Effects of Rare Earth Compounds on Growth and Apoptosis of Leukemic Cell Lines

Abstract

To explore a new agent for inhibiting leukemic cells, we investigated the effects of rare earth compounds (lanthanum chloride and cerium chloride) on the growth and apoptosis of HL-60 and NB4 cells. The growth of HL-60 and NB4 cells was tested by 3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide (MTT) colorimetric assay. The apoptosis was measured by light microscopy, flow cytometry, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick-end labeling (TUNEL) method. The effect of LaCl(3) on normal bone marrow hematopoietic progenitor cells was evaluated by colony-forming unit-granulocyte-macrophage (CFU-GM) assay. Under our experimental conditions, MTT assay showed that 48-h treatment with 1, 2, and 3 mM LaCl(3) or 48- and 72-h treatments with 1 mM LaCl(3) could significantly inhibit the growth of HL-60 cells. Treatment with 2 and 4 mM CeCl(3) for 72 h could significantly inhibit the growth of NB4 cells. Apoptosis could be detected on treatment with 2 mM LaCl(3) for 24 h in HL-60 cells by light microscopic morphology examination, flow cytometric analysis, and TUNEL method. Apoptosis could be also detected on treatment with 2 mM CeCl(3) for 72 h in NB4 cells. Treatment with 1 mM LaCl(3) could arrest the transitions from G0/G1 to S phase. The granulocyte-macrophage colony formation of normal bone marrow cells was not significantly inhibited at lower concentrations of LaCl(3) (0.5 to 2 mM). Our results indicate that at certain concentrations, the rare earth compounds may inhibit the growth of leukemic cells, induce them to apoptosis, and have no significant inhibitory effects on normal bone marrow hematopoietic progenitor cells (CFU-GM). The mechanism needs to be further investigated.

Evaluation of short-term effects of rare earth and other elements used in magnesium alloys on primary cells and cell lines☆

“…Additionally, evidence is rising that many REE exhibit anti-cancerogenic properties [24][25][26][27] . Therefore, magnesium alloys containing REE could be of particular interest for bone cancer treatments.…”

Corrosion protection afforded by praseodymium conversion film on Mg alloy AZNd in simulated biological fluid studied by scanning electrochemical microscopy

“…In biomedical application, use of RE compounds should also be considered from the perspective of their potential cytotoxicity. Anti-carcinogenic properties of REs has been shown in number of studies [18][19][20][21], however some studies have also reported cytotoxic and hepatotoxic effects at high dose [22][23][24][25]. It has been shown that rare-earth alloying elements including Ce, Nd, Y, and Yb have no adverse effect on growth of living cells but they can induce inflammatory effects at high concentration [26].…”

Magnesium alloys as implant materials – Principles of property design for Mg–RE alloys☆

“…Additionally, evidence is rising that many rare earth elements exhibit anticarcinogenic properties, which could lead to multifunctionailty of the designated alloys [56][57][58][59] . On the other hand it is also known that Gd based contrast agents are widely used in magnetic resonance imaging (MRI) as contrast media [60][61][62] .…”