Effects of preparative procedures on the sedimentation behaviour of 40-S ribosomal subunits

Manchester, Keith

doi:10.1016/0167-4781(84)90094-0

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1984

1991

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“…Only a very slight effect of tRNA was observed without the factor. In a more recent paper, the addition of ribosomal salt-wash, but not of tRNA, was also reported to increase monomers (20). The differences with our results, which clearly show that tRNA is able to convert pratically all the dimers into monomers without the addition of any factor, must be related to the different experimental conditions used in these studies.…”

Section: Discussioncontrasting

confidence: 57%

tRNA binding modifies the properties of the small ribosomal subunit of rat liver

1984

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The binding of a specific tRNA (acylated or not) to the 40S subunits in the presence of the proper codon was shown to produce two striking effects on the subunits. First, the subunits were no longer able to dimerize at low ionic strength. Second, they became fully resistant to 1.25 M LiCl treatment: bound tRNA prevented subunit inactivation as measured by polyphenylalanine synthesis; it also prevented large sedimentation changes of subunits and ribosomal protein release induced by LiCl. The

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Section: Discussioncontrasting

confidence: 57%