Effects of Puerarin Combined with PLGA/TCP/Puerarin on Osteocalcin and Sialoprotein of Mandibular Defects

Guo, Ying; Zhang, Qianqian; Mi, Nu; Li, Chunmei; Lv, Xuechao; Wang, Hong

doi:10.1155/2022/5177419

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Like other osteogenic factors, it can be determined using RT-PCR and immunocytochemical staining or ELISA. 84 − 86 …”

Section: Evaluation Of Materials For the Treatment Of Osteochondral D...mentioning

confidence: 99%

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Materials Suitable for Osteochondral Regeneration

Novotná,

Franková

2024

ACS Omega

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Osteochondral defects affect articular cartilage, calcified cartilage, and subchondral bone. The main problem that they cause is a different behavior of cell tissue in the osteochondral and bone part. Articular cartilage is composed mainly of collagen II, glycosaminoglycan (GAG), and water, and has a low healing ability due to a lack of vascularization. However, bone tissue is composed of collagen I, proteoglycans, and inorganic composites such as hydroxyapatite. Due to the discrepancy between the characters of these two parts, it is difficult to find materials that will meet all the structural and other requirements for effective regeneration. When designing a scaffold for an osteochondral defect, a variety of materials are available, e.g., polymers (synthetic and natural), inorganic particles, and extracellular matrix (ECM) components. All of them require the accurate characterization of the prepared materials and a number of in vitro and in vivo tests before they are applied to patients. Taken in concert, the final material needs to mimic the structural, morphological, chemical, and cellular demands of the native tissue. In this review, we present an overview of the structure and composition of the osteochondral part, especially synthetic materials with additives appropriate for healing osteochondral defects. Finally, we summarize in vitro and in vivo methods suitable for evaluating materials for restoring osteochondral defects.

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“…Like other osteogenic factors, it can be determined using RT-PCR and immunocytochemical staining or ELISA. 84 − 86 …”

Section: Evaluation Of Materials For the Treatment Of Osteochondral D...mentioning

confidence: 99%

“…Its determination is important for assessing the ability of biomaterials to support osteogenesis. Like other osteogenic factors, it can be determined using RT-PCR and immunocytochemical staining or ELISA. − …”

Section: Evaluation Of Materials For the Treatment Of Osteochondral D...mentioning

confidence: 99%

Materials Suitable for Osteochondral Regeneration

Novotná,

Franková

2024

ACS Omega

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Puerarin rescued Dex-induced Osteoblast Apoptosis via AIF-mediated Caspase-independent pathway

Yu,

Qiao,

Chen

et al. 2024

Preprint

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GIOP is the most common secondary osteoporosis, and the increased apoptosis of osteoblasts is thought to be the main cause of GIOP. Puerarin is the major isoflavone extracted from Pueraria lobata. Our previous study indicated that Puerarin protects osteoblast apoptosis partly through the caspase-dependent pathway. So, we concluded that there existed other anti-apoptotic mechanisms in Puerarin. Therefore, in the current study, we focused on the role of the caspase-independent pathway, specifically the apoptosis-inducing factor (AIF) pathway. MTS assay, flow cytometry, TUNEL staining, Western blotting, and immunofluorescence staining were used in this study. Caspase inhibitor Z-VAD-FMK and RNA silencing (siRNA) AIF were used to assist Puerarin in detecting the anti-apoptosis mechanisms. The results showed that Puerarin significantly alleviated Dex-induced osteoblast cell proliferation inhibition and cell cycle arrest at G0/G1 phase. Puerarin can restore the protein expression of cell cycle-related proteins (cyclin D1) and cyclin-dependent kinase (CDK4). Flow cytometry and TUNEL staining revealed that the pan-caspase inhibitor Z-VAD-FMK and siRNA AIF partially inhibited Dex-induced osteoblast apoptosis, while Puerarin significantly reduced Dex-induced osteoblast cell apoptosis. Immunofluorescence and Western blotting results showed that nucleus translocation of AIF from mitochondria in Dex-treated cells also inhibited by Puerarin. Our results suggest that Puerarin can restore Dex-induced cell cycle inhibition and apoptosis of osteoblasts through the AIF-regulated caspase-independent pathway.

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Effects of Puerarin Combined with PLGA/TCP/Puerarin on Osteocalcin and Sialoprotein of Mandibular Defects

Cited by 2 publications

References 25 publications

Materials Suitable for Osteochondral Regeneration

Materials Suitable for Osteochondral Regeneration

Puerarin rescued Dex-induced Osteoblast Apoptosis via AIF-mediated Caspase-independent pathway

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