Effects of quantum dots on different renal proximal tubule cell models and on gel-free renal tubules generated<i>in vitro</i>

Li, Yao; Zheng, Yuangang; Zhang, Kangyi; Ying, Jackie Y.; Zink, Daniele

doi:10.3109/17435390.2011.562326

Cited by 18 publications

(20 citation statements)

References 49 publications

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“…Kidney on a chip toxicity testing focuses on assessment of proximal tubule function. In one of the rare studies on NP effects, isolated proximal renal tubules were used to assess uptake and transport of quantum dots [59].…”

Section: Renal Modelsmentioning

confidence: 99%

Comparison of conventional and advancedin vitromodels in the toxicity testing of nanoparticles

Frőhlich

2018

Artificial Cells, Nanomedicine, and Biotechnology

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Humans are exposed to a wide variety of nanoparticles (NPs) present in the environment, in consumer, health and medical products, and in food. Conventional cytotoxicity testing compared to animal testing is less expensive, faster and avoids ethical problems at the expense of a lower predictive value. New cellular models and exposure conditions have been developed to overcome the limitations of conventional cell culture and obtain more predictive data. The use of three-dimensional culture, co-culture and inclusion of mechanical stimulation can provide physiologically more relevant culture conditions. These systems are particularly relevant for oral, respiratory and intravenous exposure to NPs and it may be assumed that physiologically relevant application of the NPs can improve the predictive value of in vitro testing. Various groups have used advanced culture and exposure systems, but few direct comparisons between data from conventional cultures and from advanced systems exist. In silico models may present another option to predict human health risk by NPs without using animal studies. In the absence of validation, the question whether these alternative models provide more predictive data than conventional testing remains elusive.

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Section: Renal Modelsmentioning

confidence: 99%

Comparison of conventional and advancedin vitromodels in the toxicity testing of nanoparticles

Frőhlich

2018

Artificial Cells, Nanomedicine, and Biotechnology

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“…The cell source is limited. Further, HPTCs display interdonor variability, 10 and dedifferentiate under in vitro conditions. 5,11 Because of these problems, it would be desirable to develop protocols for the differentiation of stem cells into PTCs and other renal cell types.…”

mentioning

confidence: 99%

Human embryonic stem cells differentiate into functional renal proximal tubular–like cells

Narayanan

Schumacher

Tasnim

et al. 2013

Kidney International

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139

118

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Renal cells are used in basic research, disease models, tissue engineering, drug screening, and in vitro toxicology. In order to provide a reliable source of human renal cells, we developed a protocol for the differentiation of human embryonic stem cells into renal epithelial cells. The differentiated stem cells expressed markers characteristic of renal proximal tubular cells and their precursors, whereas markers of other renal cell types were not expressed or expressed at low levels. Marker expression patterns of these differentiated stem cells and in vitro cultivated primary human renal proximal tubular cells were comparable. The differentiated stem cells showed morphological and functional characteristics of renal proximal tubular cells, and generated tubular structures in vitro and in vivo. In addition, the differentiated stem cells contributed in organ cultures for the formation of simple epithelia in the kidney cortex. Bioreactor experiments showed that these cells retained their functional characteristics under conditions as applied in bioartificial kidneys. Thus, our results show that human embryonic stem cells can differentiate into renal proximal tubular-like cells. Our approach would provide a source for human renal proximal tubular cells that are not affected by problems associated with immortalized cell lines or primary cells.

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“…Human primary renal proximal tubular cells, the porcine proximal tubular‐derived cell line LLC‐PK1 and the murine fibroblast cell line NIH 3T3 were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) and cultivated as described . HPTC were used up to passage 5.…”

Section: Methodsmentioning

confidence: 99%

A novel design of bioartificial kidneys with improved cell performance and haemocompatibility

Kandasamy

Tasnim

et al. 2013

J Cellular Molecular Medi

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Treatment with bioartificial kidneys had beneficial effects in animal experiments and improved survival of critically ill patients with acute kidney injury in a Phase II clinical trial. However, a Phase II b clinical trial failed. This and other results suggested various problems with the current design of bioartificial kidneys. We propose a novel design to improve various properties of device, including haemocompatibility and cell performance. An important feature of the novel design is confinement of the blood to the lumina of the hollow fibre membranes. This avoids exposure of the blood to the non-haemocompatible outer surfaces of hollow fibre membranes, which usually occurs in bioartificial kidneys. We use these outer surfaces as substrate for cell growth. Our results show that commercial hollow fibre membranes can be directly applied in the bioreactor when human primary renal proximal tubular cells are grown in this configuration, and no coatings are required for the formation of robust and functional renal epithelia. Furthermore, we demonstrate that the bioreactor unit produces significant amounts of interleukins. This result helps to understand the immunomodulatory effects of bioartificial kidneys, which have been observed previously. The novel bioartificial kidney design outlined here and the results obtained would be expected to improve the safety and performance of bioartificial kidneys and to contribute to a better understanding of their effects.

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Effects of quantum dots on different renal proximal tubule cell models and on gel-free renal tubules generatedin vitro

Cited by 18 publications

References 49 publications

Comparison of conventional and advancedin vitromodels in the toxicity testing of nanoparticles

Comparison of conventional and advancedin vitromodels in the toxicity testing of nanoparticles

Human embryonic stem cells differentiate into functional renal proximal tubular–like cells

A novel design of bioartificial kidneys with improved cell performance and haemocompatibility

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