Effects of recombinant IL-4δ2 on human peripheral blood mononuclears

Silkov, A. N.; Gavrilenko, V. G.; Denisova, Vera; Гришина, Л. В.; Козлов, В. А.; Сенников, С. В.

doi:10.1007/s10517-007-0020-3

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2012

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“…All donors signed informed consent for sampling biological material and received necessary explanations concerning the purposes of the study. Pe ri pheral blood mononuclear cells (PB MNC) were isolated and cultured using routine methods [2].…”

Section: Methodsmentioning

confidence: 99%

Tissue-Specific Expression of Splice Variants of Human IL-4 and IL-6 Gene mRNA

Yatsenko¹,

Silkov²,

Khrapov

et al. 2012

Bull Exp Biol Med

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The spectrum of alternatively spliced IL-4 and IL-6 gene mRNA was studied in peripheral blood mononuclears from healthy donors and in human fetal tissues. It was found that the expression of alternatively spliced IL-4 and IL-6 gene mRNA in fetal tissues is tissue specific and that hemopoiesis- and immunopoiesis-related tissues differ by the amount of IL-4 and IL-4δ2 mRNA. An mRNA variant IL-4alt3 carrying partial exon 3 deletion was for the first time identified in human mononuclear cells.

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Section: Methodsmentioning

confidence: 99%