Effects of Probiotic NVP-1704 on Mental Health and Sleep in Healthy Adults: An 8-Week Randomized, Double-Blind, Placebo-Controlled Trial

Abstract

The human gut microbiome is closely linked to mental health and sleep. We aimed to verify the efficacy and safety of probiotic NVP-1704, a mixture of Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98, in improving stress, depression, anxiety, and sleep disturbances, along with the measurement of some blood biomarkers. A total of 156 healthy adults with subclinical symptoms of depression, anxiety, and insomnia were retrospectively registered and randomly assigned to receive either NVP-1704 (n = 78) or a placebo (n = 78) for eight weeks. Participants completed the Stress Response Inventory, Beck’s Depression and Anxiety Inventory, Pittsburg Sleep Quality Index, and Insomnia Severity Index at baseline, at four and eight weeks of treatment. Pre- and post-treatment blood tests for biomarkers were conducted. After intervention, gut microbiota composition was quantified by pyrosequencing the bacterial 16S rRNA gene. The NVP-1704 group had a more significant reduction in depressive symptoms at four and eight weeks of treatment, and anxiety symptoms at four weeks compared to the placebo group. Those receiving NVP-1704 also experienced an improvement in sleep quality. NVP-1704 treatment led to a decrease in serum interleukin-6 levels. Furthermore, NVP-1704 increased Bifidobacteriaceae and Lactobacillacea, whereas it decreased Enterobacteriaceae in the gut microbiota composition. Our findings suggest that probiotic NVP-1704 could be beneficial for mental health and sleep.

The Alleviation of Gut Microbiota-Induced Depression and Colitis in Mice by Anti-Inflammatory Probiotics NK151, NK173, and NK175

“…Lactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 mitigate stress-induced depression and colitis in mice by the attenuation of gut dysbiosis [ 16 , 20 ]. NVP1704, a combination of NK33 and NK98, alleviates depressive and anxious symptoms in individuals with anxiety, depression, and insomnia [ 24 ]. In addition, anti-inflammatory drugs mitigate gut inflammation and depression [ 25 ].…”

Probiotics, prebiotics and postbiotics for better sleep quality: a narrative review

“…As such, a pilot study in patients with chronic primary insomnia suggested that the consumption of L. plantarum PS128 could reduce the events of waking up at NREM3 sleep, compared with the placebo group (Ho et al, 2021). Another study, in stressed adults with symptoms of depression or anxiety, reported that Limosilactobacillus reuteri NK33 and Bifidobacterium adolescentis NK98 tended to reduce the overall PSQI scores after eight weeks of consumption, compared with the placebo group (P=0.068) (Lee et al, 2021). In a singlearm study where highly stressed IT workers consumed L. plantarum PS128 daily for eight weeks, both stress and insomnia symptoms were reduced at week eight compared with baseline measurements (Wu et al, 2021).…”

Using Microbiome-Based Approaches to Deprogram Chronic Disorders and Extend the Healthspan following Adverse Childhood Experiences

“…[145] A probiotic mix improved sleep quality. [146] Gut microbiome status including altered metabolism affects sleep. [147] Probiotic supplementation improved sleep among postoperative cancer patients.…”