Additive manufacturing (AM) has emerged as a disruptive
technique
within healthcare because of its ability to provide personalized devices;
however, printed metal parts still present surface and microstructural
defects, which may compromise mechanical and biological interactions.
This has made physical and/or chemical postprocessing techniques essential
for metal AM devices, although limited fundamental knowledge is available
on how alterations in physicochemical properties influence AM biological
outcomes. For this purpose, herein, powder bed fusion Ti-6Al-4V samples
were postprocessed with three industrially relevant techniques: polishing,
passivation, and vibratory finishing. These surfaces were thoroughly
characterized in terms of roughness, chemistry, wettability, surface
free energy, and surface ζ-potential. A significant increase
in Staphylococcus epidermidis colonization
was observed on both polished and passivated samples, which was linked
to high surface free energy donor γ– values
in the acid–base, γAB component. Early osteoblast
attachment and proliferation (24 h) were not influenced by these properties,
although increased mineralization was observed for both these samples.
In contrast, osteoblast differentiation on stainless steel was driven
by a combination of roughness and chemistry. Collectively, this study
highlights that surface free energy is a key driver between AM surfaces
and cell interactions. In particular, while low acid–base components
resulted in a desired reduction in S. epidermidis colonization, this was followed by reduced mineralization. Thus,
while surface free energy can be used as a guide to AM device development,
optimization of bacterial and mammalian cell interactions should be
attained through a combination of different postprocessing techniques.