Effects of probiotics on the severity of experimental acute pancreatitis

Abstract

Objective: This study was designed to evaluate the effects of probiotics on the severity of experimental acute pancreatitis. Design: Experimental study. Setting: Experiments were done in a laboratory at Haydarpasa Numune Teaching and Research Hospital. Subjects:A total of 50 Wistar rats were randomly divided into five groups. Interventions: Group 1 was control group. Group 2 received an intraperitoneal injection of a 20% solution in 0.15 mol/l NaCL. Group 3 was injected NaCL and fed with probiotics. Acute pancreatitis was induced in rats by intrperitoneal injection of L-Arginine in groups 4 and 5. The rats in group 5 were treated with probiotics. The pancreas was removed for histologic examination. Evaluation of the pathologic changes was done by a new combined histopathologic grading scale. Results: The mean scores of fibrosis, acinar cell loss, oedema, parenchymal necrosis, mononuclear cells infiltration, polymorphonuclear leucocytes infiltration, ductal damage and atypical reactive regeneration in group 5 were significantly lower than group 4. Conclusions: We demonstrated that enteral feedings with added probiotics can reduce the severity of acute pancreatitis. Sponsorship: None.

Early enteral nutrition in acute pancreatitis—benefits and limitations

“…They found that the histopathologic scores of acute necrotizing pancreatitis were significantly lower. A study published by Muftuoglu et al [55] confirmed that enteral feeding completed with probiotics reduced the severity of acute experimental pancreatitis based on histological analysis. Another paper by van Minnen et al [56] demonstrated that probiotics reduced duodenal bacterial overgrowth of potential pathogens, resulting in reduced bacterial translocation to extra-intestinal sites, including the pancreas.…”

Effect of Enteral Nutrition and Synbiotics on Bacterial Infection Rates After Pylorus-preserving Pancreatoduodenectomy

“…Two recent studies of rats analyzed the impact of pretreatment with synbiotics on the severity of experimental acute pancreatitis. Pretreatment with synbiotics reduced the severity of histopathologic findings 27 and reversed the pancreatitis-induced increase in endotoxemia and transaminase levels 28 compared with saline or metronidazole.…”

Evidence-based use of enteral nutrition in acute pancreatitis

“…The excellent results of using probiotics in experimental pancreatitis models were promising initially [55][56][57][58][59]. Mangiante et al significantly reduced infected necrosis rate using Lactobacillus plantarum [55], and a study by van Minnen et al from Utrecht demonstrated that probiotics reduced significantly even the late-phase mortality [58].…”