Effects of propolis on cell growth and gene expression in HL-60 cells

Mishima, Satoshi; Narita, Yukio; Chikamatsu, Satomi; Inoh, Yoshikazu; Ohta, Shozo; Yoshida, Chie; Araki, Yasufumi; Akao, Yukihiro; Suzuki, Koji; Nozawa, Yoshinori

doi:10.1016/j.jep.2005.02.005

Cited by 48 publications

(39 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Inhibition of the production of nitric oxide and TNF has also been reported (Paulino et al, 2008). Further artepillin C was found to have strong antioxidant effects may be accounted for by additional effects of caffeoylquinic acid and other prenyl analogues (Nakajima et al, 2009;Mishima et al, 2005).…”

Section: Anti-inflammatory and Immunomodulatory Response To Isolated mentioning

confidence: 79%

Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review

Araújo

Libério

Guerra

et al. 2012

Rev. bras. farmacogn.

View full text Add to dashboard Cite

Many biological properties have been attributed to various types of propolis, including anti-inflammatory, antimicrobial, antioxidant, antitumor, wound healing, and immunomodulatory activities. This article reviewed studies published that investigated the anti-inflammatory activity of propolis of different origins and/ or its isolated components, focusing on the mechanisms of action underlying this activity and also addressing some aspects of immunomodulatory effects. The search was performed of the following databases: PubMed, Science Direct, HighWire Press, Scielo, Google Academics, Research Gate and ISI Web of Knowledgement. The anti-inflammatory activity was associated with propolis or compounds such as polyphenols (flavonoids, phenolic acids and their esters), terpenoids, steroids and amino acids. CAPE is the most studied compounds. The main mechanisms underlying the anti-inflammatory activity of propolis included the inhibition of cyclooxygenase and consequent inhibition of prostaglandin biosynthesis, free radical scavenging, inhibition of nitric oxide synthesis, reduction in the concentration of inflammatory cytokines and immunosuppressive activity. Propolis was found to exert an antiinflammatory activity in vivo and in vitro models of acute and chronic inflammation and others studies, indicating its promising potential as anti-inflammatory agent of natural origin and as a source of chemical compounds for the development of new drugs.

show abstract

Section: Anti-inflammatory and Immunomodulatory Response To Isolated mentioning

confidence: 79%

Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review

Araújo

Libério

Guerra

et al. 2012

Rev. bras. farmacogn.

View full text Add to dashboard Cite

show abstract

“…The effect of propolis on inhibition of different cell lines were reported for different cell lines such as K-562 (Aliyazicioglu et al 2005) HL-60 cells (Akao et al 2003;Mishima et al 2005), MCF-7 human breast cancer cells (Luo et al 2001) or some other leukemia cells (Hamblin 2006). There are number of reports indicating that some specific chemicals present in propolis such as caffeic acid phenethyl ester (CAPE) can inhibit growth of mutated cells without harming normal cells (Guarini et al 1992;Rao et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Effect of the water extracts of propolis on stimulation and inhibition of different cells

et al. 2007

View full text Add to dashboard Cite

The water extracts of propolis (WEP) could inhibit growth of different cell lines namely McCoy, HeLa, SP2/0, HEp-2, and BHK21 and stimulate growth of normal cell named human lymphocyte, rat kidney, rat liver, and rat spleen. In these experiments 1 and 2 mg of WEP were added to 1 ml RPMI media with 5% FCS. Cell counts and cell viability of propolis-treated and propolis-free cells were assessed by Trypan blue dye exclusion test and MTT assay. The results showed that in case of McCoy, HeLa, SP20, HEp-2, and BHK21 cell lines, the water extracts of propolis could inhibit cell growth as well as reduction on size of the cells. In contrast the same amount of WEP could stimulate growth of normal cells up to 60% with the same concentration used for cell lines. Thus our study indicates that although WEP consists only of the soluble part of propolis, it enables to inhibit different cell lines and increase growth of normal cells. This indicates also that WEP contains the specific compounds with bioactivity against cell lines. Although propolis contain different number of compounds it is clear that WEP has enough biological compounds useful for the treatment of some diseases, medical and related applications.

show abstract

“…These inhibitors were dissolved in DMSO. FACS analysis was performed as described previously (21). Fluorescein isothiocyanate (FITC)-anti-human CD38 antibody (eBioscience, San Diego, CA, USA) was also purchased.…”

Section: Identification Of the Transcription Start Sites Of Rck Mrnamentioning

confidence: 99%

Human DEAD-box/RNA unwindase rck/p54 contributes to maintenance of cell growth by affecting cell cycle in cultured cells

Akao¹,

Matsumoto²,

Ohguchi³

et al. 2006

Int J Oncol

Self Cite

View full text Add to dashboard Cite

Abstract. Understanding the control of gene expression in cancer cells requires defining the molecular and cellular basis of RNA metabolism compared with that in steady-state normal cells. Previously, we reported evidence that human RNA structure-modifying unwindase rck/p54, a member of the DEAD-box family, was highly expressed in most of the malignant cell lines tested and that this expression was linked to malignant transformation. Here, we show that rck/p54 positively affects cell growth, probably by modulating the gene expression at the translational level in cultured cells. In cell growth and differentiation induced by external stimuli, the level of rck/p54 expression was up-regulated during cell proliferation and down-regulated during differentiation. The down-regulation of rck/p54 in HeLa cells by RNAi induced cell growth inhibition through cell cycle arrest at S phase. Immunoprecipitation using anti-rck/p54 antibody in HeLa cells demonstrated the co-precipitation of rck/p54 with eIF4E, which is well-known to bind to the 5'cap-structure, resulting in initiation of translation. These data suggest that rck/p54 contributes to cell growth possibly by modulating translationinitiation control of the genes involved in the cell proliferation, which is a newly defined mechanism leading to carcinogenesis.

show abstract

Effects of propolis on cell growth and gene expression in HL-60 cells

Cited by 48 publications

References 15 publications

Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review

Mechanisms of action underlying the anti-inflammatory and immunomodulatory effects of propolis: a brief review

Effect of the water extracts of propolis on stimulation and inhibition of different cells

Human DEAD-box/RNA unwindase rck/p54 contributes to maintenance of cell growth by affecting cell cycle in cultured cells

Contact Info

Product

Resources

About