Effects of r‐metHuG‐CSF on polymorphonuclear leucocyte kinetics and function in patients on continuous ambulatory peritoneal dialysis

Abstract

Summary. End-stage renal failure (ESRF) patients undergoing continuous ambulatory peritoneal dialysis (CAPD) are immunocompromised and exhibit abnormal circulating polymorphonuclear leucocyte (PMN) function, including reduced phagocytosis and intracellular killing. Six uraemic patients on CAPD were each given 300 mg granulocytecolony stimulating factor (G-CSF) every day for 5 d and PMN function tests were performed daily. By day 5 of the study CD11b expression was significantly decreased in response to N-formylmethionylleucylphenylalanine (fMLP) and opsonized Staphylococcus epidermidis stimulation, and expression of L-selectin (CD62L) was significantly decreased in response to opsonized Staphylococcus epidermidis stimulation. Further, superoxide anion production and FcgRI (CD64) expression were found to be significantly increased and FcgRIII (CD16) expression was lowered. Circulating white cell and PMN counts were significantly elevated in response to treatment. Administration of G-CSF did not appear to have corrected the abnormalities in phagocytosis and intracellular killing. This study suggests that G-CSF does no harm to ESRF patients and influences uraemic PMN function in a manner that is comparable to its effects on PMN in non-uraemic subjects.

Phenotype markers and function of neutrophils in children with hemolytic uremic syndrome

“…In contrast, PMN from CUC children showed increases in both cytotoxicity indexes. This is in agreement with previous reports, which have demonstrated that PMN from uremic patients were primed to increase oxygen radical production [37].…”

Μελέτη Της Φαγοκυττάρωσης Και Της Χημειοταξίας Σε Σηπτικούς Ασθενείς Και Η Επίδραση Των Αυξητικών Παραγόντων Σε Ουδετεροπενικούς Ασθενείς Με Σήψη

“…Επίσης η έκφραση του CD64 τόσο στους υγιείς, όσο και στους ασθενείς με υπερέκφραση του CD64 δεν μεταβάλλεται λόγω κυτταρικής ενεργοποίησης από χημειοτακτικούς παράγοντες, PMA ή vitro έκθεση σε βακτήρια (197,198,205,206).…”