Effects of prenatal capsaicin treatment on fetal spontaneous activity, opiate receptor binding, and acid phosphatase in the spinal cord

Kirby, Margaret L.; Gale, Thomas F.; Mattio, Thomas G.

doi:10.1016/0014-4886(82)90210-2

Cited by 18 publications

(9 citation statements)

References 15 publications

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“…Atkinson & Chaggar (1983) clearly demonstrated that in 7-day old mice treated with capsaicin at gestional day 15, substance P was depleted from various parts of primary neurones, however, they did not know whether the observed depletion was due to nerve destruction with permanent loss of their neuropeptide content or not. Kirby et a/. (1982) demonstrated that opiate receptor binding was affected by prenatal capsaicin treatment at gestional day 16 or 17, but was back to basal values in 24-day old rats.…”

Section: Discussionmentioning

confidence: 95%

“…While numerous works have documented these effects after administration of the neurotoxin to neonatal or adult animals, very few have examined the effects of capsaicin administered during gestation (Kirby et al 1982;Atkinson & Chaggar 1983;Pellicer et al 1996), even though it has been indirectly demonstrated that capsaicin crosses the placenta and exerts a toxic effect on the peripheral nerves of foetuses, provoking extensive depletion of substance P immunoreactive fibers from the dorsal horn of the spinal cord (Atkinson & Chaggar 1983).…”

mentioning

confidence: 99%

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Rat Offspring Treated Prenatally with Capsaicin Do Not Show Some of the Irreversible Effects Induced by Neonatal Treatment with Neurotoxin

Perfumi

Sparapassi

1999

Pharmacology & Toxicology

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Abstrrrc!: The present study evaluates whether some of the irreversible effects induced by neonatally administered capsaicin are present in offspring prenatally treated with the neurotoxin as well, and investigated its foetal toxicity. Capsaicin was administered subcutaneously at doses of 50, 100 and 200 mg/kg in five injections every other day between gestational days 7-15. the period of major organogenesis, or in a single subcutaneous injection of 50 mgikg only at day 15 of gestation. In one-month old rats prenatally capsaicin-treated the response to noxious stimulation (hot plate and wiping tests) and the urinary excretion in response to oral water load were evaluated. Parallel experiments were conducted in one-month old rats treated with capsaicin (SO mgikg) on the 2nd day of life. Prenatal capsaicin induced no evident treatment-related signs of toxicity in dams and offspring, nor did it influence the body growth of the pups or induce cutaneous lesions. Unlike neonatal treatment, prenatal administration of neurotoxin did not raise the threshold to thermal and chemical pain, and did not modify diuresis induced by oral load. Since researchers have proposed the existence of more than one population of capsaicin-sensitive afferent neurones which differ in their age-dependent sensitivity to capsaicin, we hypothesized that failure of prenatal treatment might be due either to reduced foetal availability, not capable of selectively destroying capsaicin-sensitive neurones, or to incomplete rearrangement and maturation of developing primary sensory neurons, in fact, the existence is well known of more than one population of capsaicin-sensitive afferent neurons which differ in their age-dependent sensitivity to capsaicin. Future studies are needed to elucidate the effects of capsaicin at cellular and molecular levels.Capsaicin is a potent neurotoxin which acts on a specific population of sensory neurones which contain neuropeptides

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Section: Discussionmentioning

confidence: 95%

mentioning

confidence: 99%

Rat Offspring Treated Prenatally with Capsaicin Do Not Show Some of the Irreversible Effects Induced by Neonatal Treatment with Neurotoxin

Perfumi

Sparapassi

1999

Pharmacology & Toxicology

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“…The potential teratogenicity of capsaicin has been studied by Kirby et al (302). No gross malformations were observed in offspring of rats given injec tions of capsaicin at various stages of gestation.…”

Section: Mutagenicity and Teratogenicitymentioning

confidence: 99%

“…It is used in electronic devices, photocopying, pigments, dyes, and antidandruff shampoo, as pesticides and veterinary medicine, and in the manufacture of glass. The toxicology (298,299), biochemistry (300,301), and environmental significance (297,302,303) of selenium have been reviewed and were the focus of several symposia (304)(305)(306) in the mid-1980s. Selenium is widely distributed in the environment and is present in various foodstuffs (particularly animal kidney and liver and some seafoods) (see 297).…”

Section: Other Metals and Metalloidsmentioning

confidence: 99%

Structure–Activity Relationships

WOO¹,

LAI²,

ARCOS³

et al. 1988

Natural, Metal, Fiber, and Macromolecular Carcinogens

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“…Antenatal or perinatal exposure to opioid agonists or antagonists alters the apparent number of opioid receptors in the brain with concomitant changes in antinociceptive responses (Handelmann and Quirion, 1983;Tempel et al, 1988). Similarly, stress, pain, and a variety of other drugs and toxins have been shown to alter receptor number (Torda, 1978; Kirby et al, 1982;Watanabe et al, 1983;Moon, 1984). However, the intracellular factors that regulate opioid receptor synthesis, insertion, and degradation are unknown.…”

mentioning

confidence: 99%

Characterization of Opioid Receptors in Cultured Neurons

Vaysse¹,

Zukin²,

Fields

et al. 1990

Journal of Neurochemistry

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The appearance of mu-, delta-, and kappa-opioid receptors was examined in primary cultures of embryonic rat brain. Membranes prepared from striatal, hippocampal, and hypothalamic neurons grown in dissociated cell culture each exhibited high-affinity opioid binding sites as determined by equilibrium binding of the universal opioid ligand (-)-[3H]bremazocine. The highest density of binding sites (per mg of protein) was found in membranes prepared from cultured striatal neurons (Bmax = 210 +/- 40 fmol/mg protein); this density is approximately two-thirds that of adult striatal membranes. By contrast, membranes of cultured cerebellar neurons and cultured astrocytes were devoid of opioid binding sites. The opioid receptor types expressed in cultured striatal neurons were characterized by equilibrium binding of highly selective radioligands. Scatchard analysis of binding of the mu-specific ligand [3H]D-Ala2,N-Me-Phe4,Gly-ol5-enkephalin to embryonic striatal cell membranes revealed an apparent single class of sites with an affinity (KD) of 0.4 +/- 0.1 nM and a density (Bmax) of 160 +/- 20 fmol/mg of protein. Specific binding of (-)-[3H]bremazocine under conditions in which mu- and delta-receptor binding was suppressed (kappa-receptor labeling conditions) occurred to an apparent single class of sites (KD = 2 +/- 1 nM; Bmax = 40 +/- 15 fmol/mg of protein). There was no detectable binding of the selective delta-ligand [3H]D-Pen2,D-Pen5-enkephalin. Thus, cultured striatal neurons expressed mu- and kappa-receptor sites at densities comparable to those found in vivo for embryonic rat brain, but not delta-receptors.

show abstract

Effects of prenatal capsaicin treatment on fetal spontaneous activity, opiate receptor binding, and acid phosphatase in the spinal cord

Cited by 18 publications

References 15 publications

Rat Offspring Treated Prenatally with Capsaicin Do Not Show Some of the Irreversible Effects Induced by Neonatal Treatment with Neurotoxin

Rat Offspring Treated Prenatally with Capsaicin Do Not Show Some of the Irreversible Effects Induced by Neonatal Treatment with Neurotoxin

Structure–Activity Relationships

Characterization of Opioid Receptors in Cultured Neurons

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