Effects of preservatives and acidification on the stable isotope ratios (&lt;sup&gt;15&lt;/sup&gt;N:&lt;sup&gt;14&lt;/sup&gt;N, &lt;sup&gt;13&lt;/sup&gt;C:&lt;sup&gt;12&lt;/sup&gt;C) of two species of marine animals

Bosley, Keith L.; Wainright, Sam C.

doi:10.1139/cjfas-56-11-2181

Cited by 63 publications

(105 citation statements)

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“…Where preservation is required, freezing is often the preferred method as it typically does not aVect isotopic values. Evidence for this approach can be gathered from studies of vertebrate (Wsh), invertebrate (cephalopod, anemone and gastropod species) and algal (kelp and fucoids) species, which demonstrated isotopic values were not aVected by freezing (Bosley and Wainwright 1999;Kaehler and Pakhomov 2001;Carabel et al 2009). Quite unexpectedly, our data suggested this was not the case for gelatinous zooplankton, as freezing enriched 15 N values in A. aurita by »2‰, but did not aVect 13 C values compared with unpreserved samples.…”

Section: Discussionmentioning

confidence: 99%

“…Feuchtmayr and Grey 2003;Carabel et al 2009;Syväranta et al 2011). Although these shifts appear less pronounced than they are for 13 C (Bosley and Wainwright 1999;Kaehler and Pakhomov 2001), they are important to consider as 15 N can elucidate the trophic relationships or identify the ecological niche occupied by an organism (consumer) within a food web Epstein 1978, 1981;Post 2002;Wallace et al 2009). Ontogenetic trophic shifts have been observed in a wide range of marine mammals and Wsh (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…ctenophores) are diYcult to quantify and identify owing to fast deterioration in the oral arms and gut cavity (Båmstedt and Martinussen 2000;Ishii and Tanaka 2001;Pitt et al 2009); (2) once out of the water, gelatinous samples rapidly lose physical integrity; (3) sampling at remote sites or on research cruises of extended duration can limit access to suitable processing/analytical equipment and jellyWsh collected in the Weld are typically preserved by freezing or storage in ethanol (EtOH) (Hobson et al 1997). Preservation methods employed prior to stable isotope analysis (SIA) have been shown to alter isotopic composition in a range of marine taxa ranging from algae (Kaehler and Pakhomov 2001;Carabel et al 2009) through to invertebrates (Bosley and Wainwright 1999;Kaehler and Pakhomov 2001;Carabel et al 2009) and higher vertebrates (Bosley and Wainwright 1999;Kaehler and Pakhomov 2001). Mateo et al (2008) additionally highlighted pre-analytical biases at the class level with the greatest eVects exhibited by Maxillopoda, Gastropoda and Polychaeta.…”

Section: Introductionmentioning

confidence: 99%

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Preservation methods alter stable isotope values in gelatinous zooplankton: implications for interpreting trophic ecology

et al. 2011

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JellyWsh are increasingly topical within studies of marine food webs. Stable isotope analysis represents a valuable technique to unravel the complex trophic role of these long-overlooked species. In other taxa, sample preservation has been shown to alter the isotopic values of species under consideration, potentially leading to misinterpretation of trophic ecology. To identify potential preservation eVects in jellyWsh, we collected Aurelia aurita from Strangford Lough (54 o 22Ј44.73ЉN, 5 o 32Ј53.44ЉW) during May 2009 and processed them using three diVerent methods prior to isotopic analysis (unpreserved, frozen and preserved in ethanol). A distinct preservation eVect was found on 15 N values: furthermore, preservation also inXuenced the positive allometric relationship between individual size and 15 N values. Conversely, 13 C values remained consistent between the three preservation methods, conXicting with previous Wndings for other invertebrate, Wsh and mammalian species. These Wndings have implications for incorporation of jellyWsh into marine food webs and remote sampling regimes where preservation of samples is unavoidable.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Preservation methods alter stable isotope values in gelatinous zooplankton: implications for interpreting trophic ecology

et al. 2011

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“…Anticlotting factors were not employed to avoid the alteration of the isotopic signal (Bosley and Wainright, 1999).…”

Section: Samplingmentioning

confidence: 99%

Change in the foraging strategy of female South American sea lions (Carnivora: Pinnipedia) after parturition

Drago¹,

Cardona²,

Crespo³

et al. 2010

Sci. Mar.

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SUMMARY: This study tests the hypothesis that female South American sea lions shift from off-shore, pelagic prey to coastal, benthic prey after parturition in order to reduce the foraging trip duration and hence the time pups remain unattended on the beach during early lactation. The δ 13 C and δ 15 N values of the serum and blood cells of 26 South American sea lion suckling pups from northern Patagonia were used to track the dietary changes of their mothers from late pregnancy to early lactation, after correction for differential isotopic fractionation between tissues. Primary producers and potential prey species were also analysed to establish a baseline for interpreting the stable isotope concentration of serum and blood cells. Isotopic ratios revealed a generalized increase in the consumption of coastal-benthic prey after parturition. Such a generalized postpartum shift will allow females to spend more time on land and look after their pups. The effects of this foraging strategy on the nutritional quality of the female's diet are discussed.Keywords: South American sea lion, diet, feeding strategy, suckling pup, lactating females, Otaria flavescens, stable isotopes, Bayesian mixing models. RESUMEN: Cambio en la estrategia de alimentación de las hembras del león marino sudamericano (Carnivora: Pinnipedia) tras el parto. -El presente estudio pretende comprobar si las hembras del león marino sudamericano adoptan una dieta más bentónica y costera tras el parto, con el fin de reducir la duración de los viajes de alimentación y reducir así el tiempo durante el cual las crías permanecen desatendidas en la playa. Para ello, se determinaron las concentraciones de isótopos estables de carbono y nitrógeno en el suero y las células sanguíneas de 26 crías lactantes de león marino sudamericano del norte de la Patagonia, con el fin de reconstruir la dieta de la madre durante la parte final de la gestación y el inicio de la lactancia, tras la correspondiente corrección del fraccionamiento isotópico entre tejidos. También se analizaron muestras de productores primarios y de presas potenciales para ayudar en la interpretación de los resultados. Las concentraciones de isótopos estables antes y después del parto confirmaron un incremento en el consumo de presas bentónicas costeras tras el parto. Este cambio generalizado de dieta tras el parto permitiría a las hembras incrementar el tiempo que pasan junto a las crías. Se discuten los efectos que esta estrategia puede tener sobre la calidad de la dieta de las hembras.Palabras clave: león marino sudamericano, dieta, estrategias tróficas, lactancia, Otaria flavescens, isótopos estables, modelos de mezcla bayesianos.

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“…Individuals were then fasted for 24 h to ensure the evacuation and stomach emptying, in order to include only assimilated substances in the analysis. Snail feces were siphoned out and individuals kept in a freezer at -20 °C until their processing, and the shells were mechanically removed and discarded for the isotopic and stoichiometric analyses of C and N (Bosley and Wainright, 1999).…”

Section: Stable Isotopes Analysis (Sia)mentioning

confidence: 99%

Dieta de Pomacea canaliculata (Gastropoda: Ampullariidae) en su hábitat natural basada en análisis de contenidos digestivos e isótopos estables

et al. 2016

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Pomacea canaliculata is native to Rio de la Plata basin, and it is invasive in most of South and Southeast Asia after accidental introduction during unsuccessful attempts to establish commercial aquaculture of this species, and being present, the number one pest of rice crops in the region. Investigations in its native habitat are still needed because fundamental issues of its trophic ecology remain unknown. The aim of this research was to study the diet of P. canaliculata in its natural habitat through gut content techniques and stable isotopes of 13C and 15N. Biological samples were taken from November 2009 to December 2010 in Carnaval stream, a characteristic Pampean stream. Samples for stable isotopes of 13C and 15N using of a mixing model were collected in November 2011. The trophic strategy diagram evinced a generalist diet with high consumption of detritus followed by the vascular plants and algae. The mixing model results indicated that the relative contribution to the diet was similar for all basal resources: fine and coarse particulate organic matter, epipelon and aquatic macrophytes (approximately 40 %). These results evidenced a strong dietary plasticity for the species.

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Effects of preservatives and acidification on the stable isotope ratios (<sup>15</sup>N:<sup>14</sup>N, <sup>13</sup>C:<sup>12</sup>C) of two species of marine animals

Cited by 63 publications

References 0 publications

Preservation methods alter stable isotope values in gelatinous zooplankton: implications for interpreting trophic ecology

Preservation methods alter stable isotope values in gelatinous zooplankton: implications for interpreting trophic ecology

Change in the foraging strategy of female South American sea lions (Carnivora: Pinnipedia) after parturition

Dieta de Pomacea canaliculata (Gastropoda: Ampullariidae) en su hábitat natural basada en análisis de contenidos digestivos e isótopos estables

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