Effects of Pore Size and Crosslinking Methods on the Immobilization of Myoglobin in SBA-15

Miao, Hengmin; Li, Maosheng; Sun, Xiaochun; Xia, Jikun; Li, Yanqing; Li, Jiao; Wang, Fang; Xu, Jiakun

doi:10.3389/fbioe.2021.827552

Cited by 11 publications

(6 citation statements)

References 37 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The band at ∼3346 cm −1 in the spectrum of free myoglobin was represented another characteristic protein band that indicates the presence of ‐CH 2 and ‐NH. The FT‐IR spectra results are quite similar to the FT‐IR spectra represented by Miao and Ovung [62,63] . In the spectrum of MbNFs@Zn, the peak in the range ∼2800–3600 cm −1 was indicated the existence of ‐CH 2 , ‐CH 3 and ‐NH groups.…”

Section: Resultssupporting

confidence: 81%

“…The FT-IR spectra results are quite similar to the FT-IR spectra represented by Miao and Ovung. [62,63] In the spectrum of MbNFs@Zn, the peak in the range ~2800-3600 cm À 1 was indicated the existence of -CH 2 , -CH 3 and -NH groups. The peak at ~1634 cm À 1 was attributed to -C=O stretching in the nanoflowers structure.…”

Section: Synthesis and Characterization Of Mbnfs@znmentioning

confidence: 99%

See 1 more Smart Citation

A Novel Biomaterial for Decolorization of Azo Dyes: Myoglobin‐Zn(II) Assisted Hybrid Nanoflowers

Altınkaynak

Turk

Yangin

et al. 2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

Protein‐inorganic hybrid nanoflowers are new multifunctional materials shown enhanced catalytic performance. Specially, they are used as catalyst and dye decolorizer via Fenton reaction. In this study, the Myoglobin‐Zn (II) assisted hybrid nanoflowers (MbNFs@Zn) were fabricated by using myoglobin and zinc (II) ions in different synthesis conditions. The optimum morphology was characterized SEM, TEM, EDX, XRD, and FT‐IR. The hemisphere and uniform morphology was obtained at pH 6 and 0.1 mg mL−1. The size of MbNFs@Zn are 5–6 μm. The encapsulation yield was ∼95 %. In the presence of H2O2, the peroxidase mimic activity of MbNFs@Zn was spectrophotometrically investigated in the different pH values (4–9). The highest peroxidase mimic activity was found as 3.378 EU/mg at pH 4. MbNFs@Zn was exhibited 0.28 EU/mg after eight cycles. MbNFs@Zn has lost about 92 % of its activity. The usability of MbNFs@Zn for decolorization of azo dyes such as Congo red (CR), and Evans blue (EB) was researched at different times, temperatures and concentrations. The decolorization efficiency was found maximum as 92.3 % and 88.4 % for EB and CR dyes, respectively. MbNFs@Zn has perfect properties such as enhanced catalytic performance, high decolorization efficiency, stability and reusability, and can be excellent potential materials for many industrial applications.

show abstract

Section: Resultssupporting

confidence: 81%

Section: Synthesis and Characterization Of Mbnfs@znmentioning

confidence: 99%

A Novel Biomaterial for Decolorization of Azo Dyes: Myoglobin‐Zn(II) Assisted Hybrid Nanoflowers

Altınkaynak

Turk

Yangin

et al. 2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

show abstract

“…Although the protein loading of the modified material is increased, the activity of the immobilized enzyme obtained from the modified mesoporous material is slightly reduced because the activity of the immobilized enzyme is directly related to the pore size of the mesoporous material. The addition of organic groups reduces the pore size of the carrier, which was previously conducive to the enzyme catalytic reaction ( Lü et al, 2008 ; Miao et al, 2022 ). The substrate and product can diffuse well in the pore channel during the reaction process, reducing the mass transfer resistance of the substrate and product.…”

Section: Resultsmentioning

confidence: 99%

Surface Functionalization of SBA-15 for Immobilization of Myoglobin

Miao

Wang

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Mesoporous molecular sieve SBA-15 was successfully modified with 3-aminopropyltriethoxysilane (APTES) and 3-glycidyloxypropyltrimethoxysilane (GPTMS). The functionalized SBA-15 were characterized by small-angle X-ray (SAXRD), thermogravimetric analysis (TG), N2 adsorption, and Fourier transformed infrared spectrum (FT-IR). APTES functionalized SBA-15 (named SBA-15-A) and GPTMS functionalized SBA-15 (named SBA-15-G) were used to immobilize myoglobin (Mb). The loading amounts of Mb by SBA-15-A and SBA-15-G were 511.2 and 547.8 mg/g, respectively, whereas only 359.6 mg/g was achieved by SBA-15. Mb/SBA-15-G and Mb/SBA-15-A demonstrated better reusability than SBA-15, retaining 84.6% and 82.7% of the initial activity after repeated use seven times. The Mb/SBA-15-A and Mb/SBA-15-G also exhibited improved thermal stability and storage stability.

show abstract

“…Concerning the support materials, the critical factors for cell integration include their porosity and capacity to adhere to cells through van der Waals interactions, ionic forces, hydrogen bonding, or other mechanisms. , The porous matrices have a high permeability that allows diffusion of oxygen and nutrients needed characteristics when into the material are encapsulated microorganisms for water purification, biomolecule production, and ethanol production . Other applications of these matrices are in tissue engineering, drug delivery systems, wound healing, as well as in the production of probiotic food, biosorption and bioremediation of chemicals, and antimicrobial food packaging .…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Design of a Synthetic-Living Material Composed of Chitosan, Calendula officinalis Hydroalcoholic Extract, and Yeast with Applications as a Biocatalyst

et al. 2023

View full text Add to dashboard Cite

Design and development of materials that couple synthetic and living components allow taking advantage of the complexity of biological systems within a controlled environment. However, their design and fabrication represent a challenge for material scientists since it is necessary to synthesize synthetic materials with highly specialized biocompatible and physicochemical properties. The design of synthetic-living materials (vita materials) requires materials capable of hosting cell ingrowth and maintaining cell viability for extended periods. Vita materials offer various advantages, from simplifying product purification steps to controlling cell metabolic activity and improving the resistance of biological systems to external stress factors, translating into reducing bioprocess costs and diversifying their industrial applications. Here, chitosan sponges, functionalized with Calendula officinalis hydroalcoholic extract, were synthesized using the freeze-drying method; they showed small pore sizes (7.58 μm), high porosity (97.95%), high water absorption (1695%), and thermal stability, which allows the material to withstand sterilization conditions. The sponges allowed integration of 58.34% of viable Saccharomyces cerevisiae cells, and the cell viability was conserved 12 h post-process (57.14%) under storage conditions [refrigerating temperature (4 °C) and without a nutrient supply]. In addition, the synthesized vita materials conserved their biocatalytic activity after 7 days of the integration process, which was evaluated through glucose consumption and ethanol production. The results in this paper describe the synthesis of complex vita materials and demonstrate that biochemically modified chitosan sponges can be used as a platform material to host living and metabolically active yeast with diverse applications as biocatalysts.

show abstract

Effects of Pore Size and Crosslinking Methods on the Immobilization of Myoglobin in SBA-15

Cited by 11 publications

References 37 publications

A Novel Biomaterial for Decolorization of Azo Dyes: Myoglobin‐Zn(II) Assisted Hybrid Nanoflowers

A Novel Biomaterial for Decolorization of Azo Dyes: Myoglobin‐Zn(II) Assisted Hybrid Nanoflowers

Surface Functionalization of SBA-15 for Immobilization of Myoglobin

Synthesis and Design of a Synthetic-Living Material Composed of Chitosan, Calendula officinalis Hydroalcoholic Extract, and Yeast with Applications as a Biocatalyst

Contact Info

Product

Resources

About