Effects of positive charge density on the liposomal surface on disposition kinetics of liposomes in rats

Aoki, Hiromitsu; Tottori, Tsuneaki; Sakurai, Fuminori; Fuji, Kaoru; Miyajima, Koichiro

doi:10.1016/s0378-5173(97)00184-1

Cited by 31 publications

(25 citation statements)

References 26 publications

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“…Some investigators have shown that the in vivo behavior of cationic liposomes is mainly decided by their zeta potential or the amount of cationic lipid contained in the formulation. Aoki et al (1997) showed that when the cationic liposomes containd only 5 mol% of charge lipid with small zeta potential, the behavior of the cationic liposomes would not be different from that of neutral liposomes (Zalipsky et al 1994). Liposomes containing 10 mol% cationic lipid or bearing a zeta potential of ∼ +15 mV would remain in blood longer and accumulated less in liver (Aoki et al 1997;Abraham, Goundalkar, and Mezei 1984).…”

Section: Discussionmentioning

confidence: 99%

“…Aoki et al (1997) showed that when the cationic liposomes containd only 5 mol% of charge lipid with small zeta potential, the behavior of the cationic liposomes would not be different from that of neutral liposomes (Zalipsky et al 1994). Liposomes containing 10 mol% cationic lipid or bearing a zeta potential of ∼ +15 mV would remain in blood longer and accumulated less in liver (Aoki et al 1997;Abraham, Goundalkar, and Mezei 1984). Cationic liposomes containing ∼50% of charged lipid were reported to accumulate in the liver rapidly after intravenous injection, and the corresponding zeta potential may be 25 mV or above (Litzinger et al 1996).…”

Section: Discussionmentioning

confidence: 99%

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Characteristics and Biodistribution of Soybean Sterylglucoside and Polyethylene Glycol-Modified Cationic Liposomes and Their Complexes with Antisense Oligodeoxynucleotide

Yan

Maitani

et al. 2005

Drug Delivery

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A novel cationic liposome modified with soybean sterylglucoside (SG) and polyethylene glycol-distearoylphosphatidylethanolamine (PEG-DSPE) as a carrier of antisense oligodeoxynucleotide (ODN) for hepatitis B virus (HBV) therapy was constructed. Characteristics of the cationic liposomes modified with SG and PEG (SG/PEG-CL) and their complexes with 15-mer phosphorothioate ODN (SG/PEG-CL-ODN complex) were investigated by incorporation efficiency, morphology, electrophoresis, zeta potentials, and size analysis. Antisense activity of the liposomes and ODN complexes was determined as hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) in HepG2 2.2.15 cells by ELISA. Their tissue and intrahepatic distribution were evaluated following intravenous injection in mice. The complexes gained high incorporation efficiency and intact vesicular structure with mean size at approximately 200 nm. The SG/PEG-CL-ODN complexes enhanced the inhibition of both HBsAg and HBeAg expression in the cultured HepG2 2.2.15 cells relative to free ODN. The uptake of SG/PEG-CL and nonmodified cationic liposomes (CL) was primarily by liver, spleen, and lung. Furthermore, the concentration of SG/PEG-CL was significant higher than that of CL in hepatocytes at 0.5 hr postinjection. The biodistribution of SG/DSPE-CL-ODN complex compare with free ODN showed that liposomes enhanced the accumulation of ODN in the liver and spleen, while decreasing its blood concentration. SG/PEG-CL-mediated ODN transfer to the liver is an effective gene delivery method for cell-specific targeting, which has a potential for gene therapy of HBV infections. SG and PEG-modified cationic liposomes have proven to be an alternative carrier for hepatocyte-selective drug targeting.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characteristics and Biodistribution of Soybean Sterylglucoside and Polyethylene Glycol-Modified Cationic Liposomes and Their Complexes with Antisense Oligodeoxynucleotide

Yan

Maitani

et al. 2005

Drug Delivery

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“…Based on the pH gradient method previously described [27], octadecylamine and heparin sodium were added to optimize the preparation of lip-bFGF. Octadecylamine was added to improve the stability of liposomes [28]. The binding of bFGF and heparin is believed to favor the stabilization of bFGF through the formation of complexes resistant to inactivation by extreme conditions, such as high temperature, acidic conditions, and proteolysis [29].…”

Section: Methodsmentioning

confidence: 99%

Human Basic Fibroblast Growth Factor Inhibits Tau Phosphorylation via the PI3K/Akt-GSK3β Signaling Pathway in a 6-Hydroxydopamine-Induced Model of Parkinson's Disease

Yang¹,

Zhu²,

Huang³

et al. 2016

Neurodegener Dis

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Background: Basic fibroblast growth factor (bFGF) has been increasingly investigated due to its neuroprotection in neurodegenerative disorders. Because there are still no cures for any of these disorders, it is crucial to identify new therapeutic targets and screen potential drugs. The increased phosphorylation of tau at Ser³⁹⁶ leads to intracellular tau accumulation, which forms neurofibrillary tangles in Parkinson's disease (PD). In this study, neuroprotection by bFGF was observed, and the mechanisms related to its regulation of phosphorylated tau were investigated. Methods: bFGF-loaded liposome carriers were intranasally administered to rats. The neuroprotective effects of bFGF were assessed in a PD model induced by 6-hydroxydopamine (6-OHDA) in vivo and in vitro. The phosphorylation of tau was measured, and the PI3K/Akt-GSK3β signaling pathway was investigated. Results: Our study demonstrated that liposomes markedly assisted in the delivery of bFGF to the striatum and substantia nigra of rats and enhanced the neuroprotective effects of bFGF on dopaminergic neurons. bFGF treatment significantly ameliorated the behavioral deficits induced by 6-OHDA, rescued the loss of tyrosine hydroxylase-positive neurons and increased the number of Nissl bodies. bFGF reduced the phosphorylation of tau and GSK3β and increased the phosphorylation of PI3K/Akt. Conclusion: Liposomes markedly assisted in the delivery of bFGF to the brain and enhanced the neuroprotective effects of bFGF by inhibiting the phosphorylation of tau. bFGF down-regulated the phosphorylation of tau by increasing the phosphorylation of GSK3β via the PI3K/Akt signaling pathway. These findings provide a new vision of bFGF as a potential therapy for PD.

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“…Adsorption of liver specific opsonins probably enhances the uptake of Ps by liver macrophages, Kupffer cells and this process may play a major role in the hepatic uptake of the vesicles [216]. Interactions with the opsonins can be reduced by introduction of a slightly negative or positive charge on the surface of Ps, yielding prolonged blood circulation times [218,219]. However, it has been reported that either high negative or positive charge lead to more rapid clearance of carriers due to enhanced hepatic uptake [203,219,220].…”

Section: Circulation Kinetics and Biodistribution Of Polymersomesmentioning

confidence: 99%

“…Interactions with the opsonins can be reduced by introduction of a slightly negative or positive charge on the surface of Ps, yielding prolonged blood circulation times [218,219]. However, it has been reported that either high negative or positive charge lead to more rapid clearance of carriers due to enhanced hepatic uptake [203,219,220]. Likewise, a range of optimal sizes for specific nanocarriers has been suggested to establish long circulation times (e.g.…”

Section: Circulation Kinetics and Biodistribution Of Polymersomesmentioning

confidence: 99%

Biodegradable polymersomes for drug delivery : circulation kinetics and biodistribution, modulated drug delivery and cellular uptake

Lee¹

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Effects of positive charge density on the liposomal surface on disposition kinetics of liposomes in rats

Cited by 31 publications

References 26 publications

Characteristics and Biodistribution of Soybean Sterylglucoside and Polyethylene Glycol-Modified Cationic Liposomes and Their Complexes with Antisense Oligodeoxynucleotide

Characteristics and Biodistribution of Soybean Sterylglucoside and Polyethylene Glycol-Modified Cationic Liposomes and Their Complexes with Antisense Oligodeoxynucleotide

Human Basic Fibroblast Growth Factor Inhibits Tau Phosphorylation via the PI3K/Akt-GSK3β Signaling Pathway in a 6-Hydroxydopamine-Induced Model of Parkinson's Disease

Biodegradable polymersomes for drug delivery : circulation kinetics and biodistribution, modulated drug delivery and cellular uptake

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