Effects of polyamines on the thermal stability and formation kinetics of DNA duplexes with abnormal structure

Hou, Ming-Hon; Lin, Shwu‐Bin; Yuann, Jeu-Ming P.; Lin, Wei Chen; Wang, Andrew H.-J.; Kan, Lou‐Sing

doi:10.1093/nar/29.24.5121

Cited by 93 publications

(78 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CD spectrum of pure DNA (Fig. 4, line a) shows two positive bands at 275 nm (large band) and 221 nm (weak band) and a negative one at 245 nm, typical of B-DNA conformation (75,76). The DNA bands at 275 and 245 nm were not changed upon the addition of cobalt(II-I)hexamine suggesting that DNA remain as B-conformation.…”

Section: Polyamines-dna Complexes Studied By Ftir Spectroscopy-mentioning

confidence: 99%

Structural Analysis of DNA Interactions with Biogenic Polyamines and Cobalt(III)hexamine Studied by Fourier Transform Infrared and Capillary Electrophoresis

Ouameur

Tajmir-Riahi

2004

Journal of Biological Chemistry

284

234

View full text Add to dashboard Cite

Biogenic polyamines, such as putrescine, spermidine, and spermine are small organic polycations involved in numerous diverse biological processes. These compounds play an important role in nucleic acid function due to their binding to DNA and RNA. It has been shown that biogenic polyamines cause DNA condensation and aggregation similar to that of inorganic cobalt(III)hexamine cation, which has the ability to induce DNA conformational changes. However, the nature of the polyamine⅐DNA binding at the molecular level is not clearly established and is the subject of much controversy. In the present study the effects of spermine, spermidine, putrescine, and cobalt(III)hexamine on the solution structure of calf-thymus DNA were investigated using affinity capillary electrophoresis, Fourier transform infrared, and circular dichroism spectroscopic methods. At low polycation concentrations, putrescine binds preferentially through the minor and major grooves of double strand DNA, whereas spermine, spermidine, and cobalt(III)hexamine bind to the major groove. At high polycation concentrations, putrescine interaction with the bases is weak, whereas strong base binding occurred for spermidine in the major and minor grooves of DNA duplex. However, major groove binding is preferred by spermine and cobalt(III)hexamine cations. Electrostatic attractions between polycation and the backbone phosphate group were also observed. No major alterations of B-DNA were observed for biogenic polyamines, whereas cobalt(III)hexamine induced a partial B 3 A transition. DNA condensation was also observed for cobalt(III)hexamine cation, whereas organic polyamines induced duplex stabilization. The binding constants calculated for biogenic polyamines are K Spm ‫؍‬ 2.3 ؋ 10 5 M ؊1 , K Spd ‫؍‬ 1.4 ؋ 10 5 M ؊1 , and K Put ‫؍‬ 1.02 ؋ 10 5 M ؊1 . Two binding constants have been found for cobalt(III)hexamine with K 1 ‫؍‬ 1.8 ؋ 10 5 M ؊1 and K 2 ‫؍‬ 9.2 ؋ 10 4 M ؊1 . The Hill coefficients indicate a positive cooperativity binding for biogenic polyamines and a negative cooperativity for cobalt(III)hexamine.The biogenic polyamines putrescine [NH 2 (CH 2 ) 4 NH 2 ], spermidine [NH 2 (CH 2 ) 4 NH(CH 2 ) 3 NH 2 ], and spermine [NH 2 (C-H 2 ) 3 NH(CH 2 ) 4 NH(CH 2 ) 3 NH 2 ] (Structure 1) are the most prevalent polyamines in mammalian cells. They are small aliphatic polycations involved in numerous diverse biological processes, such as the ability to modulate gene expression and enzyme activities, activation of DNA synthesis, cell proliferation and differentiation, and others (1-8). Other functions of polyamines have been related to DNA protection from external agents (9, 10) and against radiation damage (11, 12). In general, spermidine and spermine are present in millimolar concentrations in vivo, whereas putrescine levels are slightly lower (13-15). However, the most important characteristic of polyamines within the cells is to bind nucleic acids and DNA in particular (1, 6).Polyamines also have the ability to induce DNA conformational transitions. Their bindi...

show abstract

Section: Polyamines-dna Complexes Studied By Ftir Spectroscopy-mentioning

confidence: 99%

Structural Analysis of DNA Interactions with Biogenic Polyamines and Cobalt(III)hexamine Studied by Fourier Transform Infrared and Capillary Electrophoresis

Ouameur

Tajmir-Riahi

2004

Journal of Biological Chemistry

284

234

View full text Add to dashboard Cite

show abstract

“…Multivalent cations such as spermine and spermidine are known to associate and stabilize DNA oligonucleotides [13,14] and to play an important role in packaging. Both spermine and spermidine bind to DNA nonselectively and occupy sites along the backbone due to strong electrostatic association bridging both the major and minor grooves [15].…”

Section: Protecting Dna From Oxidative Damagementioning

confidence: 99%

Oxidative damage to DNA: Inhibition of guanine damage

Kanvah

Schuster

2006

Pure and Applied Chemistry

View full text Add to dashboard Cite

One-electron oxidation of DNA results in chemical damage to nucleobases, particularly guanine in multiple G sequences. Oxidation may be triggered by numerous events, including photosensitization. We describe studies of photoinduced oxidations of DNA triggered by irradiation of covalently linked anthraquinone derivatives under various conditions that affect the global structure of the DNA. These structural changes have subtle effects on the result of the one-electron oxidation.

show abstract

“…18,20) Polyamines bound to nucleic acids can induce aggregation, structural changes of DNA and can stabilize DNA in vitro. [21][22][23][24][25] Based on these insights, SPM and polyamine analogs such as poly (L-lysine), polyethylenimine, etc., have been examined for the construction of nanoparticles containing small interfering RNA (siRNA) or plasmid DNA for delivering genes to specific target organs or cultured cells. [26][27][28][29][30][31] Interestingly, poly-ion complex (PIC) formation through the electrostatic interaction between polylysine and CS, has been reported.…”

Section: 3)mentioning

confidence: 99%

Poly-ion Complex of Chondroitin Sulfate and Spermine and Its Effect on Oral Chondroitin Sulfate Bioavailability

Higashi

Ito

Kenichi

et al. 2016

CHEMICAL & PHARMACEUTICAL BULLETIN

View full text Add to dashboard Cite

Chondroitin sulfate (CS) has been accepted as an ingredient in health foods for the treatment of symptoms related to arthritis and cartilage repair. However, CS is poorly absorbed through the gastrointestinal tract because of its high negative electric charges and molecular weight (MW). In this study, poly-ion complex (PIC) formation was found in aqueous solutions through electrostatic interaction between CS and polyamines-organic molecules having two or more primary amino groups ubiquitously distributed in natural products at high concentrations. Characteristic properties of various PICs generated by mixing CS and natural polyamines, including unusual polyamines, were studied based on the turbidity for PIC formation, the dynamic light scattering for the size of PIC particles, and ζ-potential measurements for the surface charges of PIC particles. The efficiency of PIC formation between CS and spermine increased in a CS MW-dependent manner, with 15 kDa CS being critical for the formation of PIC (particle size: 3.41 µm) having nearly neutral surface charge (ζ-potential: 0.80 mV). Comparatively, mixing tetrakis(3-aminopropyl)ammonium and 15 kDa of CS afforded significant levels of PIC (particle size: 0.42 0.16 µm) despite a strongly negative surface charge ( 34.67 1.15 mV). Interestingly, the oral absorption efficiency of CS was greatly improved only when PIC possessing neutral surface charges was administered to mice. High formation efficiency and electrically neutral surface charge of PIC particles are important factors for oral CS bioavailability.

show abstract

Effects of polyamines on the thermal stability and formation kinetics of DNA duplexes with abnormal structure

Cited by 93 publications

References 45 publications

Structural Analysis of DNA Interactions with Biogenic Polyamines and Cobalt(III)hexamine Studied by Fourier Transform Infrared and Capillary Electrophoresis

Structural Analysis of DNA Interactions with Biogenic Polyamines and Cobalt(III)hexamine Studied by Fourier Transform Infrared and Capillary Electrophoresis

Oxidative damage to DNA: Inhibition of guanine damage

Poly-ion Complex of Chondroitin Sulfate and Spermine and Its Effect on Oral Chondroitin Sulfate Bioavailability

Contact Info

Product

Resources

About