Effects of radiation on the expression of adhesion molecules and cytokines in a static model of human dermal microvascular endothelial cells

Abstract

BACKGROUND: Radiation-induced wound healing complications represent an important clinical problem. Microvascular compromise is an important component of its pathogenesis and the microvascular endothelial cell is the key representative affected at the cellular level. MATERIAL AND METHODS: Human dermal microvascular endothelial cells (HDMEC) were cultured and irradiated with doses of 2 to 12 Gy. Cell density was determined 48 h after radiation using a semi-automated cell counting system. Levels of interleukin-6 (IL-6), basic fibroblast growth factor (FGF), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the supernatants of HDMEC were determined by polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). Non irradiated HDMEC were used as controls. RESULTS: Cell density was significantly impaired in irradiated cells compared to non irradiated controls. Radiation resulted in significant elevation of levels of IL-6, FGF, ICAM-1 and VCAM-1 in the supernatants of HDMEC in a dose dependent manner. CONCLUSION: The inflammatory response observed clinically after radiation seems to correlate with elevated expression of cytokines and adhesion molecules by microvascula endothelial cells. The model of HDMEC documents the impairment of microcirculation. These in vitro changes may enhance our understanding of the pathomechanisms leading to radiation-induced vasculitis and associated wound healing problems.

A Co-Culture Model of Fibroblasts and Adipose Tissue-Derived Stem Cells Reveals New Insights into Impaired Wound Healing After Radiotherapy

“…The other explanatory model is focused on fibroblast dysfunction because fibroblasts from irradiated tissues seem to be characterized by impaired proliferation rates [ 24 ]. These theories are supported by the experimental results of our present study on fibroblast proliferation and our previous results concerning endothelial dysfunction after external radiation [ 11 ].…”

“…Previous investigations of our group were focused on effects of external radiation on HDMECs [ 11 , 15 ]. The present study revealed a significant reduction of cell proliferation after external radiation of NHFs.…”

“…In previous experiments with human dermal microvascular endothelial cells (HDMEC), we detected elevated concentrations of pro-inflammatory mediator molecules (cytokines/chemokines) in irradiated cell culture supernatants. [ 11 ].…”

Hyperspectral Imaging as an Early Biomarker for Radiation Exposure and Microcirculatory Damage

“…The current study demonstrated that over a large exposure gradient, the initial radiation dose appears to be directly correlated to the degree of microvascular injury. This finding is consistent with results by Haubener et al who showed that varying doses of single fraction radiation exposure had an increasing effect on diminishing endothelial cell number in vitro ( 26 ).…”

Injektion von Bauchfett bei radiogenen Wundheilungsstörungen

“…Dies kann zu einer anhaltenden entzündlichen Gewebeveränderung mit dem Auftreten chronischer Wunden führen. Anhand von Zellkulturmodellen konnte unsere Arbeitsgruppe bereits die proinflammtorischen Zellreaktionen nach externer Bestrahlung dokumentieren [8,9]. Rigotti et al [ tischen Gesichtschirurgie [23,24] seit mehr als 20 Jahren dokumentiert und fanden bereits im 19.…”