Effects of Quizalofop-p-Ethyl Herbicide on the Somatic Chromosomes of Helianthus annuus (Sunflower)

Karaismailoğlu, Mehmet Cengiz; İnceer, Hüseyin; Hayırlıoğlu-Ayaz, Sema

doi:10.5053/ekoloji.2013.896

Cited by 9 publications

(21 citation statements)

References 30 publications

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“…Thus, assessment of the process pathways of prometryne and its effect on mitotic index, chromosomes and DNA, as applied in this investigation, provide helpful data about the effects of these commonly applied toxic substances (Turkoglu 2012). Mitotic index may be utilized as a biological marker of cell increase, which measures the percentage of cells in different mitotic stages (Ping et al 2012;Karaismailoglu et al 2013;Karaismailoglu 2014a). The effects of various concentrations of prometryne on MI in A. cepa root tip cells are presented in Table 1.…”

Section: Discussionmentioning

confidence: 99%

“…Next they were washed with fixative free distilled water, were hydrolyzed in 1 N HCl at 60°C for 10 min and stained with Schiff's reagent for 1.5 h in the dark. Five slides were chosen randomly from each application group for the cytological analysis and mitotic index (MI), micronucleus (MN) in interphase, and chromosome aberrations such as disturbed prophase, chromatid bridge, stickiness, c-mitosis and laggards were investigated in the dividing cells (Karaismailoglu et al 2013;Karaismailoglu 2014aKaraismailoglu , 2014b.…”

Section: Methodsmentioning

confidence: 99%

“…Pesticides are utilized in agricultural fields to prevent diseases caused by pests as well as during storage of agricultural products (Karaismailoglu et al 2013). Pesticides and their derivatives (or products contaminated with pesticides) accumulate in living systems and induce hazards of mutagenicity, carcinogenicity or teratogenicity (Liman et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Plants are helpful for monitoring toxic impacts on living systems of the chemical agents (Singh et al 2008) by observing chromosomal aberrations, nuclear DNA amount and micronuclei tests. The most common test plants are Allium cepa (Turkoglu 2009;Liman et al 2011; Oyeyemi and Bakare 2013; Firbas and Amon 2014), Vicia faba (Khadra et al 2012) and Helianthus annuus (Karaismailoglu et al 2013). In particular, A. cepa is one of the most frequently used plants in toxicity tests because it is a highly sensitive and reliable test organism (Grant 1994).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the potential toxic effects of prometryne herbicide onAllium ceparoot tip cells with mitotic activity, chromosome aberration, micronucleus frequency, nuclear DNA amount and comet assay

Karaismailoğlu

2015

Caryologia

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In the present study the potential toxic effect of prometryne herbicide was examined by investigating mitotic index, mitotic phase, chromosomal abnormalities, micronucleus frequency, 2C DNA content, and comet assay on the root tip cells of Allium cepa. The roots were exposed to 2, 3, 4 and 6 g l −1 concentrations within 12, 24 and 36 h. Mitotic index was markedly reduced by prometryne in each treatment group compared with the controls. The frequencies of mitotic phases have been changed. Prometryne significantly increased the anomaly cell percentage at all concentrations and application periods in comparison to their control. Mitotic abnormalities were recorded as disturbed prophase, c-mitosis, stickiness, laggards and chromatid bridges. The micronucleus was observed at interphase and its frequency was calculated in the test solutions used. Prometryne decreased the 2C DNA amount in onion root tips. Furthermore, the comet assay was applied to analyze the single stand breakages. Nearly all of the applied concentrations of prometryne enhanced DNA damage. In many cases negative correlation was found between 2C DNA amount and DNA damage; however, positive correlation was found between micronucleus formation and DNA damage.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation of the potential toxic effects of prometryne herbicide onAllium ceparoot tip cells with mitotic activity, chromosome aberration, micronucleus frequency, nuclear DNA amount and comet assay

Karaismailoğlu

2015

Caryologia

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“…Nuclear erosion, which may result from the disintegration of chromatid proteins, represents irreversible toxicity (Karaismailoglu et al, 2013). Another significant chromosomal aberration observed was hyperchromasia, which is a characteristic of programmed cell death (Soltys et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Genotoxic potential of Cynanchum sarcomedium Meve & Liede coupled with its modulatory action on oxidative-stress–mediatedgenotoxicity by hydrogen peroxide

BHAGYANATHAN¹,

THOPPIL²

2016

Turk J Biol

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The present study evaluated the genotoxic effect of aqueous extract of C. sarcomedium Meve & Liede and its protective role in chromosomal aberrations induced by 2% hydrogen peroxide (H 2 O 2 ) on Allium cepa root meristem. Our results showed that the C. sarcomedium extract induced major chromosomal aberrations, viz., nuclear lesions, chromosome breaks, chromosome laggards, vagrance, stickiness, and bridges. These are irreversible in nature and indicate that the plant is a potent clastogen. Total chromosome aberrations were more frequent with increase in exposure time and concentrations of plant extract. For antigenotoxic screening, three different modes of treatment were used: pretreatment, posttreatment, and simultaneous treatment. The significant induction of a wide spectrum of chromosomal aberrations and the reduction in mitotic index effected by the aqueous extract reinforced the genotoxic potential of the plant. The modulatory effect of plant extract was expressed in terms of inhibition percentage and had an excellent effect in reversing chromosomal aberrations induced by H 2 O 2 . Dose-dependent results suggest the genotoxic and antigenotoxic effect of C. sarcomedium on onion root tips. These findings offer a useful basis for further investigations on C. sarcomedium, a shrub with a broad array of prospective therapeutic activities.

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Assessments on the potential genotoxic effects of fipronil insecticide onAllium cepasomatic cells

Karaismailoğlu

2017

Caryologia

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The potential genotoxic influences of fipronil insecticide were analyzed by studying mitotic index and phases, chromosomal abnormalities, and micronucleus percentage on the somatic cells of Allium cepa L. The roots were treated with 1, 2.5, 5 and 10 ppm of fipronil insecticide within 6, 12 and 24 h. Mitotic index was clearly diminished by fipronil in each treatment group in comparison with control. The percentages of mitotic stages have been significantly affected. Fipronil markedly enhanced the abnormality cell percentage in almost all of the concentrations and treatment times compared to the control. Chromosomal abnormalities were recorded as disturbed prophase, sticky, chromatid bridges, c-mitosis, and laggards. The micronucleus was found at interphase and its frequency was calculated at each concentration and in the control. Generally, micronucleus formation augmented with increasing concentration of fipronil as compared to control. Consequently, the genotoxic potency of fipronil insecticide with different assessments was evaluated by using the somatic chromosomes of A. cepa, and the use of a defined non-toxic dose was suggested.

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Effects of Quizalofop-p-Ethyl Herbicide on the Somatic Chromosomes of Helianthus annuus (Sunflower)

Cited by 9 publications

References 30 publications

Investigation of the potential toxic effects of prometryne herbicide onAllium ceparoot tip cells with mitotic activity, chromosome aberration, micronucleus frequency, nuclear DNA amount and comet assay

Investigation of the potential toxic effects of prometryne herbicide onAllium ceparoot tip cells with mitotic activity, chromosome aberration, micronucleus frequency, nuclear DNA amount and comet assay

Genotoxic potential of Cynanchum sarcomedium Meve & Liede coupled with its modulatory action on oxidative-stress–mediatedgenotoxicity by hydrogen peroxide

Assessments on the potential genotoxic effects of fipronil insecticide onAllium cepasomatic cells

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