Effects of post-mortem intervals on regional brain protein profiles in rats using SELDI-TOF-MS analysis

Machaalani, Rita; Gozal, Evelyne; Berger, François; Waters, Karen A.; Demattéis, Maurice

doi:10.1016/j.neuint.2010.08.002

Cited by 10 publications

(7 citation statements)

References 25 publications

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“…Limited research has been devoted to the study of human remains and PMI estimations, despite the medicolegal importance of relevant studies. Instead researchers have used animal models to study decomposition and are starting to look for biomarkers that may provide potential candidates for PMI estimation (Poloz and O'Day 2009;Machaalani et al 2010;Swann et al 2010b;Tumram et al 2011;Viinamaki et al 2011;Swann et al 2010a;Donaldson and Lamont 2013b). However, few potential biomarkers have been identified, and only one study (Donaldson and Lamont 2013b) has examined blood biochemical markers for investigating PMI.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics of post-mortem blood: identifying potential markers of post-mortem interval

Donaldson

Lamont

2014

Metabolomics

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Death results in changes in some metabolites in body tissues due to lack of circulating oxygen, altered enzymatic reactions, cellular degradation, and cessation of anabolic production of metabolites and macromolecules. Metabolic changes may provide chemical markers to better determine the time since death (post-mortem interval), something that is challenging to establish with current observation-based methodologies. The aim of this research was to carry out a metabolic analysis of blood plasma postmortem, in order to gain a more complete understanding of the biochemical changes that occur following death. Gas chromatography was used to conduct a survey of post-mortem rat blood. Sixty six metabolites were detected post-mortem. Twenty six of these [18 amino acids, glutathione (GSH), 4-Amino-n-butyric acid (GABA), glyoxylate, oxalate, hydroxyproline, creatinine, a-ketoglutarate and succinate] had increased concentrations post-mortem. The remaining 40 metabolites had concentrations that were not dependant on time. This study demonstrates the range of metabolic changes that occur post-mortem as well as identifying potential markers for estimating post-mortem interval.

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Section: Discussionmentioning

confidence: 99%

Metabolomics of post-mortem blood: identifying potential markers of post-mortem interval

Donaldson

Lamont

2014

Metabolomics

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“…Limited research has been devoted to the taphonomy of human remains and PMI estimations, with the exception of the Anthropological Research Facility at the University of Tennessee which studies human decomposition and PMI, despite the medicolegal importance of relevant studies. Researchers are starting to identify biomarkers in mammals that may provide a fairly accurate PMI estimation (Machaalani et al, 2010;Poloz & O'Day, 2009;Swann et al, 2010a;Swann et al, 2010b;Tumram et al, 2011;Viinamaki et al, 2011) between 400-600g, and none of the rats used in this study were isogenic.…”

Section: Discussionmentioning

confidence: 99%

Estimation of post-mortem interval using biochemical markers

Donaldson

Lamont

2013

Australian Journal of Forensic Sciences

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“…2 and 3): Tissue from autopsies has proven invaluable in studies of diseases, including neurological diseases, but the postmortem interval (time from death to tissue preservation) can influence the results of the investigations. Changes are readily detected by proteomic analyses after 6 hours (Machaalani et al, 2010). If all proteins had been degraded at the same rate in all cells, then the post-mortem interval would only have affected the sensitivity.…”

Section: Sample Quality -Proteolysis and True Oligomers Versus In Vitmentioning

confidence: 99%

Strategies for immunohistochemical protein localization using antibodies: What did we learn from neurotransmitter transporters in glial cells and neurons

Danbolt

Zhou

Furness

et al. 2016

Glia

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Immunocytochemistry and Western blotting are still major methods for protein localization, but they rely on the specificity of the antibodies. Validation of antibody specificity remains challenging mostly because ideal negative controls are often unavailable. Further, immunochemical labeling patterns are also influenced by a number of other factors such as postmortem changes, fixation procedures and blocking agents as well as the general assay conditions (e.g., buffers, temperature, etc.). Western blotting similarly depends on tissue collection and sample preparation as well as the electrophoretic separation, transfer to blotting membranes and the immunochemical probing of immobilized molecules. Publication of inaccurate information on protein distribution has downstream consequences for other researchers because the interpretation of physiological and pharmacological observations depends on information on where ion channels, receptors, enzymes or transporters are located. Despite numerous reports, some of which are strongly worded, erroneous localization data are being published. Here we describe the extent of the problem and illustrate the nature of the pitfalls with examples from studies of neurotransmitter transporters. We explain the importance of supplementing immunochemical observations with other measurements (e.g., mRNA levels and distribution, protein activity, mass spectrometry, electrophysiological recordings, etc.) and why quantitative considerations are integral parts of the quality control. Further, we propose a practical strategy for researchers who plan to embark on a localization study. We also share our thoughts about guidelines for quality control. GLIA 2016;64:2045–2064

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Effects of post-mortem intervals on regional brain protein profiles in rats using SELDI-TOF-MS analysis

Cited by 10 publications

References 25 publications

Metabolomics of post-mortem blood: identifying potential markers of post-mortem interval

Metabolomics of post-mortem blood: identifying potential markers of post-mortem interval

Estimation of post-mortem interval using biochemical markers

Strategies for immunohistochemical protein localization using antibodies: What did we learn from neurotransmitter transporters in glial cells and neurons

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