Effects of Quercetin on Expression of Genes of Carbohydrate and Lipid Metabolism Enzymes in the Liver of Rats Receiving High-Fructose Ration

Mzhel'skaya, K V; Трусов, Н В; Guseva, G N; Aksenov, I V; Кравченко, Л В; Tutelyan, V A

doi:10.1007/s10517-019-04505-0

Cited by 5 publications

(6 citation statements)

References 12 publications

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“…37,48 The butein's inhibitory effects on SCD homologs contribute to the reduced triglycerides in C. elegans, similar to the inhibitory effects on SCD by other polyphenols, such as fisetin, hesperidin, quercetin and resveratrol derivative in rats or C. elegans. 20,32,33,49 The reduced triglyceride content by butein was not correlated with changes in fatty acid desaturation in C. elegans, which may be explained by the fact that fat-6 and fat-7 share about 85% nucleotide identity and either is responsible for the desaturation of stearic acid (18:0) to oleic acid (18:1). 25,29 The inhibition of only one SCD homolog (fat-7) by butein did not have any effects on fatty acids desaturation in C. elegans.…”

Section: Discussionmentioning

confidence: 93%

“…The fatty acid Δ9 desaturases are known targets for anti‐obesity agents due to the inhibition of fatty acid desaturation, which may lead to a reduced fat accumulation 20,29–33 . In C. elegans , palmitoyl‐CoA desaturase ( fat‐5 ) is responsible for the desaturation of palmitic acid (16:0) into palmitoleic acid (16:1), while the human stearoyl‐CoA desaturase (SCD) homologs ( fat‐6 and fat‐7 ) converts stearic acid (18:0) into oleic acid (18:1Δ9) 20,29 .…”

Section: Resultsmentioning

confidence: 99%

“…The fatty acid Δ9 desaturases are known targets for antiobesity agents due to the inhibition of fatty acid desaturation, which may lead to a reduced fat accumulation. 20,[29][30][31][32][33] In C. elegans, palmitoyl-CoA desaturase (fat-5) is responsible for the desaturation of palmitic acid (16:0) into palmitoleic acid (16:1), while the human stearoyl-CoA desaturase (SCD) homologs (fat-6 and fat-7) converts stearic acid (18:0) into oleic acid (18:1Δ9). 20,29 Butein did not influence fat-5 and fat-6 expressions, however, butein at 35 and 70 μM downregulated the fat-7 expression by 31 and 37% (p = .0249 and .0060, respectively) compared to the control (Figure 2b).…”

Section: The Effects Of Butein On Lipid Metabolism-related Enzymesmentioning

confidence: 99%

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Butein inhibits lipogenesis in Caenorhabditis elegans

et al. 2020

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Butein, a flavonoid found in annatto seeds and lacquer trees, may be used for many health benefits, including the prevention of obesity. However, its antiobesity effects are not completely understood; in particular, the effects of butein on the regulation of lipid metabolism have not been explained. Thus, the goal of the current study was to determine the effects of butein on lipid metabolism in Caenorhabditis elegans, which is a multi-organ nematode used as an animal model in obesity research. Butein at 70 μM reduced triglyceride content by 27% compared to the control without altering food intake and energy expenditure. The reduced triglyceride content by butein was associated with the downregulation of sbp-1, fasn-1, and fat-7, the lipogenesis-related homologs of sterol regulatory element-binding proteins, fatty acid synthase and stearoyl-CoA desaturase, respectively. Furthermore, fat-7 and skn-1, a homolog of nuclear respiratory factors, were identified as genetic requirements for butein's effects on triglyceride content in C. elegans. The effects of butein on sbp-1 and fasn-1 were dependent on skn-1, but the downregulation of fat-7 was independent of skn-1. These results suggest that the inhibitory effects of butein on lipogenesis are via SKN-1-and FAT-7-dependent pathways in C. elegans.

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Section: Discussionmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: The Effects Of Butein On Lipid Metabolism-related Enzymesmentioning

confidence: 99%

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Butein inhibits lipogenesis in Caenorhabditis elegans

et al. 2020

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“…In a clinical study of adults with non-alcoholic fatty liver disease, serum urate levels of participants receiving a KHK inhibitor PF-06835919 at a dose of 300 mg for 6 weeks were reduced by 11.5% compared to baseline ( Kazierad et al, 2021 ). Interestingly, quercetin has reported to suppress the mRNA expression level of KHK and several genes of carbohydrate and lipid metabolism enzymes in the liver of rats receiving high-fructose diet ( Mzhel’skaya et al, 2019 ). This result suggests that quercetin might inhibit KHK activity and reduce downstream process of fructose metabolism and urate production which could be another mechanism to reduce serum urate level.…”

Section: Effects Of Quercetin In Hyperuricemia and Gouty Arthritismentioning

confidence: 99%

A review on benefits of quercetin in hyperuricemia and gouty arthritis

Nutmakul

2022

Saudi Pharmaceutical Journal

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“…Another study indicated that intestine-specific PPARα-null mice decreased obesity-associated metabolic disorders after feeding a high-fat diet, including lower bodyweight gain, insulin sensitivity improvement, and short length of small intestines [ 18 ]. Quercetin could downregulate the hepatic mRNA expression of PPARα in mice fed high-fat diets [ 19 ] and high-fructose rations [ 20 ]. Nevertheless, the gene expressions of PPARα in the duodena, ceca, and livers in chicken embryos after LPS stimulation with or without quercetin treatment remain elusive.…”

Section: Introductionmentioning

confidence: 99%

Quercetin Alleviates Inflammation and Energy Deficiency Induced by Lipopolysaccharide in Chicken Embryos

Guo

et al. 2023

Animals

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Energy deficiency causes multiple organ dysfunctions after LPS induction. Quercetin is a phenolic compound found in herbal medicines. However, the effects of quercetin in alleviating LPS-induced energy deficiency remain unclear. In the present study, an in vivo LPS-induced inflammation model was established in chicken embryos. Specific pathogen-free chicken embryos (n = 120) were allocated to control, PBS with or without ethanol, quercetin (10, 20, or 40 nmol, respectively), and LPS (125 ng/egg) with or without quercetin groups. Fifteen day old embryonated eggs were injected with the abovementioned solutions via the allantoic cavity. On embryonic day 19, the tissues of the embryos were collected for histopathological examination using frozen oil red O staining, RNA extraction, real-time quantitative polymerase chain reaction, and immunohistochemical investigations. The glycogen and lipid contents in the liver increased after LPS stimulation as compared with the PBS group, whereas quercetin decreased the accumulation as compared with the LPS group. The mRNA expressions of AMPKα1 and AMPKα2 in the duodena, ceca, and livers were upregulated after LPS induction as compared with the PBS group, while quercetin could downregulate these expressions as compared with the LPS group. The immunopositivity of AMPKα2 in the villus, crypt, lamina propria, tunica muscularis, and myenteric plexus in the duodena and in the cytoplasms of hepatocytes significantly increased after LPS induction when compared with the PBS group (p < 0.01), whereas the immunopositivity to AMPKα2 in the quercetin treatment group significantly decreased when compared with the LPS group (p < 0.01 or p < 0.05). The LPS-induced high expressions of transcription factor PPARα and glucose transporter (SGLT1) were blocked by quercetin in the duodena, ceca, and livers. Quercetin treatment improved the LPS-induced decrease in APOA4 in the duodena, ceca, and livers. The mRNA expression of PEPT1 in the duodena and ceca increased after LPS challenge, whereas quercetin could downregulate PEPT1 gene expression. These data demonstrate that quercetin improved the energy deficiency induced by LPS in chicken embryos. The LPS-induced inflammation model was established to avoid the effect of LPS exposure from the environment and intestinal flora. The results form the basis the administration of quercetin pretreatment (in ovo infection) to improve the energy state of chicken embryos and improve the inflammation response.

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Effects of Quercetin on Expression of Genes of Carbohydrate and Lipid Metabolism Enzymes in the Liver of Rats Receiving High-Fructose Ration

Cited by 5 publications

References 12 publications

Butein inhibits lipogenesis in Caenorhabditis elegans

Butein inhibits lipogenesis in Caenorhabditis elegans

A review on benefits of quercetin in hyperuricemia and gouty arthritis

Quercetin Alleviates Inflammation and Energy Deficiency Induced by Lipopolysaccharide in Chicken Embryos

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