Effects of Probiotics on Growth, Survival, Water Quality and Disease Resistance of Red Hybrid Tilapia (Oreochromis spp.) Fingerlings in a Biofloc System

Abstract

Biofloc technology has shown positive effects in aquaculture, especially on the growth performance of cultured animals. The aims of this study were to evaluate the effects of adding different probiotic strains in a biofloc system on the growth performance and disease resistance of red hybrid tilapia (Oreochromis spp.). Three different probiotics (Lysinibacillus fusiformis SPS11, Bacillus amyloliquefaciens L9, and Enterococcus hirae LAB3), commercial probiotics (MG1) and a mixed probiotics (MP) combining all three strains were used in this study. The in vitro assay results showed that the mixed probiotic (MP) was able to inhibit the growth of Streptococcus agalactiae and Streptococcus iniae significantly compared to the single and commercial probiotic. The efficacy of MP was further tested in in vivo tilapia culture challenged with S. agalactiae. The best specific growth rate (3.73 ± 0.23% day−1) and feed conversion ratio (0.76 ± 0.04) were recorded in the group of biofloc with addition of MP. After being challenged with S. agalactiae, the group of biofloc with MP had significantly higher survival (83 ± 1.43%) compared to the other groups. Furthermore, the nitrogen concentration (NO2-N and NH4-N) was significantly lower in all the biofloc groups compared to the control. Hence, the addition of probiotics was able to provide beneficial effects to red hybrid tilapia culture in the biofloc system.

Probiotics enhance resistance to Streptococcus iniae in Nile tilapia (Oreochromis niloticus) reared in biofloc systems

“…Biofloc systems may protect against bacterial infections, however, in this study fish fed with the commercial feed only in the biofloc did not show considerable protection against S. iniae , and when supplemented with probiotic treatments, the protection was increased. The same trend of probiotic effectiveness against tilapia pathogens following biofloc rearing has been reported in other recent studies (Mohammadi et al, 2021; Zabidi et al, 2021). One challenge for comparisons across trials is the actual microbial communities within the biofloc system and their inherent interactions with the tank system and the fish.…”

Improvement of water quality with probiotics inclusion during simulated transport of Yellowfin Seabream Acanthopagrus latus larvae

“…The most common lactic acid bacteria used in finfish culture include Carnobacterium, Enterococcus, Pediococcus, Lactobacillus, Lactococcus, and Leuconostoc genera (Merrifield and Ringo 2014). Zabidi et al (2021) reported that a probiotic mixture was able to prevent an increase in Streptococcus iniae and Streptococcus agalactiae. Individual bacterial species have specific limitations in nitrogen and phosphorus removal and pathogen control (Li et al 2022).…”

“…Zabidi et al. (2021) reported that a probiotic mixture was able to prevent an increase in Streptococcus iniae and Streptococcus agalactiae . Individual bacterial species have specific limitations in nitrogen and phosphorus removal and pathogen control (Li et al.…”

Systematic literature review identifying bacterial constituents in the core intestinal microbiome of rainbow trout (Oncorhynchus mykiss)

“…To provide a viable alternative to prophylactic antibiotic administration, which is still done in some regions outside of the United States, an increasing amount of research has been conducted on the efficacy of probiotics (live microbes) and prebiotics (compounds metabolised by microbes) in promoting a healthy microbiome. For instance, these dietary supplements have the capability to support healthy fish physiology and microbiomes while also inhibiting pathogen proliferation (Al‐Hisnawi et al., 2019; Dawood et al., 2020; Giorgia et al., 2018; Hooshyar et al., 2020; Mora‐Sánchez et al., 2020; Park et al., 2017; Zabidi et al., 2021). Dietary changes especially affect the inhabitants of the gastrointestinal tract.…”