Effects of prostaglandin E2 on cells cultured from the rat organum vasculosum laminae terminalis and median preoptic nucleus

Simm, Björn; Ott, Daniela; Pollatzek, Eric; Murgott, Jolanta; Gerstberger, Rüdiger; Rummel, Christoph; Roth, Joachim

doi:10.1016/j.neuroscience.2015.11.034

Cited by 28 publications

(34 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We considered L-glutamate contribution to the PGE 2 /EP3 receptor-mediated pressor response because L-glutamate release within the RVLM causes pressor response (Ito et al, 2003;Rezq and Abdel-Rahman, 2016), and because PGE 2 : 1) produces Ca 12 -dependent L-glutamate release in cultured astrocytes and neurons (Bezzi et al, 1998;Wang et al, 2015); 2) enhances glutamatergic neurotransmission in primary microcultures of the rat hypothalamic structures (Simm et al, 2016); 3) augments excitotoxicity produced by N-methyl-Daspartate in mice (Iadecola et al, 2001); and 4) augments glutamate-induced excitotoxicity in hippocampal slices via EP3 receptor activation (Ikeda-Matsuo, 2013). Given the unfeasibility to measure L-glutamate level in RVLM tissue, and building on the aforementioned in vitro studies, we showed that PGE 2 caused a concentration-dependent increase in glutamate release in PC12 cells (Fig.…”

Section: Discussionmentioning

confidence: 99%

Rostral Ventrolateral Medulla EP3 Receptor Mediates the Sympathoexcitatory and Pressor Effects of Prostaglandin E₂ in Conscious Rats

Rezq

Abdel‐Rahman

2016

J Pharmacol Exp Ther

View full text Add to dashboard Cite

Whereas few studies have dealt with the central sympathoexcitatory action of the inflammatory prostanoid prostaglandin E 2 (PGE 2 ), there is no information on the expression and cardiovascular function of different PGE 2 (EP) receptors in one of the major cardiovascular-regulating nuclei, the rostral ventrolateral medulla (RVLM). The current study aimed at filling this knowledge gap as well as elucidating the implicated molecular mechanisms. To achieve these goals, we showed the expression of EP2, EP3, and EP4 receptors in the RVLM and investigated their cardiovascular roles in conscious rats, ex vivo as well as in cultured PC12 cells. Intra-RVLM PGE 2 significantly increased blood pressure and sympathetic dominance (spectral analysis). Studies with selective EP receptor subtype agonists and antagonists showed that these PGE 2 -evoked responses were only replicated by intra-RVLM activation of the EP3 receptor with its agonist sulprostone. The RVLM of PGE 2 -treated rats exhibited increases in c-Fos expression and extracellular signal-regulated kinase 1/2 and neuronal nitric oxide synthase phosphorylation along with oxidative stress, and PGE 2 increased L-glutamate release in PC12 cells (surrogates of RVLM neurons). Abrogation of the PGE 2 -evoked pressor and biochemical responses only occurred following EP3 receptor blockade (N-[(5-Bromo-2-methoxyphenyl)sulfonyl]-3-[2-(2-naphthalenylmethyl) phenyl]-2-propenamide, L-798106). These findings suggest the dependence of RVLM PGE 2 -mediated sympathoexcitation/ pressor response on local EP3 receptor signaling in conscious rats, and highlight central EP3 receptor blockade as a potential therapeutic modality for hypertension management.

show abstract

Section: Discussionmentioning

confidence: 99%

Rostral Ventrolateral Medulla EP3 Receptor Mediates the Sympathoexcitatory and Pressor Effects of Prostaglandin E₂ in Conscious Rats

Rezq

Abdel‐Rahman

2016

J Pharmacol Exp Ther

View full text Add to dashboard Cite

show abstract

“…Cytokines were measured in the supernatant of POA primary cell cultures. TNF-α and IL-6 levels were determined by bioassay, based on the cytotoxic effect of TNF-α on mouse fibrosarcoma cell line WEHI 164 subclone 13, and the dose-dependent growth stimulation of IL-6 on the B9 hybridoma cell line, as previously reported (Ott et al, 2010;Soares et al, 2013;Simm et al, 2016). The detection limits of the assays, after considering the dilution of samples, proved to be 6.0 pg/ml for TNF-α and 3.0 IU/ml for IL-6 (Ott et al, 2010).…”

Section: Measurements Of Tnf-α and Il-6mentioning

confidence: 99%

Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition

Gomes

Sousa

Ott

et al. 2019

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Cytoglobin (Cygb) is a hexacoordinate protein, associated with the transport of oxygen, nitric oxide scavenging, tumor suppression and protection against oxidative stress and inflammation. This protein is expressed in brain areas including the preoptic area (POA) of the anterior hypothalamus, the region responsible for the regulation of body temperature. In this study, we show that Cygb is upregulated in the rat hypothalamus 2.5 h and 5 h after intravenous administration of lipopolysaccharide (LPS). We investigated the effect of treatment with Cygb in POA primary cultures stimulated with LPS for 4 h. The levels of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were measured and the results showed that Cygb reduced the concentrations of both cytokines. We further observed a decrease in immunoreactivity of the inflammatory transcription factor nuclear factor-κB (NF-κB), but not NF-IL6 and STAT3, in the nucleus of Cygb-treated POA cells. These findings suggest that Cygb attenuates the secretion of IL-6 and TNF-α in LPS-stimulated POA primary cultures via inhibition of the NF-κB signaling pathway, indicating that this protein might play an important role in the control of neuroinflammation and fever.

show abstract

“…Both COX2 (Steiner et al, 2005) and mPGES1 (Engblom et al, 2003) were shown to be indispensable for LPS-induced fever. In addition to the PGE 2 locally produced in the brain, PGE 2 can also be secreted at the periphery predominantly by hepatic and pulmonary macrophages (Li et al, 2006;Simm et al, 2016;Steiner et al, 2006). This peripheral PGE 2 binds to albumin which protects it from enzymatic degradation during its journey to the brain via the bloodstream (Ivanov and Romanovsky, 2004).…”

Section: The Key Role Of Pgementioning

confidence: 99%

Behavioral fever in ectothermic vertebrates

Rakus

Ronsmans

Vanderplasschen

2017

Developmental & Comparative Immunology

View full text Add to dashboard Cite

a b s t r a c tFever is an evolutionary conserved defense mechanism which is present in both endothermic and ectothermic vertebrates. Ectotherms in response to infection can increase their body temperature by moving to warmer places. This process is known as behavioral fever. In this review, we summarize the current knowledge on the mechanisms of induction of fever in mammals. We further discuss the evolutionary conserved mechanisms existing between fever of mammals and behavioral fever of ectothermic vertebrates. Finally, the experimental evidences supporting an adaptive value of behavioral fever expressed by ectothermic vertebrates are summarized.

show abstract

Effects of prostaglandin E2 on cells cultured from the rat organum vasculosum laminae terminalis and median preoptic nucleus

Cited by 28 publications

References 59 publications

Rostral Ventrolateral Medulla EP3 Receptor Mediates the Sympathoexcitatory and Pressor Effects of Prostaglandin E₂ in Conscious Rats

Rostral Ventrolateral Medulla EP3 Receptor Mediates the Sympathoexcitatory and Pressor Effects of Prostaglandin E₂ in Conscious Rats

Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition

Behavioral fever in ectothermic vertebrates

Contact Info

Product

Resources

About

Effects of prostaglandin E2 on cells cultured from the rat organum vasculosum laminae terminalis and median preoptic nucleus

Cited by 28 publications

References 59 publications

Rostral Ventrolateral Medulla EP3 Receptor Mediates the Sympathoexcitatory and Pressor Effects of Prostaglandin E2 in Conscious Rats

Rostral Ventrolateral Medulla EP3 Receptor Mediates the Sympathoexcitatory and Pressor Effects of Prostaglandin E2 in Conscious Rats

Cytoglobin Attenuates Neuroinflammation in Lipopolysaccharide-Activated Primary Preoptic Area Cells via NF-κB Pathway Inhibition

Behavioral fever in ectothermic vertebrates

Contact Info

Product

Resources

About

Rostral Ventrolateral Medulla EP3 Receptor Mediates the Sympathoexcitatory and Pressor Effects of Prostaglandin E₂ in Conscious Rats

Rostral Ventrolateral Medulla EP3 Receptor Mediates the Sympathoexcitatory and Pressor Effects of Prostaglandin E₂ in Conscious Rats