Effects of protein kinase and phosphatase inhibitors and anti-L antisera on K+ transport in LK sheep red cells

Campbell, Eldridge; Ellory, J. Clive; Gibson, John S.

doi:10.1016/s0302-4598(00)00097-0

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…GIA/RGA-like gibberellin response modulator는 신장촉 진작용, 종자발아촉진작용, 개화촉진작용, 착과의 증가작용, 열매의 생장촉진작용 등의 기능을 하는 지베렐린 작용의 조 절인자 역할을 하는 효소이다 (Petty et al, 2003). 칼륨은 식 물이 생장 및 발달을 위한 중요한 영양소 중에 하나이다 (Ashley et al, 2006;Campbell et al, 2000). 일라이트 처리 구에서 줄기조직의 K + 이온 흡수량이 대조구보다 높게 나타난 것은 potassium transport protein(spot S7)의 발현이 높았기 때문인 것으로 판단된다(Fig.…”

Section: 상토 및 식물체 분석unclassified

Effects of the Applications of Clay Minerals on the Early Growth of Red Pepper in Growing Medium

이동기¹,

이석언²,

김덕현³

et al. 2012

Korean Journal of Horticultural Science and Technology

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As the advanced seeding technology through use of plug tray for good cultivation of seeds was propagated along with the expansion and development of horticultural industry, the use of bed soils as growing medium has recently been increased. In this study, the effects of the four clay minerals such as illite, phyllite, zeolite, and bentonite on the early growth of red pepper in the bed soil were investigated. Furthermore, proteome analysis for the leaf and stem samples of red pepper treated with only illite was performed. Of the seedling cultured, the healthy and regular size seeds were selected and cultivated in the pots, after they were treated with four clay minerals. The experiment was performed during the whole six weeks in the glasshouse of the Chungbuk National University. The growth lengths, fresh and dry weights of red pepper were significantly higher in the treatments of illite, phyllite, zeolite, and bentonite than in the control. In addition, the uptake of K + , Ca 2+ , and Mg 2+ were higher in the treatment of illite, phyllite, zeolite, and bentonite than in the control. The 2-DE patterns for the red pepper by the applications of illite, phyllite, zeolite, and bentonite were similar to each other. Therefore, compared to the samples of control, the proteome analysis for the samples of red pepper treated by only illite were performed. Proteome analysis for red pepper showed that plastid fructose-1, 6-bisphosphate aldolase class 1, aldolase, and glyceraldehydes 3-phosphate dehydrogenase, all of which were involved in the energy metabolism, were highly expressed in leaf tissue by illite treatment. In stem tissue, NAD-dependent formate dehydrogenase involved in energy metabolism, potassium transport protein, and GIA/RGA-like gibberellins response modulator were highly expressed. Based on the results obtained from the proteome analysis, it appears that the proteins specifically and differentially expressed on the illite treatment may be involved in the enhanced growth of red pepper. The identification of some proteins involved in the response of vegetable crops to the treatment of clay mineral can provide new insights that can lead to a better elucidation and understanding of mechanism on their molecular basis.

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Section: 상토 및 식물체 분석unclassified

Effects of the Applications of Clay Minerals on the Early Growth of Red Pepper in Growing Medium

이동기¹,

이석언²,

김덕현³

et al. 2012

Korean Journal of Horticultural Science and Technology

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“…Physiological modulators include cell volume ( Ellory and Dunham, 1980 ; Dunham and Ellory, 1981 ), intracellular pH ( Ellory et al, 1991 ), intracellular [Cl − ] ( Voets et al, 1999 ), extracellular [K + ] and [Cl − ] ( Lauf et al, 1992 ), urea ( Dunham, 1995 ; Kaji and Gasson, 1995 ; Speake and Gibson, 1997 ), temperature ( Khan and Ellory, 2000 ) and O 2 tension ( Borgese et al, 1991 ; Nielsen et al, 1992 ; Campbell and Gibson, 1998 ; Gibson et al, 1998 ; Gibson et al, 2000 ). Using protein kinase and phosphatase inhibitors of differing degrees of specificity (such as okadaic acid, calyculin, staurosporine, genistein, imantanib, etc), pharmacological studies have shown an important role for protein phosphorylation ( Jennings and Al-Rohil, 1990 ; Jennings and Schulz, 1991 ; Starke and Jennings, 1993 ; Cossins et al, 1994 ; Flatman et al, 1996 ; Bize et al, 1999 ; Bize et al, 2000 ; Campbell et al, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

“…Over the years, the behaviour of KCC in LK sheep red cells has provided an important paradigm for that in human red cells and in other species (eg Ellory and Dunham, 1980 ; Lauf and Theg, 1980 ; Lauf et al, 1992 ; Dunham, 1995 ; Campbell and Gibson, 1998 ; Campbell et al, 2000 ), and remains a valuable model system as well as being important in sheep erythrogenesis ( Lauf and Bauer, 1987 ). It is worth noting that the genes for KCC are well conserved between human and sheep (eg for KCC1/SLC12A4 - https://www.ncbi.nim.ni.gov/gene6560 &/gene443178) and that sheep red cells have a similar low oxygen affinity to sickle cells ( Samaja and Gattioni, 1978 ; Correra et al, 1984 ), which may be relevant when considering an oxygen-sensitive membrane transport system.…”

Section: Introductionmentioning

confidence: 99%

Does Plasma Inhibit the Activity of KCl Cotransport in Red Cells From LK Sheep?

Hannemann

Gibson

2022

Front. Physiol.

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Red cells from LK sheep represent an important paradigm for control of KCl cotransport activity, as well as being important to sheep erythroid function. A previous report (Godart et al., 1997) suggested that autologous plasma markedly inhibits red cell KCC activity and identified the presence of the bicarbonate/CO2 buffer system as the probable cause. Findings were restricted, however, to red cells from patients with sickle cell disease (SCD) swollen anisotonically and carried out at a very high O2 tension (c.700 mmHg). It was therefore important to investigate the generality of the effect described and whether it was also relevant to the two main stimuli for KCC activity encountered most often by circulating red cells in vivo - low pH in active muscle beds during exercise and high urea concentrations in the renal medulla during antidiuresis. Results confirm that inhibition was significant in response to anisotonic swelling with KCC activity in MOPS-buffered saline (MBS) vs. bicarbonate-buffered saline (BBS) and in MBS vs. plasma both reduced (by about 25 and 50%, respectively). By contrast, however, inhibition was absent at low pH and in high concentrations of urea. These findings suggest therefore that red cell KCC activity represents an important membrane permeability in vivo in red cells suspended in plasma. They are relevant, in particular, to sheep red cells, and may also be important by extension to those of other species and to the abnormal red cells found in human patients with SCD.

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Regulation of K–Cl cotransport in erythrocytes of frog Rana temporaria by commonly used protein kinase and protein phosphatase inhibitors

Гусев

Agalakova

2009

J Comp Physiol B

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Recently (Agalakova and Gusev in J Comp Physiol 179:443-450, 2009), we demonstrated that the activity of K-Cl cotransport (KCC) in frog red blood cells is inhibited under stimulation of protein kinase C (PKC) with phorbol ester PMA (12-myristate-13-acetate). Present work was performed to uncover possible implication of protein kinases and protein phosphatases (PPs) in the regulation of baseline and volume-dependent KCC activity in these cells. K+ influx was estimated as 86Rb uptake by the cells in isotonic or hypotonic media in the presence of ouabain, K+ efflux was determined as the difference between K+ loss by the cells incubated in parallel in isotonic or hypotonic K(+)-free Cl(-)- and NO(3)(-)-media. Swelling of the cells in hypotonic medium was accompanied by approximately 50% activation of Cl-dependent K+ influx and efflux. Protein tyrosine kinase (PTK) inhibitor genistein (0.1 mM) stably and considerably (up to 89%) suppressed both baseline and volume-dependent KCC activity in each direction. Other PTK blockers (tyrphostin 23 and quercetin) had no influence on KCC activity in frog erythrocytes. PKC inhibitor chelerythrine (20 microM) and both PP inhibitors, fluoride (5 mM) and okadaic acid (1 microM), reduced KCC activity by 25-70%. Neither basal nor swelling-activated KCC in frog erythrocytes was affected by PKC inhibitor staurosporine (1 microM). Based on the previous and present results, we can suggest that the main role in the maintenance of basal and volume-dependent KCC activity in frog erythrocytes belongs to PTKs and PPs, whereas PKC is a negative regulator of this ion system.

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Effects of protein kinase and phosphatase inhibitors and anti-L antisera on K+ transport in LK sheep red cells

Cited by 3 publications

References 36 publications

Effects of the Applications of Clay Minerals on the Early Growth of Red Pepper in Growing Medium

Effects of the Applications of Clay Minerals on the Early Growth of Red Pepper in Growing Medium

Does Plasma Inhibit the Activity of KCl Cotransport in Red Cells From LK Sheep?

Regulation of K–Cl cotransport in erythrocytes of frog Rana temporaria by commonly used protein kinase and protein phosphatase inhibitors

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