Effects of pyruvate and sucrose on acetolactate synthase activity in Lindernia species and Schoenoplectus juncoides in an in vivo assay

Uchino, Akira; Watanabe, Hiroaki

doi:10.1111/j.1445-6664.2007.00254.x

Cited by 7 publications

(4 citation statements)

References 13 publications

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“…The collected plants were assessed for sensitivity to an SU herbicide, thifensulfuron‐methyl and classified as R or S using the in vivo ALS assay described by Uchino & Watanabe (). Among the 13 sampled fields, the samples from nine fields were either all susceptible or all resistant and were analysed as SU‐R or SU‐S population.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity within and between sulfonylurea‐resistant and susceptible populations of Schoenoplectus juncoides in Japan

et al. 2013

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To reveal the effects of herbicide selection on genetic diversity in the outcrossing weed species Schoenoplectus juncoides, six sulfonylurea-resistant (SU-R) and eight sulfonylurea-susceptible (SU-S) populations were analysed using 40 polymorphic inter-simple sequence repeat loci. The plants were collected from three widely separated regions: the Tohoku, Kanto and Kyushu districts of Japan. Genetic diversity values (Nei's gene diversity, h) within each SU-S population ranged from h = 0.125 to h = 0.235. The average genetic diversity within the SU-S populations was H S = 0.161, and the total genetic diversity was H T = 0.271. Although the H S of the SU-R populations (0.051) was lower than that of the SU-S populations, the H T of the SU-R populations (0.202) was comparable with that of the SU-S populations. Most of the genetic variation was found within the region for both the SU-S and SU-R populations (88% of the genetic variation respectively). Two of the SU-R populations showed relatively high genetic diversity (h = 0.117 and 0.161), which were comparable with those of the SU-S populations. In contrast, the genetic diversity within four SU-R populations was much lower (from h = 0 to 0.018) than in the SU-S populations. The results suggest that selection by sulfonylurea herbicides has decreased genetic diversity within some SU-R populations of S. juncoides. The different level of genetic diversity in the SU-R populations is most likely due to different levels of inbreeding in the populations.

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Section: Methodsmentioning

confidence: 99%

Genetic diversity within and between sulfonylurea‐resistant and susceptible populations of Schoenoplectus juncoides in Japan

et al. 2013

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“…The biotypes were identified according to the response of the whole plant to BSM at 7.5 g ai 10a −1 in pot experiments (Uchino et al . 2007) or by the in vivo assay method based on ALS activity (Uchino & Watanabe 2007). All of the biotypes were maintained in paddy soil in 200 cm 2 Wagner pots in a greenhouse at Shizuoka, Japan, from 2003 to 2005.…”

Section: Methodsmentioning

confidence: 99%

“…1996; Uchino et al . 1999; Uchino & Watanabe 2007). In addition, a simpler method was proposed, based on the regrowth of roots or shoots from weed samples treated with SU herbicides (Boutsalis 2001; Hamamura et al .…”

Section: Introductionmentioning

confidence: 99%

Difference in ultraweak photon emissions between sulfonylurea‐resistant and sulfonylurea‐susceptible biotypes of Scirpus juncoides following the application of a sulfonylurea herbicide

Inagaki

Ishida

Uchino

et al. 2008

Weed Biology and Management

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We examined the ultraweak photon emissions from a paddy weed, Scirpus juncoides, to assess the availability of photon emissions for the identification of weed biotypes resistant to sulfonylurea herbicides. The emission intensity from the plant organs increased when treated with a sulfonylurea herbicide in a concentration-dependent manner.The increment in emissions was higher in the sulfonylurea-resistant biotypes than in the sulfonylurea-susceptible biotypes.The difference between the biotypes was greater in the culms than in the roots and remained so through the vegetative growth stage to the flowering stage.This difference was independent of the seed source or mutations in the acetolactate synthase genes of the resistant biotypes.These results suggest that the determination of ultraweak photon emissions can be a useful method for identifying the sulfonylurea-resistant biotypes of S. juncoides.

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“…), a shoot regeneration method (Ohno et al . ), an in vivo ALS assay (Uchino & Watanabe ) and DNA sequencing (Uchino et al . ) have been applied.…”

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confidence: 99%

Rapid diagnosis of sulfonylurea‐resistant Schoenoplectus juncoides [Roxb.] Palla using polymerase chain reaction–restriction fragment length polymorphism and isogene‐specific direct sequencing

Sada

Ikeda

Kizawa

2012

Weed Biology and Management

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Rapid diagnostic methods to detect known mutations in acetolactate synthase (ALS) genes that confer sulfonylurea (SU) resistance to Schoenoplectus juncoides were developed in this study. By using 11 SU‐resistant accessions (nine accessions with a Pro197 substitution in ALS1 or ALS2, one accession with an Asp376Glu substitution in ALS2 and one accession with a Trp574Leu substitution in ALS2), polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis for DNA fragments that were amplified simultaneously from genomic ALS1 and ALS2 and PCR–RFLP analysis for DNA fragments that were amplified from either of the genomic ALS1 or ALS2 were carried out. In each of the two PCR–RFLP analyses, a common PCR product was digested separately with the restriction enzymes, BspLI, MboI and MunI, in order to detect Pro197 substitutions, an Asp376Glu substitution and a Trp574Leu substitution, respectively. In each of the lanes where the detection of SU‐resistant substitutions was aimed, a specific band to suggest the existence of the said substitutions was observed in theoretically assumable ways. Separately, a direct sequencing method also was established, which was able to selectively sequence ALS1 or ALS2 from common templates containing both ALS1 and ALS2 by the isogene‐selective primers that were designed to anneal either of the ALS genes. It is expected that these methods could be used for the genetic analysis of SU‐resistant S. juncoides by providing rapid and accurate diagnosis.

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Effects of pyruvate and sucrose on acetolactate synthase activity in Lindernia species and Schoenoplectus juncoides in an in vivo assay

Cited by 7 publications

References 13 publications

Genetic diversity within and between sulfonylurea‐resistant and susceptible populations of Schoenoplectus juncoides in Japan

Genetic diversity within and between sulfonylurea‐resistant and susceptible populations of Schoenoplectus juncoides in Japan

Difference in ultraweak photon emissions between sulfonylurea‐resistant and sulfonylurea‐susceptible biotypes of Scirpus juncoides following the application of a sulfonylurea herbicide

Rapid diagnosis of sulfonylurea‐resistant Schoenoplectus juncoides [Roxb.] Palla using polymerase chain reaction–restriction fragment length polymorphism and isogene‐specific direct sequencing

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