Effects of porcine epidemic diarrhea virus on porcine monocyte-derived dendritic cells and intestinal dendritic cells

Oral immunization with aLactobacillus casei-based anti-porcine epidemic diarrhoea virus (PEDV) vaccine expressing microfold cell-targeting peptide Co1 fused with the COE antigen of PEDV

“…Just as the finding that PEDV infection or vaccination can effectively stimulate T‐cell proliferation (Gao et al . ), oral immunization with strain pPG‐COE‐Co1/L393 can elicit a more robust lymphocyte proliferative response against PEDV COE.…”

Differential gene modulation of pattern-recognition receptor TLR and RIG-I-like and downstream mediators on intestinal mucosa of pigs infected with PEDV non S-INDEL and PEDV S-INDEL strains

“…PEDV S-INDEL was shown to be clinically relevant in neonates, but clinical disease could not be reproduced in pigs older than three weeks (Annamalai et al, 2015;Chen et al, 2016). The molecular mechanism of innate immune modulation has only been evaluated in vitro, and thus available information is scarce (Cao et al, 2015a(Cao et al, , 2015bDing et al, 2014a;Gao et al, 2015;Xu et al, 2013). However, it has been demonstrated that in vitro studies are highly dependent on virus strain and cell type, and are not valid for the evaluation of innate immune response against low-virulent strains (Kint et al, 2015b).…”

Porcine epidemic diarrhea virus does not replicate in porcine monocyte-derived dendritic cells, but activates the transcription of type I interferon and chemokine

“…Porcine alveolar macrophages have been shown to support PEDV replication (Park and Shin, 2014). One recent study suggests that PEDV only replicates in Mo-DC prior to 24 h after infection, but not at later time points, as determined by virus titration of culture supernatants (Gao et al, 2015). However, our data suggest that PEDV fails to replicate in Mo-DC as evidenced by the decrease of viral gene transcript over time and the absence of virus proteins and virus particles in the infected cells as examined by immunofluorescence staining and TEM respectively.…”

“…PEDV is mainly detected in the intestinal epithelial cells in infected animals. One recent study shows that PEDV undergoes an early replication in porcine monocyte-derived dendritic cells (Mo-DC) and activates the antigen-presenting ability of Mo-DC (Gao et al, 2015). Although viral antigen was detected in the dendritic-like cells in the laminar propria of small intestine of TGEV-infected animals, it remains controversial whether TGEV actually replicate in dendritic cells in vivo and in vitro (Riffault et al, 2001).…”