Effects of postovulatory oviduct changes and female stress on aging of mouse oocytes

Zhao

et al. 2022

Front. Vet. Sci.

The oviduct consists of three parts: the infundibulum (In), ampulla (Am), and isthmus (Is). These have the same histological structure, but different physiological functions. In this study, transcriptomics was used to analyze mRNA in these three parts of yak oviduct. The results showed that there were 325 up-regulated genes and 282 down-regulated genes in the infundibulum and ampulla. Moreover, there were 234 up-regulated genes and 776 down-regulated genes in the isthmus and ampulla, as well as 873 up-regulated genes and 297 down-regulated genes in the infundibulum and isthmus. The expression of C3 in the infundibulum was significantly higher than that in the ampulla and isthmus. The expression of FAU in the isthmus was significantly lower than that in the ampulla and infundibulum, and the expression of EEF1A1 in the ampulla was significantly higher than that in the ampulla and infundibulum. When the infundibulum was compared with the ampulla and isthmus, it was found that the up-regulated genes were enriched in the lysosome, phagosome, staphylococcus aureus infection, and leishmaniasis pathway. When the isthmus was compared with the ampulla and infundibulum, the up-regulated genes were present in the apoptosis pathway, oxidative phosphorylation, and viral myocarditis pathway. When the isthmus was compared with the infundibulum and ampulla, the down-regulated pathways were protein processing in the endoplasmic reticulum and the endocytosis. The Epstein–Barr virus infection pathway was up-regulated according to a comparison of the isthmus and infundibulum and was down-regulated based on a comparison of the isthmus and ampulla. Transcriptional misregulation in the Middle East pathway was up-regulated based on a comparison of the isthmus and ampulla and was down-regulated based on a comparison of the isthmus and infundibulum. ERBB2, JUP, CTNND1, and KRT7 were defined as the hub genes of the yak oviduct. The results of this study provide sufficient omics data for yak fertilization, which is also of great significance to altitude medicine.

Section: Discussionmentioning

confidence: 99%

Identification of key genes and biological pathways in different parts of yak oviduct based on transcriptome analysis

Zhao

et al. 2022

Front. Vet. Sci.

“…What's more, it has been thought that apoptosis is a significant kind of cell death in oocyte loss. 25,26 Necroptosis is activated by pro-inflammatory signal transduction as well as ischemic injury and viral infection displaying Plasma membrane rupture and cell enlargement. The activation and assembly of necrosome complexes containing receptor-interacting serine/threonine protein kinase 1 (RIPK1) and RIPK3 will lead to phosphorylation and oligomerization of MLKL, which will then lead to lipid peroxidation, cation influx, and ultimately cell death.…”

Section: Ferroptosis and Its Activation Mechanismmentioning

confidence: 99%

Ferroptosis in female reproductive diseases: from potential pathogenesis to therapy

Chen

et al. 2023

Preprint

Ferroptosis, a cohering network integrating iron, amino acids, lipids, and redox chemicals together，is a unique regulated cell death (RCD). Iron overload, excessive reactive oxygen species (ROS), and lipid peroxidation all contribute to the onset of ferroptosis. In recent years, a growing body of evidence suggests that ferroptosis is associated with some female reproductive diseases. The purpose of our review is to give a brief description of ferroptosis activation mechanism and relationship to female reproductive diseases including infertility, pregnancy associated diseases and ovarian cancer.

“…However, the mechanisms underlying increased STAS levels in aging oocytes are largely unclear. Kong et al [11] and Chen et al [12] have reported that cytoplasmic calcium is correlated with STAS in aging mouse oocytes. Cui et al [13] observed that cytoplasmic Ca 2+ levels were increased in rat oocytes undergoing spontaneous activation during postovulatory aging.…”

Section: Introductionmentioning

confidence: 99%

Role of calcium-sensing receptor in regulating activation susceptibility of postovulatory aging mouse oocytes

Yang

Journal of Reproduction and Development

et al. 2023

Self Cite

The mechanisms underlying postovulatory oocyte aging (POA) remain largely unknown. The expression of the calcium-sensing receptor (CaSR) in mouse oocytes and its role in POA need to be explored. Our objective was to observe CaSR expression and its role in the susceptibility to activating stimuli (STAS) in POA mouse oocytes. The results showed that, although none of the newly ovulated oocytes were activated, 40% and 94% of the oocytes recovered 19 and 25 h after human chorionic gonadotropin (hCG) injection were activated, respectively, after ethanol treatment. The level of the CaSR functional dimer protein in oocytes increased significantly from 13 to 25 h post hCG. Thus, the CaSR functional dimer level was positively correlated with the STAS of POA oocytes. Aging in vitro with a CaSR antagonist suppressed the elevation of STAS, and cytoplasmic calcium in oocytes recovered 19 h post hCG, whereas aging with a CaSR agonist increased STAS, and cytoplasmic calcium of oocytes recovered 13 h post hCG. Furthermore, the CaSR was more important than the Na-Ca 2+ exchanger in regulating oocyte STAS, and T-and Ltype calcium channels were inactive in aging oocytes. We conclude that the CaSR is involved in regulating STAS in POA mouse oocytes, and that it is more important than the other calcium channels tested in this connection.