“…is maintained after two consecutive doses of GHRH (1-29) (50, 100 and 200 pg/bolus), although the second GH responses are always significantly less than the first. In our and other's hands (Sartorio et al, 1986(Sartorio et al, , 1988Losa et al, 1986;Ghigo et al, 1989;Arosio et al, 1990) repeated GHRH administration (either at a dose/bolus of 1 pg/kg or at a standard dose of 50 or 80 pg i.v.) at intervals of 2 hours leads to a significant reduction in GH responsiveness (evaluated as net incremental area under the GH curve (GHnAUC) over 60 and 120 minutes after each bolus) to the second GHRH administration in normal adults.…”