Effects of polystyrene microplastics acute exposure in the liver of swordtail fish (Xiphophorus helleri) revealed by LC-MS metabolomics

Zhang, Yankun; Yang, Bing-Kun; Zhang, Chunnuan; Xu, Shixiao; Sun, Ping

doi:10.1016/j.scitotenv.2022.157772

Cited by 24 publications

(9 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, in our results, PP-N/MPs significantly increased the metabolism level of amino acids. Amino acids are an important source of chemical energy in fish metabolism and are related to antioxidant capacity and immune response . Upregulation of amino acid signaling in liver tissues also indicated that the antioxidant capacity and immune response in tilapia were stimulated by exposure to PP-N/MPs.…”

Section: Resultsmentioning

confidence: 99%

Liver Metabolic Dysregulation Induced by Polypropylene Nano- and Microplastics in Nile Tilapia Using Internal Extractive Electrospray Ionization Mass Spectrometry

Yang

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

Understanding the metabolic disorders induced by nano-and microplastics in aquatic organisms at the molecular level could help us understand the potential toxicity of nano-and microplastics more thoroughly and provide a fundamental scientific basis for regulating the usage and management of plastic products. In this research, the effect of polypropylene nanoplastics (PP-NPs) and microplastics (PP-MPs) on metabolites in the tilapia liver was comprehensively investigated by internal extractive electrospray ionization mass spectrometry (iEESI-MS). A partial least-squares discriminant analysis (PLS-DA) and a one-component analysis of variance (ANOVA) were used for selecting 46 differential metabolites, including phospholipids, amino acids, peptides, carbohydrates, alkaloids, purines, pyrimidines, and nucleosides. Pathway enrichment analysis showed significant effects on glycerophospholipid metabolism, arginine and proline metabolism, and aminoacyl-tRNA biosynthesis after tilapia were exposed to PP-N/MPs. Dysregulation of these metabolites is mainly reflected in the possible induction of hepatitis, oxidative stress, and other symptoms. The application of iEESI-MS technology without sample pretreatment to the study of metabolic disorders in aquatic organisms under the interference of nano-and microplastics provides a promising analytical method for environmental toxicology research.

show abstract

Section: Resultsmentioning

confidence: 99%

Liver Metabolic Dysregulation Induced by Polypropylene Nano- and Microplastics in Nile Tilapia Using Internal Extractive Electrospray Ionization Mass Spectrometry

Yang

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

show abstract

“…Another category was amino acids and their derivatives, which might affect the body's antioxidant capacity and immune response. 23 Meanwhile, several other disturbed metabolites were also involved. Acylcarnitines are associated with energy metabolism.…”

Section: Resultsmentioning

confidence: 99%

Machine learning encodes urine and serum metabolic patterns for autoimmune disease discrimination, classification and metabolic dysregulation analysis

Wang

Chen

et al. 2023

Analyst

View full text Add to dashboard Cite

show abstract

“…The accumulation of amino acids in the crucian carp kidney increased with an increase in carbonates, implying that increasing inhibition of protein synthesis in the crucian carp kidney was seen with increasing concentration of carbonate alkali. Environmental stress causes amino acid metabolism disorder in fish, which in turn interferes with aminoacyl-tRNA biosynthesis and protein digestion and absorption, which has been found in olive flounder ( Paralichthys olivaceus ) [ 40 ], swordtail fish ( Xiphophorus helleri ) [ 50 ], and common carp ( Cyprinus carpio ) [ 51 ]. Collectively, based on the analysis above, crucian carp could suppress protein and amino acid catabolism of the kidney to reduce endogenous ammonia production for the adaptations of the saline-alkaline environment.…”

Section: Discussionmentioning

confidence: 99%

Effects of Saline-Alkaline Stress on Metabolome, Biochemical Parameters, and Histopathology in the Kidney of Crucian Carp (Carassius auratus)

et al. 2023

View full text Add to dashboard Cite

The salinization of the water environment caused by human activities and global warming has increased which has brought great survival challenges to aquatic animals. Crucian carp (Carassius auratus) is an essential freshwater economic fish with superior adaptability to saline-alkali water. However, the physiological regulation mechanism of crucian carp adapting to saline-alkali stress remains still unclear. In this study, crucian carp were exposed to freshwater or 20, 40, and 60 mmol/L NaHCO3 water environments for 30 days, the effects of saline-alkali stress on the kidney were evaluated by histopathology, biochemical assays and metabolomics analysis from renal function, antioxidant capacity and metabolites level. Our results showed different degrees of kidney damage at different exposure concentrations, which were characterized by glomerular atrophy and swelling, renal tubular degranulation, obstruction and degeneration, renal interstitial edema, renal cell proliferation and necrosis. Saline-alkali stress could change the levels of several physiological parameters with renal function and antioxidant capacity, including creatinine (CREA), urea nitrogen (BUN), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). In addition, metabolomics analysis showed that differential metabolites (DMs) were involved in various metabolic pathways, including phenylalanine, tyrosine, and tryptophan biosynthesis, aminoacyl-tRNA biosynthesis, purine metabolism, glycerophospholipid metabolism, sphingolipid metabolism, glycolysis/gluconeogenesis and the TCA cycle. In general, our study revealed that saline-alkaline stress could cause significant changes in renal function and metabolic profiles, and induce severe damage in the crucian carp kidney through destroying the anti-oxidant system and energy homeostasis, inhibiting protein and amino acid catabolism, as well as disordering purine metabolism and lipid metabolism. This study could contribute to a deeper understanding the adverse effects of saline-alkali stress on crucian carp kidney and the regulatory mechanism in the crucian carp of saline-alkali adaptation at the metabolic level.

show abstract

Effects of polystyrene microplastics acute exposure in the liver of swordtail fish (Xiphophorus helleri) revealed by LC-MS metabolomics

Cited by 24 publications

References 58 publications

Liver Metabolic Dysregulation Induced by Polypropylene Nano- and Microplastics in Nile Tilapia Using Internal Extractive Electrospray Ionization Mass Spectrometry

Liver Metabolic Dysregulation Induced by Polypropylene Nano- and Microplastics in Nile Tilapia Using Internal Extractive Electrospray Ionization Mass Spectrometry

Machine learning encodes urine and serum metabolic patterns for autoimmune disease discrimination, classification and metabolic dysregulation analysis

Effects of Saline-Alkaline Stress on Metabolome, Biochemical Parameters, and Histopathology in the Kidney of Crucian Carp (Carassius auratus)

Contact Info

Product

Resources

About