Effects of Pregnancy-Specific Glycoproteins on Trophoblast Motility in Three-Dimensional Gelatin Hydrogels

Zambuto, Samantha G.; Rattila, Shemona; Dveksler, Gabriela S.; Harley, Brendan A.C.

doi:10.1007/s12195-021-00715-7

Cited by 8 publications

(6 citation statements)

References 55 publications

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“…Conditioned media from days 3 and 6 were pooled prior to adding to spheroid cultures. Spheroid motility assays were performed as described previously by our group ( 47, 48, 54 ). For these studies, we used Swan 71 cells derived from a 7-week first trimester placenta; however, no additional donor information was provided ( 55 ).…”

Section: Methodsmentioning

confidence: 99%

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Endometrial decidualization status modulates endometrial perivascular complexity and trophoblast outgrowth in gelatin hydrogels

Zambuto

Theriault

Jain

et al. 2022

Preprint

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The endometrium undergoes rapid cycles of vascular growth, remodeling, and breakdown during the menstrual cycle and pregnancy. Decidualization is an endometrial differentiation process driven by steroidal sex hormones that is critical for blastocyst-uterine interfacing and blastocyst implantation. Certain pregnancy disorders may be linked to decidualization processes. However, much remains unknown regarding the role of decidualization and reciprocal trophoblast-endometrial interactions on endometrial angiogenesis and trophoblast invasion. Here, we report an artificial endometrial perivascular niche embedded in gelatin methacrylol hydrogels that displays morphological and functional patterns of decidualization. We show vessel complexity and soluble factor secretion are sensitive to decidualization and affect trophoblast motility. Importantly, we demonstrate the engineered perivascular niche can be combined with epithelial cultures to form a stratified endometrial model. This artificial perivascular niche provides a well-characterized platform to investigate dynamic changes in angiogenesis in response to pathological and physiological endometrial states.

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Section: Methodsmentioning

confidence: 99%

“…GelMA was synthesized, dialyzed, lyophilized, and was found to have a degree of functionalization of 57%, determined via 1 H-NMR (46)(47)(48). Prior to cell culture experiments, lyophilized GelMA was sterilized for 30 minutes under UV light.…”

Section: Hydrogelsmentioning

confidence: 99%

Endometrial decidualization status modulates endometrial perivascular complexity and trophoblast outgrowth in gelatin hydrogels

Zambuto

Theriault

Jain

et al. 2022

Preprint

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“…[22] A previously characterized batch of GelMA with a degree of functionalization of 57%, determined via 1 H-NMR, was utilized. [33,50,51] First, lyophilized GelMA was sterilized for 30 min under UV light. Then, cell-laden (2:1 HEMEC:HESC; 500 000 HEMEC per mL) hydrogel pre-polymer solution (5 wt% GelMA and 0.1% w/v lithium acylphosphinate in phosphate buffered saline) was prepared, the solution added to custom Teflon molds (5 mm diameter, 1 mm height), and the hydrogels were polymerized with UV (30 s; 𝜆 = 365 nm, 7.14 mW cm −2 ; AccuCure Spot System ULM-3-365).…”

Section: Methodsmentioning

confidence: 99%

Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells

Zambuto,

Jain,

Theriault

et al. 2024

Adv Healthcare Materials

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Chirality is an intrinsic cellular property that describes cell polarization biases along the left‐right axis, apicobasal axis, or front‐rear axes. Cell chirality plays a significant role in the arrangement of organs in the body as well as the orientation of organelles, cytoskeletons, and cells. Vascular networks within the endometrium, the mucosal inner lining of the uterus, commonly display spiral architectures that rapidly form across the menstrual cycle. Herein, we systematically examine the role of endometrial‐relevant extracellular matrix stiffness, composition, and soluble signals on endometrial endothelial cell chirality using a high‐throughput microarray. Endometrial endothelial cells display marked patterns of chirality as individual cells and as cohorts in response to substrate stiffness and environmental cues. Vascular networks formed from endometrial endothelial cells also display shifts in chirality as a function of exogenous hormones. Changes in cellular‐scale chirality correlate with changes in vascular network parameters, suggesting a critical role for cellular chirality in directing endometrial vessel network organization.This article is protected by copyright. All rights reserved

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“…Endometrial perivascular niche cultures were fabricated as described previously by co-culturing HEMEC and HESC in methacrylamide-functionalized gelatin (GelMA) hydrogels 20 . We utilized a previously characterized batch of GelMA with a degree of functionalization of 57%, determined via 1 H-NMR 30, 44, 45 . First, we sterilized lyophilized GelMA for 30 minutes under UV light.…”

Section: Methodsmentioning

confidence: 99%

Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells

Zambuto,

Jain,

Theriault

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Chirality is an intrinsic cellular property that describes cell polarization biases along the left-right axis, apicobasal axis, or front-rear axes. Cell chirality plays a significant role in the arrangement of organs in the body as well as the orientation of organelles, cytoskeletons, and cells. Vascular networks within the endometrium, the mucosal inner lining of the uterus, commonly display spiral architectures that rapidly form across the menstrual cycle. Herein, we systematically examine the role of endometrial-relevant extracellular matrix stiffness, composition, and soluble signals on endometrial endothelial cell chirality using a high-throughput microarray. Endometrial endothelial cells display marked patterns of chirality as individual cells and as cohorts in response to substrate stiffness and environmental cues. Vascular networks formed from endometrial endothelial cells also display shifts in chirality as a function of exogenous hormones. Changes in cellular-scale chirality correlate with changes in vascular network parameters, suggesting a critical role for cellular chirality in directing endometrial vessel network organization.

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Effects of Pregnancy-Specific Glycoproteins on Trophoblast Motility in Three-Dimensional Gelatin Hydrogels

Cited by 8 publications

References 55 publications

Endometrial decidualization status modulates endometrial perivascular complexity and trophoblast outgrowth in gelatin hydrogels

Endometrial decidualization status modulates endometrial perivascular complexity and trophoblast outgrowth in gelatin hydrogels

Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells

Cell Chirality of Micropatterned Endometrial Microvascular Endothelial Cells

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