Effects of prolonged exercise and recovery on sarcoplasmic reticulum Ca<sup>2+</sup> cycling properties in rat muscle homogenates

Schertzer, Jonathan D.; Green, H J; Fowles, Jonathon R.; Duhamel, Todd A.; Tupling, A. Russell

doi:10.1046/j.0001-6772.2003.01227.x

Cited by 17 publications

(28 citation statements)

References 47 publications

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“…Ca 2ϩ -induced SERCA activity in enriched SR membranes was analyzed according to the methods of Leberer et al (14), described previously (24). Ca 2ϩ -induced SERCA activity in crude muscle homogenates was analyzed with the use of modifications according to Simonides and van Hardeveld (29), as described previously (25). The reaction buffer for crude muscle homogenates (with SR vesicles in parentheses) contained, in mM, 200 (100) KCl, 20 HEPES, 15 (10) MgCl 2, 10 NaN3, 10 phosphoenolpyruvate (PEP), 5 ATP, and 1 EGTA.…”

Section: Methodsmentioning

confidence: 99%

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β₂-Agonist administration increases sarcoplasmic reticulum Ca²⁺-ATPase activity in aged rat skeletal muscle

Schertzer¹,

Plant²,

Ryall³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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-Agonist administration increases sarcoplasmic reticulum Ca 2ϩ -ATPase activity in aged rat skeletal muscle. Am J Physiol Endocrinol Metab 288: E526 -E533, 2005. First published October 12, 2004 doi:10.1152/ ajpendo.00399.2004.-Aging is associated with a slowing of skeletal muscle contractile properties, including a decreased rate of relaxation. In rats, the age-related decrease in the maximal rate of relaxation is reversed after 4-wk administration with the ␤ 2-adrenoceptor agonist (␤ 2-agonist) fenoterol. Given the critical role of the sarcoplasmic reticulum (SR) in regulating intracellular Ca 2ϩ transients and ultimately the time course of muscle contraction and relaxation, we tested the hypothesis that the mechanisms of action of fenoterol are mediated by alterations in SR proteins. Sarcoendoplasmic reticulum Ca 2ϩ -ATPase (SERCA) kinetic properties were assessed in muscle homogenates and enriched SR membranes isolated from the red (RG) and white (WG) portions of the gastrocnemius muscle in adult (16 mo) and aged (28 mo) F344 rats that had been administered fenoterol for 4 wk (1.4 mg/kg/day ip, in saline) or vehicle only. Aging was associated with a 29% decrease in the maximal activity (V max) of SERCA in the RG but not in the WG muscles. Fenoterol treatment increased the V max of SERCA and SERCA1 protein levels in RG and WG. In the RG, fenoterol administration reversed an age-related selective nitration of the SERCA2a isoform. Our findings demonstrate that the mechanisms underlying age-related changes in contractile properties are fiber type dependent, whereas the effects of fenoterol administration are independent of age and fiber type.aging; calcium; ␤-adrenoceptor; contractility SARCOPENIA, THE PROGRESSIVE LOSS of skeletal muscle mass with advancing age, is associated with a decline in muscle strength that leads to a loss of functional independence and a reduced quality of life (17). Aging is also associated with a slowing of the time course of skeletal muscle contraction and relaxation (15). These changes can reduce the accuracy and precision of movements, impair the ability to perform simple tasks, and increase the risk of sudden falls and related injuries. Ideally, therapies to prevent or reverse the age-related changes in skeletal muscle should also target the slowing of contraction and relaxation, in addition to preserving or increasing muscle mass and strength.Neurogenic factors, such as motor unit remodeling and denervation, have been implicated in the age-related changes to skeletal muscle (11). However, myogenic factors are also involved, since the slowing of contraction and relaxation occurs before the onset of muscle wasting (18,20,21). Intrinsic changes to skeletal muscle fibers appear to be involved in the deterioration of function with age. Specifically, age-related changes in the proteins regulating Ca 2ϩ handling during excitation-contraction coupling and relaxation have been described (15).Relaxation of skeletal muscle is thought to involve at least three processes: dissociation of Ca 2...

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Section: Methodsmentioning

confidence: 99%

“…The rats were killed by the opening of the thoracic cavity and immediate cardiac excision. Crude muscle homogenates were prepared, as described previously (24,25). SR vesicles were prepared, utilizing a combination of two SR isolation protocols (6, 9), as described previously (24).…”

Section: Methodsmentioning

confidence: 99%

β₂-Agonist administration increases sarcoplasmic reticulum Ca²⁺-ATPase activity in aged rat skeletal muscle

Schertzer¹,

Plant²,

Ryall³

et al. 2005

American Journal of Physiology-Endocrinology and Metabolism

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“…This occurred because of errors in the software used to calculate [Ca 2ϩ ]f in conjunction with a dilution error. The procedures for measuring Ca 2ϩ -ATPase activity in our laboratory have been described in detail (45,50). Essentially the same procedures were employed as for measuring Ca 2ϩ uptake.…”

Section: Participantsmentioning

confidence: 99%

“…The SR Ca 2ϩ -ATPase isoforms (SERCA1 and SERCA2a) were measured with the use of sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and immunoblotting with the primary monoclonal antibodies specific for human SERCA1 (MA3-912; Affinity Bioreagents) and SERCA2a (MA3-919; Affinity Bioreagents) as detailed previously (45). A 7.5% polyacrylamide SDS gel and a 3.75% stacking gel were employed.…”

Section: Participantsmentioning

confidence: 99%

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Comparative effects of a low-carbohydrate diet and exercise plus a low-carbohydrate diet on muscle sarcoplasmic reticulum responses in males

Duhamel

Green²,

Perco³

et al. 2006

American Journal of Physiology-Cell Physiology

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ployed a glycogen-depleting session of exercise followed by a lowcarbohydrate (CHO) diet to investigate modifications that occur in muscle sarcoplasmic reticulum (SR) Ca 2ϩ -cycling properties compared with low-CHO diet alone. SR properties were assessed in nine untrained males [peak aerobic power (V O2 peak) ϭ 43.6 Ϯ 2.6 (SE) ml ⅐ kg Ϫ1 ⅐ min Ϫ1 ] during prolonged cycle exercise to fatigue performed at ϳ58% V O2 peak after 4 days of low-CHO diet (Lo CHO) and after glycogen-depleting exercise plus 4 days of low-CHO (ExϩLo CHO). Compared with Lo CHO, ExϩLo CHO resulted in 12% lower (P Ͻ 0.05) resting maximal Ca 2ϩ -ATPase activity (Vmax ϭ 174 Ϯ 12 vs. 153 Ϯ 10 mol ⅐ g protein Ϫ1 ⅐ min Ϫ1 ) and smaller reduction in Vmax induced during exercise. A similar effect was observed for Ca 2ϩ uptake. The Hill coefficient, defined as slope of the relationship between cytosolic free Ca 2ϩ concentration and Ca 2ϩ -ATPase activity, was higher (P Ͻ 0.05) at rest (2.07 Ϯ 0.15 vs. 1.90 Ϯ 0.10) with ExϩLo CHO, an effect that persisted throughout the exercise. The coupling ratio, defined as the ratio of Ca 2ϩ uptake to Vmax, was 23-30% elevated (P Ͻ 0.05) at rest and during the first 60 min of exercise with ExϩLo CHO. The ϳ27 and 34% reductions (P Ͻ 0.05) in phase 1 and phase 2 Ca 2ϩ release, respectively, observed during exercise with Lo CHO were not altered by ExϩLo CHO. These results indicate that when prolonged exercise precedes a short-term Lo CHO diet, Ca 2ϩ sequestration properties and efficiency are improved compared with those during Lo CHO alone. calcium cycling; vastus lateralis; contractile activity; glycogen; phosphorylation potential ADEQUATE RESERVES of the endogenous muscle carbohydrate glycogen are recognized as essential for sustaining contractile activity (2,7,20). Intuitively, the most obvious mechanism for this effect would appear to relate to its role as a fuel for oxidative phosphorylation and glycolysis, the metabolic pathways involved in regenerating the ATP necessary to supply the energy needs of the active muscle. According to this theory, the depletion of muscle glycogen would be expected to compromise the flux rate through these metabolic pathways resulting in an energy crisis and an inability to supply the ATP require-

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Maximal voluntary contraction force, SR function and glycogen resynthesis during the first 72 h after a high-level competitive soccer game

et al. 2011

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The aim of this study was to examine maximal voluntary knee-extensor contraction force (MVC force), sarcoplasmic reticulum (SR) function and muscle glycogen levels in the days after a high-level soccer game when players ingested an optimised diet. Seven high-level male soccer players had a vastus lateralis muscle biopsy and a blood sample collected in a control situation and at 0, 24, 48 and 72 h after a competitive soccer game. MVC force, SR function, muscle glycogen, muscle soreness and plasma myoglobin were measured. MVC force sustained over 1 s was 11 and 10% lower (P < 0.05) after 0 and 24 h, respectively, compared with control. The rate of SR Ca(2+) uptake at 800 nM [Ca(2+)](free) was lower (P < 0.05) after 0 h (2.5 μmol Ca(2+) g prot(-1) min(-1)) than for all other time points (24 h: 5.1 μmol Ca(2+) g prot(-1) min(-1)). However, SR Ca(2+) release rate was not affected. Plasma myoglobin was sixfold higher (P < 0.05) immediately after the game, but normalised 24 h after the game. Quadriceps muscle soreness (0-10 VAS-scale) was higher (P < 0.05) after 0 h (3.6), 24 h (1.8), 48 h (1.1) and 72 h (1.4) compared with control (0.1). Muscle glycogen was 57 and 27% lower (P < 0.001) 0 and 24 h after the game compared with control (193 and 328 vs. 449 mmol kg d w(-1)). In conclusion, maximal voluntary contraction force and SR Ca(2+) uptake were impaired and muscle soreness was elevated after a high-level soccer game, with faster recovery of SR function in comparison with MVC force, soreness and muscle glycogen.

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Effects of prolonged exercise and recovery on sarcoplasmic reticulum Ca²⁺ cycling properties in rat muscle homogenates

Abstract: It is concluded that in crude homogenates, SR Ca2+-uptake and Ca2+-ATPase activity are elevated in recovery following prolonged running and that the elevation in these properties is more pronounced during passive compared with active recovery.

Cited by 17 publications

References 47 publications

β₂-Agonist administration increases sarcoplasmic reticulum Ca²⁺-ATPase activity in aged rat skeletal muscle

β₂-Agonist administration increases sarcoplasmic reticulum Ca²⁺-ATPase activity in aged rat skeletal muscle

Comparative effects of a low-carbohydrate diet and exercise plus a low-carbohydrate diet on muscle sarcoplasmic reticulum responses in males

Maximal voluntary contraction force, SR function and glycogen resynthesis during the first 72 h after a high-level competitive soccer game

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Effects of prolonged exercise and recovery on sarcoplasmic reticulum Ca2+ cycling properties in rat muscle homogenates

Abstract: It is concluded that in crude homogenates, SR Ca2+-uptake and Ca2+-ATPase activity are elevated in recovery following prolonged running and that the elevation in these properties is more pronounced during passive compared with active recovery.

Cited by 17 publications

References 47 publications

β2-Agonist administration increases sarcoplasmic reticulum Ca2+-ATPase activity in aged rat skeletal muscle

β2-Agonist administration increases sarcoplasmic reticulum Ca2+-ATPase activity in aged rat skeletal muscle

Comparative effects of a low-carbohydrate diet and exercise plus a low-carbohydrate diet on muscle sarcoplasmic reticulum responses in males

Maximal voluntary contraction force, SR function and glycogen resynthesis during the first 72 h after a high-level competitive soccer game

Contact Info

Product

Resources

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Effects of prolonged exercise and recovery on sarcoplasmic reticulum Ca²⁺ cycling properties in rat muscle homogenates

β₂-Agonist administration increases sarcoplasmic reticulum Ca²⁺-ATPase activity in aged rat skeletal muscle

β₂-Agonist administration increases sarcoplasmic reticulum Ca²⁺-ATPase activity in aged rat skeletal muscle