presence of relaxin immunoreactivity was demonstrated in seminal plasma from bulls, rams and he-goats. The level of immunoreactive relaxin in seminal plasma was the highest in bulls followed by humans, rams, boars and he-goats in that order, when relaxin levels in boar and human semen having normal sperm motility were also assayed under the same conditions. When the correlation between the seminal plasma level of immunoreactive relaxin and sperm motility was examined in bull semen samples as an index for predicting fertilizing ability, it was found that the relaxin level was significantly correlated with the percentage of spermatozoa showing the most intensive motility (r = 0.64, p < 0.05). These results indicate that immunoreactive relaxin is widely found in the seminal plasma of domestic animals and that measuring the relaxin concentration of seminal plasma may be useful to identify subfertile sires or predict the fertility potential of individual sires.

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Section: Discussionmentioning

confidence: 99%

Seminal immunoreactive relaxin in domestic animals and its relationship to sperm motility as a possible index for predicting the fertilizing ability of sires

et al. 2003

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“…Therefore, the present findings indicate that immunoreactive relaxin in seminal plasma may relate to its significance not only on sperm motility, but also on sperm fertility potential. Indeed, it has been shown that incubation of mouse spermatozoa with porcine relaxin increases the rate of in vitro fertilization of mouse oocytes ( Pupula et al ., 1986 ) and that porcine relaxin enhances the penetration capacity of human spermatozoa in the zona‐free hamster egg penetration test ( Park et al ., 1988 ). In bulls, the level of a 30‐kDa heparin‐binding protein known as fertility‐associated antigen in seminal plasma has been reported to show a strong correlation with sperm fertility ( Bellin et al ., 1998 ).…”

Section: Discussionmentioning

confidence: 99%

Immunoreactive relaxin in seminal plasma of fertile boars and its correlation with sperm motility characteristics determined by computer‐assisted digital image analysis

Sasaki¹,

Kohsaka²,

Kawarasaki³

et al. 2001

Int J of Andrology

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Ejaculates from 10 mature fertile large white Yorkshire boars were used to examine the correlation between immunoreactive relaxin levels in seminal plasma and sperm motility characteristics. Seminal plasma levels of immunoreactive relaxin were measured by a time-resolved fluoroimmunoassay (TR-FIA). Motility characteristics were assessed using a CellSoft computer-assisted digital image analysis system. The mean +/- SD level of immunoreactive relaxin in seminal plasma was 2.61 +/- 0.62 ng/mL. When the correlation between seminal plasma levels of immunoreactive relaxin and parameters of sperm movement was examined, it was found that relaxin levels were significantly correlated with the percentage of motile spermatozoa (r=0.687, p < 0.05), curvilinear velocity (r=0.745, p < 0.05), straight line velocity (r=0.651, p < 0.05), mean amplitude of lateral head displacement (mean ALH) (r=0.844, p < 0.01) and the maximum amplitude of lateral head displacement (max ALH) (r=0.830, p < 0.01), but not with linearity, beat-cross frequency, or percentage of circular cells. Among these parameters, seminal plasma levels of immunoreactive relaxin showed the strongest correlation with the ALH parameter related to fertilizing ability. These results indicate that immunoreactive relaxin in boar semen may be necessary not only for normal sperm motility but also for normal fertility, suggesting that determination of the profile of immunoreactive relaxin in ejaculates may have value as a potential marker for predicting sperm fertilizing ability of boars.

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“…We observed in a previous study that relaxin has physiological roles in influencing motility and the acrosome reaction (AR) of fresh boar spermatozoa 4 . Relaxin also increases the fertilization rate of mouse 5 and porcine oocytes 6 in vitro and enhances the penetration capability of porcine as well as human spermatozoa 7 . However, relaxin is adversely affected when semen is washed and or cryopreserved, 8 which might lead to lower sperm activities 9,10 .…”

Section: Introductionmentioning

confidence: 99%

Effect of relaxin on motility, acrosome reaction and viability of cryopreserved boar spermatozoa

Miah

Hossain

Tareq

et al. 2006

Reprod Medicine & Biology

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Relaxin has an important role in stimulating motility and the acrosome reaction (AR) of fresh boar spermatozoa. The objective of the present study was to determine whether relaxin can improve the motility, AR and viability of cryopreserved boar spermatozoa. Cryopreserved boar spermatozoa were thawed, washed and incubated at 37°C for 4 h in modified Beltsville thawing solution supplemented with 0, 20 or 40 ng/mL relaxin. Sperm motility, AR, viability, and incorporation and oxidation ofC-glucose were evaluated during 0-4 h of incubation. The results show that the supplementation of relaxin (especially at 20 ng/mL) in the thawing solution improved sperm motility significantly ( < 0.05) at 1-3 h of incubation. The percentage of acrosome reacted live spermatozoa was improved significantly ( < 0.05) when the spermatozoa were treated with 20 ng/mL relaxin. Viability was not significantly ( > 0.05) improved by supplementation with relaxin. The rates of incorporation and oxidation of C-glucose were increased in correlation with AR up to 4 h of incubation. We conclude that relaxin can improve the sperm motility and AR, and enhance the glucose metabolism of cryopreserved boar spermatozoa. (Reprod Med Biol 2006; : 215-220).

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Effects of relaxin on the fertilization capacity of human spermatozoa

Cited by 5 publications

References 0 publications

Seminal immunoreactive relaxin in domestic animals and its relationship to sperm motility as a possible index for predicting the fertilizing ability of sires

Seminal immunoreactive relaxin in domestic animals and its relationship to sperm motility as a possible index for predicting the fertilizing ability of sires

Immunoreactive relaxin in seminal plasma of fertile boars and its correlation with sperm motility characteristics determined by computer‐assisted digital image analysis

Effect of relaxin on motility, acrosome reaction and viability of cryopreserved boar spermatozoa

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