Tomatoes (Solanum
lycopersicum)
are heavy nutrient feeding crops and require high amounts of nitrogen
to maximize fruit production. The type of nitrogen applied and timing
of fertilizer applications are important to reduce losses due to volatilization
and leaching. Previous research suggested that nitrogen stable isotopes
are a useful fingerprinting system for indicating if a crop has been
grown with synthetic or organic nitrogen applications. To study the
effects of fertilization systems on nitrogen isotopic patterns, “Better
Bush” tomatoes were grown in a 2 year greenhouse experiment
to analyze nitrogen isotopic composition, nitrogen content, and fruit
yield. Three main soil fertility treatments were evaluated, and the
results were compared to those obtained on plants grown in unfertilized
soil: conventional inorganic (synthetic Miracle Grow (MG)), organic
(bonemeal and bloodmeal (BB), BB with liquid Earth Juice (BBL), BB
with 25% vermicompost (VC), BBL with 25% VC, and 25% VC), and mixed
(MG with 25% VC). The soil fertilizers, treated and untreated soil,
immature and mature leaflets tomato fruit peels, and fruit juices
were analyzed for both nitrogen isotope ratios and nitrogen concentrations.
Plant δ15Nair decreased in the order organic
treatment-no fertilizer-mixed treatment-conventional treatment. The
average δ15Nair values in leaves, fruit
peels, and juice from plants grown with organic treatments ranged
from 4.5 to 11.9, 5.4 to 10.1, and 6.1 to 11.1‰, respectively,
whereas in the case of the inorganic treatment, the average δ15Nair values varied between −3.0 and 0.4,
−1.1 and 0.4, and −0.9 and 1.9‰, respectively.
Plant nitrogen concentrations in tomato decreased in the following
order (from highest to lowest): inorganic soil fertility treatment,
mixed treatments, and organic and control (no fertilizer) treatment.
The average weight %N values in leaves and fruit peels from plants
grown with organic treatments ranged from 1.3 to 4.2 and 1.1 to 2.3%,
respectively, whereas in the case of the inorganic treatment, the
average weight %N values varied between 3.7 and 5 and 1.3 and 2.8%,
respectively. Plants grown under organic treatments have higher δ15Nair, lower weight %N, and are enriched in 15N compared with the original soil than plants grown with
inorganic fertilizer, suggesting that the synthetic nitrogen sources
are more readily available for plant uptake than the organic ones.
The addition of vermicompost increases both δ15Nair and weight %N in plants. Tomato fruit yields did not differ
between cluster 1 and cluster 2 harvest, however, total tomato fruit
yields differed indicating that synthetically fertilized plants produced
the highest total yields (g) (P ≤ 0.05). However,
all treatments with VC soil applications indicated an increase in
the amount of plant nitrogen, fruit yield, soil cation exchange capacity,
soil organic matter content, and released soil nitrogen. Nitrogen
isotope ratios of tomatoes can be used to distinguish among various
soil fertility treatments, therefore fingerprinting the organ...