Effects of potassium chloride on ethanol production by an osmotolerant mutant of Zymomonas mobilis

Park, S. C.; Baratti, J.

doi:10.1007/bf00242952

Cited by 13 publications

(10 citation statements)

References 24 publications

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“…Swings and De Ley tested various concentrations of glucose and found that all strains grew with 20% (1.11 M) glucose (within 34 h), 88% of the strains grew with 30% (1.66 M) glucose (within 2 to 5 days), and with 40% (2.22 M) glucose, 54% of the strains developed after lag phases of 4 to 20 days (28). In contrast, Z. mobilis is known to be rather sensitive to salt, as no strains grew at 2% NaCl (0.34 M) or 2% KCl in liquid media (22,28). Therefore, sugar tolerance need not be linked to osmotolerance or salt tolerance in general.…”

mentioning

confidence: 99%

Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection

et al. 1994

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The gram-negative ethanologenic bacterium Zymomonas mobilis is able to grow in media containing high concentrations of glucose or other sugars. A novel compatible solute for bacteria, sorbitol, which enhances growth of Z. mobilis at glucose concentrations exceeding 0.83 M (15%), is described. Added sorbitol was accumulated intracellularly up to 1 M to counteract high external glucose concentrations (up to 1.66 M or 30o). Accumulation of sorbitol was triggered by a glucose upshift (e.g., from 0.33 to 1.27 M or 6 to 23%) and was prevented by the uncoupler CCCP (carbonyl cyanide m-chlorophenylhydrazone; 100 tIM). The sorbitol transport system followed Michaelis-Menten kinetics, with an apparent Km of 34 mM and a Vm.. of 11.2 nmol -min-' mg-' (dry mass). Sorbitol was produced by the cells themselves and was accumulated when growing on sucrose (1 M or 36%) by the action of the periplasmic enzyme glucose-fructose oxidoreductase, which converts glucose and fructose to gluconolactone and sorbitol. Thus, Z. mobilis can form and accumulate the compatible solute sorbitol from a natural carbon source, sucrose, in order to overcome osmotic stress in high-sugar media. No other major compatible solute (betaine, proline, glutamate, or trehalose) was detected.The gram-negative, strictly fermentative and ethanologenic bacterium Zymomonas mobilis tolerates high concentrations of both ethanol (up to 13% [24]) and sugar (16,28) in the growth medium. An early report stated that Z. mobilis, (then called Termobacterium mobile), which had been isolated from pulque

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mentioning

confidence: 99%

Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection

et al. 1994

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“…However, these values did not interfere in ethanol production because conversion of sugar consumption into biomass was 3. Several authors have reported levan production with reduction on ethanol production (10,17). Levan production in Media II and I was 1.72 g/L and 1.56 g/L, respectively.…”

Section: Resultsmentioning

confidence: 98%

“…On the other hand, the use of high initial sugar concentration was described as a cause of incomplete utilisation of sugar (9,23). Nevertheless, there has been only few publications on sugar cane juice fermentation by Z. mobilis (5,8,17,23).…”

Section: Introductionmentioning

confidence: 99%

Effect of the presence of initial ethanol on ethanol production in sugar cane juice fermented by Zymomonas mobilis

Tano¹,

Buzato²

2003

Braz. J. Microbiol.

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Ethanol production in sugar cane juice in high initial sugar concentration, fermented by Z. mobilis in the presence and absence of ethanol, was evaluated. Ethanol production was low in both media. The presence of initial ethanol in the sugar cane juice reduced ethanol production by 48.8%, biomass production by 25.0% and the total sugar consumption by 28.3%. The presence of initial ethanol in the medium did not affect significantly levan production and biomass yield coefficient (g biomass/g sugar consumed).

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“…Apesar da alta disponibilidade de açúcar (ARTi) no meio, observou-se um baixo consumo de açúcar e elevada quantidade de ARTr (residual), que é dada pela diferença do ARTi -ARTc (consumido), no final das fermentações. Esses consumos insatisfatórios de açucar podem ser resultantes da osmolaridade do meio e, também, da presença de inibidores no caldo de cana (JOACHIMSTHAL et al,1998;PARK;BARATTI, 1993;TANO et al, 2000) Paralelamente, as concentrações de ARr (residual) encontradas no final das fermentações foram 60,75; 49,38 e 50,33 g/L, respectivamente, demonstrando uma utilização incompleta de monossacarídeos, que se acumularam no caldo de fermentação (Tabela 1). Rogers et al (1982) observaram que aproximadamente 10% da sacarose (ARTc) foram convertidos para levana por Z. mobilis ATCC 31821.…”

Section: Resultsunclassified

Influência do PH Inicial do Caldo de Cana-de-Açúcar na Produção de Levana por Zymomonas Mobilis ATCC 31821

Tano

Buzato

2002

Semina: Tech. Ex.

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Effects of potassium chloride on ethanol production by an osmotolerant mutant of Zymomonas mobilis

Cited by 13 publications

References 24 publications

Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection

Sorbitol promotes growth of Zymomonas mobilis in environments with high concentrations of sugar: evidence for a physiological function of glucose-fructose oxidoreductase in osmoprotection

Effect of the presence of initial ethanol on ethanol production in sugar cane juice fermented by Zymomonas mobilis

Influência do PH Inicial do Caldo de Cana-de-Açúcar na Produção de Levana por Zymomonas Mobilis ATCC 31821

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