Effects of Probiotic Complex on Performance, Blood Biochemical and Immune Parameters, Digestive Enzyme Activity, Fecal Microbial Population and Noxious Gas Emission in Broiler Chicks

Abstract

This study examined the effects of a probiotic complex (PC) containing Lactobacillus plantarum, Bacillus subtilis, and Saccharomyces cerevisiae on growth performance, organ weight, immune parameters, fecal microbial count, and noxious odor in broiler chicks. A total of 216 birds (4-day-old) were fed a basal diet (CON) and a diet supplemented with 0.25% (PC1) and 0.5% (PC2) of PC until 35 days of age. No difference in body weight, feed intake, and FCR was observed among the groups. The intestinal mucosal weight of the PC1 group was greater than that of the CON group without affecting weights of the other organs. Intestinal secretory immunoglobulin A (sIgA) levels in the PC2 group increased significantly (P<0.05) compared with that in the CON group. The PC2 group also had a strong tendency for elevated blood sIgA levels. Dietary PC did not affect the level of interleukin-1β in the blood and mucosal tissues or alter maltase, sucrase, and leucine aminopeptidase activities in the intestinal mucosa. The PC2 group had higher colony-forming units (cfu) for L. plantarum and S. cerevisiae, but lower cfu for E. coli than those in the CON group. Compared to the CON diet, the PC2 diet resulted in a decreased H 2 S concentration and a tendency toward decreased CH 3 SH concentration. In conclusion, a 0.5% PC diet showed increased sIgA and desirable microbial population, and decreased noxious odor in the feces, suggesting that PC could be applied as an environmentally friendly feed additive in broiler chicks.

Effects of Probiotics on Antioxidant Biochemical Parameters and Antioxidant Enzymes in the Blood, Intestinal Mucosal Tissues and Liver of Broiler Chicks under High Ambient Temperature Conditions

“…생균 제의 주요 작용 기전은 소화관에서 유익균의 정착을 도와 소장 점막 세포의 구조적 안정화 및 병원성균을 억제하여 면역 작용을 높이는 것으로 알려져 있다 (Rajput et al, 2013;Lee et al, 2014). 최근 연구 결과 복합생균제 급여 시 소장 점막 세포에서 병원성 미생물 방어에 중요한 분비형 면역 글로불린(sIgA) 수준이 증가하고 유익균총이 증가하는 것으 로 보고되었다 (Kim et al, 2020).…”