Effects of previous physical training on adriamycin nephropathy and its relationship with endothelial lesions and angiogenesis in the renal cortex

Faleiros, Camila M; Francescato, Heloı́sa Della Coletta; Papoti, Marcelo; Chaves, Lucas; Silva, Cleonice G.A.; Costa, R. S.; Coimbra, Terezila Machado

doi:10.1016/j.lfs.2016.11.014

Cited by 28 publications

(28 citation statements)

References 69 publications

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“…First, the rats were selected based on their ability to run on a treadmill (EPR model; Gesan, São Paulo, Brazil) with 0% incline [13]. Rats were subjected to daily sessions of increasing duration (5-20 min) and intensity (running velocity: 5-20 m/min) over 5 days.…”

Section: Methodsmentioning

confidence: 99%

“…Intensity and duration varied according to two grades of aerobic training/resistance: easy (continuous exercise for 60 min using 50% of MLSS intensity) and moderate (continuous running lasting 30 min using 100% of MLSS intensity) [13, 28]. …”

Section: Methodsmentioning

confidence: 99%

“…The membrane-bound antibody was detected with Supersignal West Pico Chemiluminescent substrate (Pierce, Rockford, IL) and captured on X-ray film. The intensity of the identified lanes was quantified by densitometry with ImageJ NIH image software and was reported in arbitrary units [13]. …”

Section: Methodsmentioning

confidence: 99%

“…Regular moderate-intensity exercise training before induction of diabetes mellitus improved metabolic control, renal function, and structural changes, and reduced the expression of transforming growth factor (TGF)-β in renal tissue, which was associated with decreased fibronectin expression and a renoprotective effect [12]. We also observed in a recent study that previous training can reduce renal endothelial lesions and improve angiogenesis in Adriamycin-treated rats [13]. …”

Section: Introductionmentioning

confidence: 99%

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Previous Exercise Effects in Cisplatin-Induced Renal Lesions in Rats

Francescato

Almeida

Reis

et al. 2018

Kidney Blood Press Res

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Background/Aims: Physical training has beneficial effects on endothelial function and can influence the regeneration of the endothelial cell. We investigated the effect of physical training on cisplatin (CP)-induced acute kidney injury and assessed the impact of training on endothelial structure and function, and on the inflammatory processes in rats. Methods: We injected male Wistar rats subjected to previous physical training in treadmill running (trained, TR) or not (sedentary, SED) with CP (5 mg/kg) (TR+CP and SED+CP groups, respectively). Five days after the injections, blood and urine samples were collected to evaluate renal function and kidneys were harvested for morphological, immunohistochemical, enzyme-linked immunosorbent assay, and analysis of nitric oxide (NO) levels. Results: Rats treated with CP showed increased levels of plasma creatinine and sodium and potassium fractional excretion. These alterations were associated with increase in tubulointerstitial lesions and macrophage number, reduction of endothelial cells, and increased VEGF, vimentin, and α-smooth muscle actin expression in the outer renal medulla in the SED+CP group. We also found increased levels of renal IL-1β and increased excretion of monocyte chemoattractant protein-1 and transforming growth factor-β compared with controls. These changes were milder in trained rats, associated with increased levels of renal tissue NO, and increased expression of p-eNOS and stromal cell-derived factor-1α (a chemokine involved in kidney repair) in the kidneys of CP-injected trained rats. Conclusions: The protective effect of previous training in CP-treated rats was associated with reduced endothelial cell lesions and increased renal production of NO in trained rats.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Previous Exercise Effects in Cisplatin-Induced Renal Lesions in Rats

Francescato

Almeida

Reis

et al. 2018

Kidney Blood Press Res

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“…A previous study by our group demonstrated that MHCD attenuated renal fibrosis by inhibiting excessive autophagy and upregulating basal autophagy in rats with Doxorubicin-induced nephropathy (14), which is an experimental model of progressive kidney disease (15,16). In the present study, Doxorubicin-induced nephrotic rats with proteinuria were used as an experimental model.…”

Section: Introductionmentioning

confidence: 99%

Modified Huangqi Chifeng decoction inhibits excessive autophagy to protect against Doxorubicin‑induced nephrotic syndrome in rats via the PI3K/mTOR signaling pathway

Yang

et al. 2018

Exp Ther Med

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The aim of the present study was to investigate whether modified Huangqi Chifeng decoction (MHCD) could be an effective treatment against Doxorubicin-induced nephrosis in rats and whether it regulates autophagy via the phosphoinositide-3 kinase/mammalian target of rapamycin (PI3K/mTOR) signaling pathway. A total of 40 male Sprague-Dawley rats were randomly divided into blank, model, telmisartan and MHCD groups. The rat model of nephrosis was induced by intragastric administration of Doxorubicin for 8 weeks. Rats were housed in metabolic cages and urine was collected once every 2 weeks to measure 24-h protein levels. Blood samples were obtained from the abdominal aorta and levels of albumin (ALB), total cholesterol (TCH), triacylglyceride (TG) and serum creatinine (Scr) were assessed. Renal pathological changes were examined using hematoxylin-eosin, Masson's trichome and periodic acid-Schiff staining. Podocytes and autophagosomes were observed using an electron microscope. The expression and distribution of microtubule-associated proteins 1A/1B light chain 3B (LC3), LC3-I, LC3-II, beclin-1, PI3K and mTOR were determined using immunohistochemistry and western blotting. At weeks 6 and 8, 24-h proteinuria significantly decreased in the MHCD group compared with the model group (P<0.05). Compared with the model group, the MHCD group exhibited significantly reduced levels of TG, TCH and Scr, as well as significantly increased ALB levels (P<0.05). MHCD was demonstrated to prevent glomerular and podocyte injury. The number of autophagosomes was significantly decreased and the expression of beclin-1, LC3, LC3-I and LC3-II was inhibited following MHCD treatment compared with the model group (P<0.05). MHCD treatment significantly increased the expression of PI3K and mTOR in Doxorubicin nephrotic rats compared with the model group (P<0.05). In conclusion, MHCD was demonstrated to ameliorate proteinuria and protect against glomerular and podocyte injury by inhibiting excessive autophagy via the PI3K/mTOR signaling pathway.

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Benefits of high-intensity interval training compared to continuous training to reduce apoptotic markers in female rats with cisplatin nephrotoxicity – possible modulatory role of IL-11

et al. 2023

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Effects of previous physical training on adriamycin nephropathy and its relationship with endothelial lesions and angiogenesis in the renal cortex

Abstract: Physical training prior to ADR injection reduced the renal damage induced by this drug. This effect was related to angiogenesis and reduction in the endothelial lesions and inflammatory process in the renal cortex of these animals.

Cited by 28 publications

References 69 publications

Previous Exercise Effects in Cisplatin-Induced Renal Lesions in Rats

Previous Exercise Effects in Cisplatin-Induced Renal Lesions in Rats

Modified Huangqi Chifeng decoction inhibits excessive autophagy to protect against Doxorubicin‑induced nephrotic syndrome in rats via the PI3K/mTOR signaling pathway

Benefits of high-intensity interval training compared to continuous training to reduce apoptotic markers in female rats with cisplatin nephrotoxicity – possible modulatory role of IL-11

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