Effects of Quercetin on Mushroom Tyrosinase and B16-F10 Melanoma Cells

Kubo, Isao; Nitoda, Teruhiko; Nihei, Ken Ichi

doi:10.3390/12051045

Cited by 51 publications

(29 citation statements)

References 35 publications

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“…The presence of quercitrin and rutin, derivatives of quercetin, might be related to the capability of this extract to inhibit tyrosinase and scavenge the DPPH activities. Kubo et al [19] reported the melanin biosynthesis inhibitory activity of quercetin. Rutin was reported to have antioxidant properties [20].…”

Section: Resultsmentioning

confidence: 98%

Evaluation of medicinal plants from Central Kalimantan for antimelanogenesis

Arung

Kusuma

Christy³

et al. 2009

J Nat Med

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In the course of searching for new materials to use as whitening agents, we screened 19 methanol extracts prepared from 14 medicinal plants from Central Kalimantan province, Indonesia. The screening methods used were the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, a tyrosinase inhibition assay, and a melanin formation inhibition assay using B16 melanoma cells. The extracts of Willughbeia coriacea (bark part of aerial root), Phyllanthus urinaria (root), Eleutherine palmifolia (bulb), Eusideroxylon zwageri (seed), Dendrophthoe petandra (aerial root), Passiflora foetida (stem), and Vitex pinnata (root) showed DPPH radical-scavenging activity of more than 70% at 100 microg/ml. The extracts of W. coriacea (bark part of aerial root), P. urinaria (root), and D. petandra (aerial root) showed tyrosinase inhibitory activity of more than 40% using L-tyrosine as a substrate at 500 microg/ml. The extracts of W. coriacea (bark part of aerial root) and D. petandra (aerial root) showed tyrosinase inhibitory activity of more than 40% using L-DOPA as a substrate at 500 microg/ml. The extracts of W. coriacea (bark part of aerial root, 200 microg/ml), Glochidion philippcum (aerial root, 200 and 300 microg/ml), E. palmifolia (bulb, 50 microg/ml), E. zwageri (seed, 100 microg/ml), D. petandra (aerial root, 200 microg/ml), Lansium domesticum (bark, 25 microg/ml), P. foetida (stem, fruit, 300 microg/ml), and Solanum torvum (root, 300 microg/ml) strongly inhibited the melanin production of B16 melanoma cells without significant cytotoxicity. These findings indicate that some medicinal plants from Central Kalimantan are potential ingredients for skin-whitening cosmetics if their safety can be confirmed.

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Section: Resultsmentioning

confidence: 98%

Evaluation of medicinal plants from Central Kalimantan for antimelanogenesis

Arung

Kusuma

Christy³

et al. 2009

J Nat Med

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“…Enzymatic action catalyzed by tyrosinase utilizes quercetin as a substrate, which can form reactive o-quinone compounds (82). The activation of quercetin into quinone compounds has been observed in various cell lines, including melanoma cells (10, 11). Subsequently, after the formation of reactive species and metabolized quercetin, the compounds can bind to and deplete GSH, the main bioreductive antioxidant agent to prevent cellular damage (83).…”

Section: Bioreduction Potential and Associated Signaling Cascadesmentioning

confidence: 99%

“…Melanocytes and melanoma cells, in particular, have a unique feature in that they specifically express the oxidative enzyme tyrosinase, which can oxidize quercetin into reactive adducts (10, 11). In this review, we propose that quercetin can be used as a basis for the development of a strategy for melanoma therapeutics.…”

Section: Introductionmentioning

confidence: 99%

Quercetin as an Emerging Anti-Melanoma Agent: A Four-Focus Area Therapeutic Development Strategy

et al. 2016

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Replacing current refractory treatments for melanoma with new prevention and therapeutic approaches is crucial in order to successfully treat this aggressive cancer form. Melanoma develops from neural crest cells, which express tyrosinase – a key enzyme in the pigmentation pathway. The tyrosinase enzyme is highly active in melanoma cells and metabolizes polyphenolic compounds; tyrosinase expression thus makes feasible a target for polyphenol-based therapies. For example, quercetin (3,3′,4′,5,7-pentahydroxyflavone) is a highly ubiquitous and well-classified dietary polyphenol found in various fruits, vegetables, and other plant products including onions, broccoli, kale, oranges, blueberries, apples, and tea. Quercetin has demonstrated antiproliferative and proapoptotic activity in various cancer cell types. Quercetin is readily metabolized by tyrosinase into various compounds that promote anticancer activity; additionally, given that tyrosinase expression increases during tumorigenesis, and its activity is associated with pigmentation changes in both early- and late-stage melanocytic lesions, it suggests that quercetin can be used to target melanoma. In this review, we explore the potential of quercetin as an anti-melanoma agent utilizing and extrapolating on evidence from previous in vitro studies in various human malignant cell lines and propose a “four-focus area strategy” to develop quercetin as a targeted anti-melanoma compound for use as either a preventative or therapeutic agent. The four areas of focus include utilizing quercetin to (i) modulate cellular bioreduction potential and associated signaling cascades, (ii) affect transcription of relevant genes, (iii) regulate epigenetic processes, and (iv) develop effective combination therapies and delivery modalities/protocols. In general, quercetin could be used to exploit tyrosinase activity to prevent, and/or treat, melanoma with minimal additional side effects.

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“…It is responsible for the formation of mammalian melanin pigments as well as enzymatic browning of fruits and vegetables. Thus, tyrosinase has received great attention, since it is known as a key enzyme in melanin biosynthesis, which plays a crucial protective role against skin photocarcinogenesis (Kubo, Nitoda, & Nihei, 2007;Xue et al, 2011). However, overproduction and accumulation of melanin in the skin can result in pigmentation disorders.…”

Section: Introductionmentioning

confidence: 99%

Tyrosinase inhibitory components of immature calamondin peel

Lou

2012

Food Chemistry

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Effects of Quercetin on Mushroom Tyrosinase and B16-F10 Melanoma Cells

Cited by 51 publications

References 35 publications

Evaluation of medicinal plants from Central Kalimantan for antimelanogenesis

Evaluation of medicinal plants from Central Kalimantan for antimelanogenesis

Quercetin as an Emerging Anti-Melanoma Agent: A Four-Focus Area Therapeutic Development Strategy

Tyrosinase inhibitory components of immature calamondin peel

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