Effects of pulsed electromagnetic fields on the mRNA expression of CAII and RANK in ovariectomized rats

Chen, Jian; Huang, Liqun; Xia, Qing; He, Chengqi

doi:10.1007/s00296-010-1740-7

Cited by 9 publications

(7 citation statements)

References 43 publications

(44 reference statements)

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“…Standard ultrasonic coupling gel was applied between the transducer head and the limb to minimize reflection and attenuation of the US energy. Concurrently, rabbits in the ERT group were orally treated with conjugated estrogens tablets (Pfizer, New York, USA) at 0.625 mg/kg/day for 10 days (28,29). The OVX and control groups remained untreated.…”

Section: Animals Were Housed In Individual Cages With a 12:12-h Lightmentioning

confidence: 99%

Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase-13 expression in ovariectomized rabbits

Xia

Guo

et al. 2015

Experimental and Therapeutic Medicine

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Abstract. The aim of the present study was to observe the effect of ultrasound (US) on estradiol level, bone mineral density (BMD), bone biomechanics and matrix metalloproteinase-13 (MMP-13) expression in ovariectomized (OVX) rabbits. A total of 28 virgin New Zealand white rabbits were randomly assigned into the following groups: Control (control group), ovariectomy (OVX group), ovariectomy with ultrasound therapy (US group) and ovariectomy with estrogen replacement therapy group (ERT group). At 8 weeks after ovariectomy, the US group received ultrasound treatment while the ERT group were orally treated with conjugated estrogens, and the control and OVX groups remained untreated. The estradiol level, BMD and bone biomechanics, cartilage histology and the MMP-13 expression were analyzed after the intervention. The results indicate that the US treatment increased estradiol level, BMD and bone biomechanical function. Furthermore, the US treatment appeared to improve the recovery of cartilage morphology and decreased the expression of MMP-13 in OVX models. Furthermore, the results suggest that 10 days of US therapy was sufficient to prevent the reduction of estradiol, BMD and bone biomechanical function, to protect osteoarthritis cartilage structure, and to reduce MMP-13 transcription and expression in OVX rabbits. Therefore, US treatment may be a potential treatment for postmenopausal osteoarthritis and osteoporosis.

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Section: Animals Were Housed In Individual Cages With a 12:12-h Lightmentioning

confidence: 99%

Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase-13 expression in ovariectomized rabbits

Xia

Guo

et al. 2015

Experimental and Therapeutic Medicine

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“…As shown by clinical evidence, therapeutic effects can be achieved for union-delayed and nonunion bone fractures using PEMF treatment at any fracture location. As we previously reported [25], PEMF reduces the gene expression of RANK, CA II and NAFT2 in vitro. A study found PEMF affect only the osteoclast phenotype after short exposure times [42].…”

Section: Figurementioning

confidence: 66%

“…PEMF stimulation for 30 days restored trabecular bone mass of PEMF+OVX rats to levels equivalent to agematched intact rats. We have previously reported that PEMFs applied at 3.8 mT, 8 Hz, for 40 min per day could regulate osteoclast gene expression of CA II and RANK in vivo and in vitro [24,25].…”

Section: Introductionmentioning

confidence: 99%

Study on Pulsed Electromagnetic Fields Regulate the mRNA Expression of V-ATPase, CAII, NFAT2 and RANK in an Ovariectomized Rat Model of Osteoporosis Mechanism Analysis

Huang¹,

Chen²,

He³

et al. 2017

J Bioengineer & Biomedical Sci

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We investigated the mRNA expression of V-ATPase CA II, NFAT2, and RANK as well as bone mineral density (BMD) in bilaterally ovariectomized (OVX) rats treated with pulsed electromagnetic fields (PEMF), to explore the mechanism by which PEMF alter bone at the molecular level in vivo. 24 Sprague-Dawley female rats were randomly divided into three different groups: SHAM, OVX, ovariectomy with PEMF stimulation (PEMF+OVX). The PEMF frequency was 3.8 mT, 8 Hz, for 40 min per day. Eight rats in each group were treated for 28 days. At the end of the intervention, the body weight and BMD were measured. The mRNA expression of V-ATPase, CA II, NFAT2 and RANK were analyzed by real-time fluorescent-nested quantitative polymerase chain reaction (RT-PCR). The V-ATPase, CAII, NFAT2 and RANK genes show strong expression in the OVX group as compared with the SHAM group (P<0.001, respectively). The mRNAs expression of V-ATPase, CAII, NFAT2 and RANK in PEMF treatment group were significantly lower than that in OVX group, respectively (p=0.001, p=0.000, p=0.013, P=0.04, respectively). The V-ATPase, CAII and RANK mRNA expression in the PEMF+OVX group were higher than SHAM group (p=0.04, p=0.001, p=0.000, respectively). However, the NFAT2 mRNA expression did not show a statistically significant difference between the SHAM and the PEMF+OVX group (P=0.08). This study indicated that exposure to PEMF restrained bone resorption in order to elevate BMD, and this outcome occurred in part through RANK-signaling by the CN/NFAT pathway to regulate bone metabolism. Alternatively, PEMF may promote the BMD through regulating the expression of NFAT2 genes. It may have certain significance in the treatment target of osteoporosis.

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“…Pulsed electromagnetic fields (PEMF) have been proven to present satisfying therapeutic effects on OP experimentally and clinically 9, 12, 37, 38 . However, the potential mechanism responsible for the effects of PEMF on preservation of bone mass are not well understood.…”

Section: Discussionmentioning

confidence: 99%

Effects of four kinds of electromagnetic fields (EMF) with different frequency spectrum bands on ovariectomized osteoporosis in mice

Tao

Liang

et al. 2017

Sci Rep

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Electromagnetic fields (EMF) was considered as a non-invasive modality for treatment of osteoporosis while the effects were diverse with EMF parameters in time domain. In present study, we extended analysis of EMF characteristics from time domain to frequency domain, aiming to investigate effects of four kinds of EMF (LP (1–100 Hz), BP (100–3,000 Hz), HP (3,000–50,000 Hz) and AP (1–50,000 Hz)) on ovariectomized (OVX) osteoporosis (OP) in mice. Forty-eight 3-month-old female BALB/c mice were equally assigned to Sham, OVX, OVX + LP, OVX + BP, OVX + HP and OVX + AP groups (n = 8). After 8-week exposure (3 h/day), LP and BP significantly increased serum bone formation markers and osteogenesis-related gene expressions compared with OVX. Bedsides, LP and BP also slightly increased bone resorption activity compared with OVX, evidenced by increased RANKL/OPG ratio. HP sharply decreased serum bone formation and resporption markers and osteogenesis and osteoclastogenesis related gene expressions compared with OVX. AP had accumulative effects of LP, BP and HP, which significantly increased bone formation and decreased bone resporption activity compared with OVX. As a result, LP, BP and HP exposure did not later deterioration of bone mass, microarchitecture and mechanical strength in OVX mice with OP. However, AP stimulation attenuated OVX-induced bone loss.

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Effects of pulsed electromagnetic fields on the mRNA expression of CAII and RANK in ovariectomized rats

Cited by 9 publications

References 43 publications

Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase-13 expression in ovariectomized rabbits

Effects of ultrasound on estradiol level, bone mineral density, bone biomechanics and matrix metalloproteinase-13 expression in ovariectomized rabbits

Study on Pulsed Electromagnetic Fields Regulate the mRNA Expression of V-ATPase, CAII, NFAT2 and RANK in an Ovariectomized Rat Model of Osteoporosis Mechanism Analysis

Effects of four kinds of electromagnetic fields (EMF) with different frequency spectrum bands on ovariectomized osteoporosis in mice

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