Effects of Prostaglandins D, and E₂on Ciliary Beat Frequency of Human Upper Respiratory Ciliain Vitro

Abstract

Diminished mucociliary transport can occur in a type-I (Ig-E mediated) allergic reaction. We determined the effects of the allergy mediators prostaglandin D2 (PGD2) and prostaglandin E2 (PGE2) on the ciliary beat frequency (CBF) of human upper respiratory cilia in vitro. Human adenoid tissue was used as the source for ciliated epithelium. CBF was measured by a computerized photo-electric method. PGD2 (10(-8)-10(-5) M, n = 7) showed no statistically significant effect on CBF. PGE2 (10(-9)-10(-6) M, n = 10) caused a significant dose-dependent stimulation, with a maximum of 37% (ANOVA, p < 0.001). Thus prostaglandins D2 and E2 do not exert a direct negative influence on ciliary activity, which could account for a decrease in mucociliary transport. The stimulating effect of PGE2 may be relevant in promoting mucociliary clearance in vivo.

Effects of Thymoquinone and Montelukast on Sinonasal Ciliary Beat Frequency

“…32 Furthermore, prostaglandin E 2 has been shown to acutely decrease CBF. 33 In our study, CBF showed a statistically significant dose-dependent increase in the experimental group compared with the control group that persisted up to 24 hours.…”

Development of aXenopus-based assay for high-throughput evaluation of mucociliary flow

“…Following a systematic literature review we identified cAMP (Di Benedetto et al, 1991; Haxel et al, 2001; Runer and Lindberg, 1999; Schmid et al, 2006), IL-5 (Grosse-Onnebrink et al, 2016), IFN-γ (Grosse-Onnebrink et al, 2016), Prostaglandin-E2 (Bonin et al, 1992; Haxel et al, 2001; Schuil et al, 1995) and Sildenafil (Fowler et al, 2020), as compounds previously shown to enhance human ciliary motility either via changes in calcium levels or through various signal transduction pathways. Xenopus tadpoles (stage 34) were pre-treated with the candidate compounds, at concentrations indicated in Figure 5 , and examined for their specific effects in mucociliary flow.…”

Effects of normobaric oxygen on ciliary beat frequency of human respiratory epithelium

“…Other groups found a significant increase in CBF after exposure to different mediators related to inflammation. 16 Bonin, Phillips and McCaffrey 17 and Khan, Dolata and Lindberg 18 found that inflammatory substances (PGE 2 , prostacyclins) can upregulate CBF in respiratory cells. Substance P seems to have similar effects.…”