Effects of radiation on chondrocytes in culture

Matsumoto, Tomoko; Iwasaki, Katsurou; Sugihara, Hajime

doi:10.1016/8756-3282(94)90898-2

Cited by 17 publications

(16 citation statements)

References 10 publications

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“…To investigate whether cell proliferation was required, we x-irradiated chondrocytes with a single 50-Gy dose, which blocks proliferation but does not kill the cells (21,22). Under our conditions, chondrocytes that had been made unable to proliferate by x-irradiation could still generate a cartilage implant in vivo (data not shown).…”

Section: Resultsmentioning

confidence: 99%

“…To obtain nonviable chondrocytes, cell suspensions were snap-frozen and thawed at 37°C for 3 minutes for 3 consecutive times as described in detail elsewhere (20). To arrest cell division while preserving cell viability, chondrocytes were x-irradiated with a single radiation dose of 50 Gy (21,22). 35 S-sulfate incorporation and hydrodynamic profile of proteoglycans.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular markers predictive of the capacity of expanded human articular chondrocytes to form stable cartilage in vivo

Dell’Accio¹,

Bari²,

Luyten³

2001

Arthritis & Rheumatism

300

217

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Molecular markers predictive of the capacity of expanded human articular chondrocytes to form stable cartilage in vivo

Dell’Accio¹,

Bari²,

Luyten³

2001

Arthritis & Rheumatism

300

217

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“…Furthermore, glycosaminoglycan synthesis is not affected by a single radiation dose at either 2 or 10 Gy, nor did glycosaminoglycan content decrease in the three-dimensional tube cultures. These results show that low-LET radiations affect chondrocytes proliferation and differentiation pathways differently, and that synthesis of extracellular matrix may be relatively more radioresistant than DNA synthesis (54). Cartilage irradiation with high-LET (RBE .…”

Section: Effect Of Irradiation On Cartilage Maturationmentioning

confidence: 97%

“…From these studies, growth plate chondrocytes sensitivity to low-LET radiation depends on their differentiation level, while articular chondrocytes are not as sensitive. Moreover, using chondrocytes from rabbit articular cartilage cultured in monolayer or in pellets with matrix (three-dimensional culture), Matsumoto and colleagues show a direct effect of single dose X irradiation at either 2 or 10 Gy on both DNA and glycosaminoglycan synthesis (54). After 2 Gy exposure, DNA synthesis is temporarily suppressed while it is definitively suppressed at 10 Gy exposure.…”

Section: Effect Of Irradiation On Cartilage Maturationmentioning

confidence: 97%

Impact of Therapeutic Irradiation on Healthy Articular Cartilage

Saintigny¹,

Cruet-Hennequart²,

Hamdi³

et al. 2015

Radiation Research

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Radiation-induced complications in bone and cartilage are of increasing concern due to potential long-term effects in cancer survivors. Healthy articular cartilage may be exposed to radiation during either chondrosarcoma treatment or in-field radiotherapy of tumors located in close proximity to articulation. Cartilage exposed to radiation undergoes bone differentiation and senescence, which can lead to painful and disabling sequelae that can impair patient quality of life. An understanding of the biological processes involved in healthy cartilage response to radiotherapy may not only optimize the delivery of therapeutic radiation but also reduce the risk of long-term sequelae in irradiated cartilage. Over the last few decades, radiobiology studies have focused primarily on signaling and repair of DNA damage pathways induced by ionizing radiation in immortalized cells under conditions dramatically different from human homeostasis. This research needs to be continued and broadened, since the range of normal tissue responses to radiation exposure is still not fully understood, despite being recognized as the major limiting factor in the rupture of tissue homeostasis after radiotherapy. Human articular cartilage is an avascular tissue with low intracellular oxygen levels and is comprised of a single cell lineage of chondrocytes embedded in a highly dense and structured extracellular matrix. These relatively unique features may impact inherent cell radiation sensitivity and suggests that canonical cell responses to ionizing radiation may not be applicable to articular cartilage. Despite the number of studies in this field, radiation-induced modifications of chondrocyte proteome remain unclear because of the dramatic variability in reported experimental conditions. In this review, we propose to introduce cartilage tissue physiology and microenvironment concepts, and then present a comprehensive synthesis of cartilage radiation biology.

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“…Irradiation induces HA chain fragmentation, and dose-dependently affects the three-dimensional polymeric structure of HA (63,64). Thus, irradiation induces a synchronous disruption and degradation of HA, but does not interfere with HA synthesis (65)(66)(67). Importantly, 4MU exerts inhibitory effects on HA synthesis by effectively depleting cellular UDP-GlcA, one of the two substrates needed for HA synthesis (23) and causes a profound disruption of HA synthesis in vitro (68).…”

Section: Discussionmentioning

confidence: 99%

Hyaluronan Expressed by the Hematopoietic Microenvironment Is Required for Bone Marrow Hematopoiesis

Goncharova

Serobyan

Iizuka

et al. 2012

Journal of Biological Chemistry

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Background: Hyaluronan (HA) contributes to the extracellular matrix in bone marrow. Results: HA expressed by the hematopoietic microenvironment supports hematopoiesis and is involved in hematopoietic stem/progenitor cell migration by regulating the production of soluble factors. Conclusion: Endogenous HA is an important regulatory element of the hematopoietic microenvironment. Significance: Understanding the biology of HA may help to develop strategies for improving the quality of the stem cell microenvironment.

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Effects of radiation on chondrocytes in culture

Cited by 17 publications

References 10 publications

Molecular markers predictive of the capacity of expanded human articular chondrocytes to form stable cartilage in vivo

Molecular markers predictive of the capacity of expanded human articular chondrocytes to form stable cartilage in vivo

Impact of Therapeutic Irradiation on Healthy Articular Cartilage

Hyaluronan Expressed by the Hematopoietic Microenvironment Is Required for Bone Marrow Hematopoiesis

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