Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

Jiang, Yan; Zhang, Zheng; Wang, Yingeng; Jing, Yayun; Liao, Meijie; Rong, Xiaojun; Li, Bin; Chen, Guiping; Zhang, Hesen

doi:10.1007/s00343-018-7049-1

Cited by 9 publications

(7 citation statements)

References 52 publications

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“…amyloliquefaciens could not be detected by real-time Polymerase Chain Reaction (RT-PCR). Furthermore, the OTUs representing the genus Bacillus were not dominant in the live feed [71] as well as in guts of the turbot larvae and juveniles. Hence, we inferred that the abundance of B .…”

Section: Discussionmentioning

confidence: 99%

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Responses of microbial community structure in turbot (Scophthalmus maximus) larval intestine to the regulation of probiotic introduced through live feed

et al. 2019

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Various bacteria that adhere to the gut are important for the health of fish. Regulating the microbial community in a desirable direction may be beneficial in aquaculture for preventing and controlling the diseases caused by pathogenic microbes. In this study, we investigated the changes in the microflora in the intestinal tracts of turbot ( Scophthalmus maximus ) larvae after introducing a probiotic ( Bacillus amyloliquefaciens ) after the first feed. B . amyloliquefaciens was added as part of a nutrient enrichment system in live feed ( Branchionus plicatilis or Artemia sinica ), so it passed into the intestinal tracts of the newly hatched turbot larvae. The turbot larvae were fed on live feed containing B . amyloliquefaciens in the experimental group, whereas live feed without the probiotic was provided to larvae in the control group. The total bacterial genomic DNA in the larval guts was extracted and sequenced with an Illumina HiSeq PE250 system. According to the sequencing results, the abundances of microbial species and the microflora diversity were lower in the intestines in the experimental group than the control. Throughout development, the microflora structure in the intestines was mainly constructed before the first feed and the composition of the dominant operational taxonomic units (OTUs) was stable, where the abundances of OTU8, OTU124, OTU150, OTU107, and OTU17 were always high. Compared with the control, the structures of the microflora in the intestines were similar on different days during the development and the growth of larvae in the experimental group. However, the similarity of the microflora structure between different treatments was low on the same day. Furthermore, the mean proportion of common OTUs was only 74.7% in different treatments on each day, which indicates that the introduction of B . amyloliquefaciens in the live feed changed the microflora structure in the intestine. During the early development stage (days 3–30), the average abundance of Pseudomonas was reduced by 0.8% whereas that of Lactococcus increased by 3.5% in the experimental group. Pseudomonas spp. are considered potentially pathogenic bacteria but there is no direct evidence for the pathogenicity of Lactococcus in turbot. Moreover, several Lactococcus species are regarded as probiotics in aquaculture. Therefore, the use of B . amyloliquefaciens could be beneficial for optimizing the microbial community structure in the intestines of turbot larvae, which may explain the probiotic effect of B . amyloliquefaciens . This study provides a theoretical basis for the biological regulation...

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Section: Discussionmentioning

confidence: 99%

“…However, the structure of the microbiota in live feed was clearly changed by the addition of B . amyloliquefaciens [71]. Similarly, the structures of microbiota in turbot larval intestines were also influenced.…”

Section: Discussionmentioning

confidence: 99%

Responses of microbial community structure in turbot (Scophthalmus maximus) larval intestine to the regulation of probiotic introduced through live feed

et al. 2019

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“…Addition of probionts, either directly to the water in rearing tanks or via live food influence the species-composition of microflora (Makridis et al, 2000). Jiang et al (2018) observed the increase in the abundance of microbial species and diversity of microflora in rotifers and Artemia sinica when probiont was added. Despite the changes in the structure of microbiota, the abundance of probiont was relatively low in live feed (Jiang et al, 2019).…”

Section: Resultsmentioning

confidence: 93%

“…By taking advantage of primitive feeding characteristics, it is possible to manipulate the nutritional value of Moina through enrichment process (Das et al, 2007;Singh et al, 2019). Additionally, the abundance of microbial species and diversity of microflora in live feed can be enhanced with probiotics (Jiang et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

Enrichment of freshwater zooplankton Moina micrura with probiotics isolated from microalgae

Samat¹,

Yusoff²,

Chen³

et al. 2020

JEB

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“…Several studies also demonstrated that probiotic feeding in early life stage of fish (e.g. larvae) is profitable due to the fact that disease load is low in the later stage (D'Alvise et al., 2012; Jiang et al., 2018; Sayes et al., 2018). However, the protection of hatchlings or larvae is the most challenging part since delivery of probiotics during early stage is quite difficult.…”

Section: Discussionmentioning

confidence: 99%

Assessing the effect of probiotics on tilapia lake virus‐infected tilapia: Transmission and immune response

Yang

Yamkasem

Surachetpong

et al. 2022

Journal of Fish Diseases

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Probiotics have been used to alleviate disease transmission in aquaculture. However, there are limited studies on probiotic use in modulating tilapia lake virus (TiLV). We assessed commercially available probiotic supplements used in TiLV‐infected tilapia and performed mortality and cohabitation assays. We developed a mechanistic approach to predict dose–response interactions of probiotic effects on mortality and immune gene response. We used a susceptible–infected–mortality disease model to assess key epidemiological parameters such as transmission rate and basic reproduction number (R0) based on our viral load dynamic data. We found that the most marked benefits of probiotics are significantly associated with immune system enhancements (~30%) and reductions in disease transmission (~80%) and R0 (~70%) in tilapia populations, resulting in a higher tolerance of farming densities (~400 fold) in aquaculture. These findings provide early insights as to how probiotic use‐related factors may influence TiLV transmission and the immune responses in TiLV‐infected tilapia. Our study facilitates understanding the mode of action of probiotics in disease containment and predicting better probiotic dosages in diet and supplements to achieve the optimal culturing conditions. Overall, our analysis assures that further study of rationally designed and targeted probiotics, or mechanistic modelling is warranted on the basis of promising early data of this approach.

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Effects of probiotic on microfloral structure of live feed used in larval breeding of turbot Scophthalmus maximus

Cited by 9 publications

References 52 publications

Responses of microbial community structure in turbot (Scophthalmus maximus) larval intestine to the regulation of probiotic introduced through live feed

Responses of microbial community structure in turbot (Scophthalmus maximus) larval intestine to the regulation of probiotic introduced through live feed

Enrichment of freshwater zooplankton Moina micrura with probiotics isolated from microalgae

Assessing the effect of probiotics on tilapia lake virus‐infected tilapia: Transmission and immune response

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