Effects of polyunsaturated fatty acids and hormones on synaptogenesis in serum-free medium cultures of mouse fetal hypothalamic cells

Tixier-Vidal, A; Picart, R; Loudes, Catherine; Bauman, Annie

doi:10.1016/0306-4522(86)90230-7

Cited by 60 publications

(26 citation statements)

References 38 publications

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“…In the elderly and patients with AD, low DHA and n-3 PUFA levels have been detected in the plasma (19) and brain (20), showing widespread loss of neuronal synapses. Dietary DHA has been suggested to improve neuronal development (21), restore and enhance cognitive functions (22 -24), and increase neuronal resistance to various types of insults, including amyloid-induced oxidative stress (25 -28). To clarify the mechanisms of the beneficial effects of DHA on AD, the roles of DHA in cognitive functions and AD pathology are reviewed here.…”

Section: Introductionmentioning

confidence: 99%

Neuroprotective and Ameliorative Actions of Polyunsaturated Fatty Acids Against Neuronal Diseases: Beneficial Effect of Docosahexaenoic Acid on Cognitive Decline in Alzheimer’s Disease

Hashimoto

Hossain

2011

J Pharmacol Sci

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Abstract. Docosahexaenoic acid (DHA, C22:6 n-3), the most abundant n-3 polyunsaturated fatty acid in the brain, is essential for brain growth and development. Recent evidence has indicated the potential health benefits of DHA for managing Alzheimer's disease (AD). For example, dietary administration of DHA considerably protects against and ameliorates the impairment of learning ability in amyloid-beta (Aβ) 1-40 -infused AD-model rats, with concurrent increases in DHA levels and decreases in the levels of lipid peroxide and reactive oxygen species in the cortico-hippocampal tissues. In addition, dietary DHA helps in eliminating the amyloid burden from the brains of ADmodel rats. In vitro studies have revealed that DHA substantially inhibits Aβ fibrillation. Furthermore, DHA reduces amyloid-induced toxicity in cell culture. These in vitro data support the hypothesis that DHA can ameliorate the cognitive deficits of AD in vivo by limiting Aβ polymerization in the brains. Therefore, it might be a useful therapeutic agent to prevent and/or delay cognitive impairment in mild cases of AD.

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Section: Introductionmentioning

confidence: 99%

Neuroprotective and Ameliorative Actions of Polyunsaturated Fatty Acids Against Neuronal Diseases: Beneficial Effect of Docosahexaenoic Acid on Cognitive Decline in Alzheimer’s Disease

Hashimoto

Hossain

2011

J Pharmacol Sci

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“…Recently, evidence has been presented that treatment with estrogen increased Na,K-ATPase activity in the cerebellum of rats (Litteria, 1987), a finding that adds a new facet to the well-established fact that estrogen (E2) can affect maturation of neuronal cells, originating morphological as well as biochemical changes, which are held responsible for the sexual differentiation of the central nervous system in mammals and birds (for revicw see Arnold and Gorski, 1984). Notable insight has been gained into these processes using tissue culture methods to study neurite growth (Reisert ct al., 1987;Toran-Allerand, 1985) and synapse formation (Nishizuka and Arai, 1982;Tixier-Vidal et al: 1986) in neurons taken from specific regions of the brain of rat and mouse embryos. The possibility exists that the different isoforms of Na,K-ATPase may be spatially compartmentalized throughout the neuron; therefore it seems relevant to compare neuritogenesis and Na,K-AI'Pase activity as affected by neuronal maturation and differentiation induced by E2.…”

Section: Introductionmentioning

confidence: 99%

Differentiation of rat hippocampal neurons induced by estrogen in vitro: Effects on neuritogenesis and Na,K‐ATPase activity

Blanco

Díaz

Carrer

et al. 1990

J of Neuroscience Research

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To gain insight into the mechanisms responsible for differentiation of hippocampal neurons growing in vitro, the effects of estrogen on neuritic development and on activity and distribution of isoforms of the Na, K-ATPase, were evaluated. Dissociated cells from hippocampi of 19-day-old rat fetuses were raised for 5 days in the presence or absence of 100 nM estradiol-17 beta (E2) in minimum essential medium supplemented either with 10% untreated fetal calf serum (MEM-10) or with 10% fetal calf serum previously adsorbed with dextran-activated charcoal (MEM-10-Cha). Cultures in MEM-10 showed larger neuritic length and increased levels of Na, K-ATPase activity than cultures in MEM-10-Cha. In cells cultured in MEM-10 medium, the addition of E2 resulted in selective enhancement of axonal length with a concomitant increase in the alpha-2 isoform of the Na, K-ATPase, whereas a decrease was found in the form most sensitive to ouabain; the total enzymatic activity remained unchanged. Conversely, in cultures raised in MEM-10-Cha, E2 did not affect Na, K-ATPase activity or neuritogenesis. These results show that two presumably independent probes of cellular differentiation of hippocampal neurons (i.e., neuritogenesis and patterns of Na, K-ATPase activity) were concurrently regulated by E2 and that such regulation depended on interaction with factor(s) present in calf serum. The well-known neuritogenic effect of E2 is hereby extended to hippocampal neurons, although for these cells it seems to be restricted to axons.

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“…The addition of oestradiol to the N2 medium improved cell attach¬ ment and the neuronahglial cell ratio (Faivre-Bauman et al 1981). The presence of T3 in the medium improved culture survival and induced neurite exten¬ sion (Puymirat, Barret, Picart et al 1983), while supplementation with polyunsaturated fatty acids re¬ stored the normal fatty acid profile of membranes (Bourre, Faivre, Dumont et al 1983) and played an important role in synaptogenesis (Tixier-Vidal, Picart, Loudes & Faivre-Bauman, 1986). More recently it has been suggested that the metabolites of arachidonic acid can act as second messengers in neurones (Piomelli, Volterra, Dale et al 1987) and may there¬ fore play an important role in the function of the hypothalamic peptidergic neurones.…”

Section: Discussionmentioning

confidence: 96%

Comparison of peptide release from fetal rat hypothalamic neurones cultured in defined media and serum-containing media

Clarke

Gillies²

1988

Journal of Endocrinology

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Primary cultures of rat hypothalamic neurones were maintained either in a serum-supplemented medium or in a serum-free chemically defined medium for up to 6 weeks. The release of the 41 amino acid-containing peptide, corticotrophin-releasing factor (CRF-41), vasopressin (AVP) and somatostatin (SRIF) were followed using immunoassays. In response to K+ (56 mmol/l) depolarization both the quantities of peptides released and the magnitude of responses were significantly greater from cultures maintained in the fully supplemented defined medium. As a consequence, release of CRF-41 and AVP could be measured directly, without requiring the concentration step necessary for cultures grown in serum. The response to K+ depolarization increased with the age of the culture, suggesting neuronal maturation. Responses to K+ depolarization were Ca2+-dependent, and the addition of corticosterone (100 nmol/l) to the defined medium caused a significant reduction in the response of neurones secreting CRF-41 and AVP, but not those secreting SRIF, to depolarization. This suggests the retention in vitro of the responsiveness of stress-associated neuropeptides to the negative feedback effects of corticosterone. Neurones producing CRF-41 and AVP responded significantly in a dose-dependent manner to acetylcholine stimulation, whereas those producing SRIF did not. As cultures matured, the CRF-41- and AVP-producing neurones became more sensitive to acetylcholine with the maximal response at 1 nmol acetylcholine/l. In conclusion, the culture of rat hypothalamic neurones is improved in terms of peptide output when the cultures are maintained in a defined medium.(ABSTRACT TRUNCATED AT 250 WORDS)

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Effects of polyunsaturated fatty acids and hormones on synaptogenesis in serum-free medium cultures of mouse fetal hypothalamic cells

Cited by 60 publications

References 38 publications

Neuroprotective and Ameliorative Actions of Polyunsaturated Fatty Acids Against Neuronal Diseases: Beneficial Effect of Docosahexaenoic Acid on Cognitive Decline in Alzheimer’s Disease

Neuroprotective and Ameliorative Actions of Polyunsaturated Fatty Acids Against Neuronal Diseases: Beneficial Effect of Docosahexaenoic Acid on Cognitive Decline in Alzheimer’s Disease

Differentiation of rat hippocampal neurons induced by estrogen in vitro: Effects on neuritogenesis and Na,K‐ATPase activity

Comparison of peptide release from fetal rat hypothalamic neurones cultured in defined media and serum-containing media

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