Effects of pyrite bioleaching solution of Acidithiobacillus ferrooxidans on viability, differentiation and mineralization potentials of rat osteoblasts

Zhou, Jian; Chen, Keming; Zhi, Dejuan; Xie, Qinjian; Chen, Xian; Li, Hongyu

doi:10.1007/s12272-015-0650-3

Cited by 8 publications

(8 citation statements)

References 28 publications

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“…[ 5 ] Also, many studies show that an appropriate concentration of naringin can enhance the expression of alkaline phosphatase in cells and promote osteogenic transformation. [ 6,12,23 ] The loading of naringin on brushite also turns out to be positive in promoting the ALP activity of osteoblasts.…”

Section: Resultsmentioning

confidence: 99%

“…[5] Modern scientific and technological results reveal that a proper amount of pyrite can promote the differentiation of osteoblasts and enhance osteogenic function with better mineralization performance and higher protein expression levels of osteogenesis-related genes. [4,6] Several studies have focused on developing novel bone repair scaffolds with pyrite. The porous PDLLA/pyrite scaffolds with 5 wt% pyrite were proven to stimulate the growth of ROS17/2.8 osteoblasts.…”

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confidence: 99%

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Synthesis and Characterization of Novel Pyrite/Naringin/Brushite Composite Scaffold for Bone Tissue Engineering

Zhao

Feng²,

et al. 2022

Adv Eng Mater

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Herein, pyrite‐leaching liquid and naringin‐loaded microspheres are incorporated into brushite to prepare composite scaffolds with enhanced osteogenic properties in vitro for bone tissue engineering. The results reveal that the loading of these two medicines has no significant effect on the crystalline phase of the composite, while some changes happen to the chemical groups when the dosage of the microspheres is controlled within 1.5 wt%. After soaking in phosphate buffer for 24 days, the composite containing 0%, 0.38 wt%, 0.75 wt%, and 1.50 wt% naringin‐loaded microspheres show a weight loss rate of 19.28%, 18.81%, 16.92%, and 14.14%, respectively. Simultaneously, the soaking assay reveals that the release of naringin and Fe are both kept within a safe level. A burst release of naringin is avoided since the highest concentration of naringin is detected on day 12 after immersion. The cell experiments show that all the composites have no toxicity to osteoblasts and allow the cells to grow on the surface. Furthermore, adding naringin‐loaded microspheres to the composite (0.75–1.5 wt%) promotes the mineralization ability and the alkaline phosphatase activity of the osteoblasts. Thus, the novel pyrite/naringin/brushite composite scaffold has the potential to be used in bone tissue engineering.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Synthesis and Characterization of Novel Pyrite/Naringin/Brushite Composite Scaffold for Bone Tissue Engineering

Zhao

Feng²,

et al. 2022

Adv Eng Mater

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“…Bioleaching, as an eco-friendly and energy-saving process, is widely used in metallurgical processing. It is also utilized for in vitro studies of A. ferrooxidans [ 33 , 34 ]. In our bioleaching experiment, walnut shell powder and its phenolic constituents showed high efficiency for inhibiting the oxidation of pyrite.…”

Section: Resultsmentioning

confidence: 99%

Use of Walnut Shell Powder to Inhibit Expression of Fe2+-Oxidizing Genes of Acidithiobacillus Ferrooxidans

Liu

et al. 2016

IJERPH

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Acidithiobacillus ferrooxidans is a Gram-negative bacterium that obtains energy by oxidizing Fe2+ or reduced sulfur compounds. This bacterium contributes to the formation of acid mine drainage (AMD). This study determined whether walnut shell powder inhibits the growth of A. ferrooxidans. First, the effects of walnut shell powder on Fe2+ oxidization and H+ production were evaluated. Second, the chemical constituents of walnut shell were isolated to determine the active ingredient(s). Third, the expression of Fe2+-oxidizing genes and rus operon genes was investigated using real-time polymerase chain reaction. Finally, growth curves were plotted, and a bioleaching experiment was performed to confirm the active ingredient(s) in walnut shells. The results indicated that both walnut shell powder and the phenolic fraction exert high inhibitory effects on Fe2+ oxidation and H+ production by A. ferrooxidans cultured in standard 9K medium. The phenolic components exert their inhibitory effects by down-regulating the expression of Fe2+-oxidizing genes and rus operon genes, which significantly decreased the growth of A. ferrooxidans. This study revealed walnut shell powder to be a promising substance for controlling AMD.

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“…Cells were plated at 4.2 × 10 5 cells/well in 6-well plate (Corning, NY, USA). After seeding, cells were exposed to Fe 3+ or Fe 2+ iron donors (FAC and FAS) at 0, 100, 200 and 300 μM for 24, 48, and 72 h. Several studies showed that the expression alteration of osteoblast differentiation markers in different osteoblast cell lines including UMR-106 cells could be observed from 12 to 96 h of treatments, and the alteration of mRNA and protein expression was well correlated [38][39][40][41][42]. After incubation, the cells were collected by washing twice with phosphate buffered saline (PBS) solution and dissolved in TRIzol reagent (Invitrogen, Carlsbad, CA, USA) to extract total RNA.…”

Section: Quantitative Real-time Pcr (Qrt-pcr)mentioning

confidence: 99%

Differential effects of Fe2+ and Fe3+ on osteoblasts and the effects of 1,25(OH)2D3, deferiprone and extracellular calcium on osteoblast viability under iron-overloaded conditions

et al. 2020

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One of the potential contributing factors for iron overload-induced osteoporosis is the iron toxicity on bone forming cells, osteoblasts. In this study, the comparative effects of Fe 3+ and Fe 2 + on osteoblast differentiation and mineralization were studied in UMR-106 osteoblast cells by using ferric ammonium citrate and ferrous ammonium sulfate as Fe 3+ and Fe 2+ donors, respectively. Effects of 1,25 dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ] and iron chelator deferiprone on iron uptake ability of osteoblasts were examined, and the potential protective ability of 1,25(OH) 2 D 3 , deferiprone and extracellular calcium treatment in osteoblast cell survival under iron overload was also elucidated. The differential effects of Fe 3+ and Fe 2+ on reactive oxygen species (ROS) production in osteoblasts were also compared. Our results showed that both iron species suppressed alkaline phosphatase gene expression and mineralization with the stronger effects from Fe 3+ than Fe 2+ . 1,25(OH) 2 D 3 significantly increased the intracellular iron but minimally affected osteoblast cell survival under iron overload. Deferiprone markedly decreased intracellular iron in osteoblasts, but it could not recover iron-induced osteoblast cell death. Interestingly, extracellular calcium was able to rescue osteoblasts from iron-induced osteoblast cell death. Additionally, both iron species could induce ROS production and G0/G1 cell cycle arrest in osteoblasts with the stronger effects from Fe 3+ . In conclusions, Fe 3+ and Fe 2+ differentially compromised the osteoblast functions and viability, which can be alleviated by an increase in extracellular ionized calcium, but not 1,25(OH) 2 D 3 or iron chelator deferiprone. This study has provided the invaluable information for therapeutic design targeting specific iron specie(s) in iron overload-induced osteoporosis. Moreover, an increase in extracellular calcium could be beneficial for this group of patients.

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Effects of pyrite bioleaching solution of Acidithiobacillus ferrooxidans on viability, differentiation and mineralization potentials of rat osteoblasts

Cited by 8 publications

References 28 publications

Synthesis and Characterization of Novel Pyrite/Naringin/Brushite Composite Scaffold for Bone Tissue Engineering

Synthesis and Characterization of Novel Pyrite/Naringin/Brushite Composite Scaffold for Bone Tissue Engineering

Use of Walnut Shell Powder to Inhibit Expression of Fe2+-Oxidizing Genes of Acidithiobacillus Ferrooxidans

Differential effects of Fe2+ and Fe3+ on osteoblasts and the effects of 1,25(OH)2D3, deferiprone and extracellular calcium on osteoblast viability under iron-overloaded conditions

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