Effects of posterior vitreous detachment on aqueous humour levels of VEGF and inflammatory cytokines

Takahashi, Hidenori; Nomura, Yoko; Tan, Xue; Fujino, Yujiro; Kawashima, Hidetoshi; Yanagi, Yasuo

doi:10.1136/bjophthalmol-2014-306051

Cited by 22 publications

(14 citation statements)

References 25 publications

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“…It has been shown that intraocular cytokine concentration may change after intravitreal injection if a posterior vitreous detachment occurs 27 , 28 .…”

Section: Discussionmentioning

confidence: 99%

Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema

Mastropasqua

D’Aloisio

Nicola

et al. 2018

Sci Rep

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The aim of this work was to investigate the changes in aqueous humor cytokine levels after intravitreal injection of aflibercept in diabetic macular edema (DME) and to evaluate the relationship between cytokines modifications and central macular thickness (CMT) and retinal/choroidal vascular changes using structural and functional optical coherence tomography (OCT). Aqueous concentrations of 38 cytokines were measured via multiplex bead assay. In addition, spectral domain OCT and OCT angiography with SSADA software (XR Avanti® AngioVue) were performed at baseline and after intravitreal injections. VEGF, IL-6, IL-5, IL-1β, Eotaxin, GRO, IL-12p40, IL-12p70, IL-1RA, Flt-3L and IP-10 showed a statistically significant decrease through the follow-up (p < 0.05; p < 0.001), while Fraktalkine and GM-CSF significantly increased (p < 0.05). Best corrected visual acuity significantly increased and CMT significantly decreased during follow-up (p < 0.001 and p = 0.013). Superficial capillary plexus and deep capillary plexus density significantly increased (p < 0.001 and p = 0.014). A positive relation was found between GRO, VEGF, Fraktalkine, IP-10, IL-12p70 aqueous humor levels and CMT (p < 0.05; p < 0.001). Aflibercept is a primary anti-VEGF treatment producing a decrease of DME due to the reduction of vascular permeability, nevertheless other inflammatory cytokines showed modification after aflibercept intravitreal injections probably related to edema modification or to an interaction of aflibercept with other inflammatory cytokines.

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“…It has been shown that intraocular cytokine concentration may change after intravitreal injection if a posterior vitreous detachment occurs 27 , 28 .…”

Section: Discussionmentioning

confidence: 99%

Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema

Mastropasqua

D’Aloisio

Nicola

et al. 2018

Sci Rep

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“…Posterior vitreous detachment (PVD) was examined by B-mode ultrasonography (UD-6000), as previously detailed elsewhere. 13 14 Briefly, the mobility of the posterior vitreous during ocular saccades was examined using the ‘through the lid contact’ technique. If the posterior vitreous was detached from the retinal surface and motile with eye movements, the eyes were categorised as having complete PVD (PVD group); otherwise, the eyes were categorised as the without PVD group.…”

Section: Methodsmentioning

confidence: 99%

Changes in multiple cytokine concentrations in the aqueous humour of neovascular age-related macular degeneration after 2 months of ranibizumab therapy

Sakamoto

Takahashi²,

Tan

et al. 2017

Br J Ophthalmol

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PurposeTo determine changes in multiple cytokine concentrations in the anterior chamber during the induction phase of ranibizumab treatment in patients with neovascular age-related macular degeneration (AMD).MethodsThis prospective study included 48 treatment-naïve neovascular AMD eyes of 48 patients who received three consecutive monthly injections of ranibizumab at the Japan Community Health Care Organization Tokyo Shinjuku Medical Center between November 2010 and August 2012. We collected ~0.2 mL aqueous humour before the first and third (2 months later) injections. Controls were 80 eyes with cataracts without retinal disease. The cytokines C-X-C motif chemokine ligand 1 (CXCL1), interferon-γ-induced protein 10 (IP-10), C-X-C motif chemokine ligand 12 (CXCL12), C-X-C motif chemokine ligand 13 (CXCL13), monocyte chemoattractant protein 1 (MCP-1), CCL11, C-C motif chemokine ligand 11 (CCL11), interleukin-6 (IL-6), interleukin-10 (IL-10) and matrix metalloproteinase 9 (MMP-9) were analysed using multiplex cytokine assays.ResultsMean ages of the patients with AMD and controls were 73 and 75 years, respectively, and 31 (65%) and 37 (46%) subjects were men, respectively. Polypoidal choroidal vasculopathy was found in 27 eyes (56%). Mean concentrations of cytokines in aqueous humour in patients with neovascular AMD before the first and third ranibizumab injections were as follows (in pg/mL): CXCL1, 8.4 and 3.3; IP-10, 110 and 55; CXCL12, 480 and 240; CXCL13, 9.2 and 2.6; MCP-1, 620 and 220; CCL11, 7.1 and 2.8; IL-6, 5.9 and 1.6; IL-10, 0.15 and 0.015 (all p<0.0001), and MMP-9, 0.92 and 1.5 (p=0.0216), respectively. Concentrations of all cytokines decreased significantly after two consecutive ranibizumab injections, except for MMP-9, which increased significantly.ConclusionsAfter two monthly consecutive antivascular endothelial growth factor injections, inflammatory cytokine levels in the aqueous humour of the eyes with AMD were strongly suppressed, while MMP-9 levels increased.

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“…For the measurement of axial length, A-mode ultrasonography (UD-6000; Tomey Corp., Aichi, Japan) was used. B-mode ultrasonography (UD-6000) was used To examine posterior vitreous detachment (PVD), as previously described [16][17][18][19][20][21], and the eyes were either categorised into the PVD group or the without PVD group.…”

Section: Clinical Examinationsmentioning

confidence: 99%

“…Further studies including the balance of extracellular matrix remodelling factors such as TIMP/MMP [53] are required to understand how low concentrations of MMP-9 are associated with the number of injections. CXCL12 is a chemotactic factor for lymphocytes and promotes angiogenesis [54,55]. Interestingly, bone marrow-derived cells distributed in a mouse laserinduced CNV model are CXCL12 receptor-positive [56].…”

Section: Number Of Injections and Cytokinesmentioning

confidence: 99%

Aqueous humour proteins and treatment outcomes of anti-VEGF therapy in neovascular age-related macular degeneration

et al. 2020

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We aimed to construct a better model for predicting treatment outcomes of anti-vascular endothelial growth factor therapy for neovascular age-related macular degeneration (nAMD) using the concentrations of aqueous humour proteins at baseline and during treatment. From the data of 48 treatment-naïve nAMD eyes that received intravitreal ranibizumab pro re nata for up to 12 months, we used the aqueous humour concentrations of C-X-C motif chemokine ligand 1 (CXCL1), CXCL12, CXCL13, interferon-γ-induced protein 10, monocyte chemoattractant protein 1 (MCP-1), CC motif chemokine ligand 11, interleukin 6 (IL-6), IL-10, and matrix metalloproteinase 9 (MMP-9). After stepwise regression, multivariate analysis was performed to identify which predictors were significantly associated with best-corrected visual acuity (BCVA) changes and the number of injections. The results demonstrated that besides male sex (β coefficient = −0.088, P = 0.040) and central retinal thickness (β coefficient = 0.00051 per μm, P = 0.027), MCP-1 (β coefficient = 0.44, P < 0.001) and IL-10 (β coefficient = −0.16, P = 0.033) were significantly correlated with baseline BCVA. Additionally, high MCP-1 at baseline (β coefficient = −0.20, P = 0.015) and low CXCL13 at baseline (β coefficient = 0.10, P = 0.0054) were independently associated with better BCVA change at 12 months. High MMP-9 at the first injection (β coefficient = 0.56, P = 0.01), CXCL12 at the third injection (β coefficient = 0.10, P = 0.0002), and IL-10 at the third injection (β coefficient = 1.3, P = 0.001) were predictor variables associated with the increased number of injections. In conclusion, aqueous humour protein concentrations may have predictive abilities of BCVA change over 12 months and the number of injections in pro re nata treatment of exudative nAMD.

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Effects of posterior vitreous detachment on aqueous humour levels of VEGF and inflammatory cytokines

Abstract: PVD is related to the change in the multiple intraocular inflammatory cytokines.

Cited by 22 publications

References 25 publications

Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema

Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema

Changes in multiple cytokine concentrations in the aqueous humour of neovascular age-related macular degeneration after 2 months of ranibizumab therapy

Aqueous humour proteins and treatment outcomes of anti-VEGF therapy in neovascular age-related macular degeneration

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