Effects of Poly-γ-glutamic Acid on Calcium Absorption in Rats

Yang, Li-Chan; Wu, Jin‐Bin; Ho, Guan-Huei; Yang, Shih-Ching; Huang, Yun-Pen; Lin, Wen‐Chuan

doi:10.1271/bbb.80024

Cited by 22 publications

(16 citation statements)

References 12 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The remaining ash was weighed, solubilized with 0.1Mol/L HCI, transferred into volumetric flask and completed to 100 ml with 0.1Mol/L HCI according to Yang et al (2008). The final solution was used for estimation of calcium and phosphorus in the ash using colorimetric methods.…”

Section: Bone Analysismentioning

confidence: 99%

Effect of diets fortified with Nigella sativa, sesame seeds and high calcium on osteoporosis in ovariectomized rats

Ramadan

2014

Egyptian Journal of Nutrition and Health

View full text Add to dashboard Cite

The study was carried out to assess the effect of diets fortified with Nigella sativa (NS), sesame (SS) seeds and high calcium (Ca) on osteoporosis in ovariectomized (OVX) rats. Fifty six rats were randomized into 8 equal groups (n= 7) and housed individually in metal metabolic cages. Under ether anesthesia, group (1) was SHAM-operated and fed on basal diet, while the other 7 groups were bilaterally OVX-operated. Three weeks after ovariectomy, group (2) was fed on basal diet, while groups (3), (4), (5), (6) and (7) were fed on experimental diets fortified with the following: 10% NS; 10% SS; 2% Ca; 10% NS+2% Ca and 10% SS+2°/0 Ca, respectively for 6 weeks. Group (8) was fed on basal diet and given orally Alendronate (anti-osteoporotic drug) in a daily dose of 3 mg/kg for 6 weeks. During feeding period, initial and final body weights of rats were recorded. At the end of the experiment, the last 24 hr urine samples were collected for estimating Ca, P, pyridinoline (PD) and deoxypyridinoline (DPD) concentrations. Rats were then euthanized by prolonged exposure to anesthetic ether. Blood samples were collected for estimating serum calcium (Ca), phosphorous (P), bone-specific alkaline phosphatase (b-ALP) and osteocalcin (OC) levels. The uteri were dissected out and weighed.Femur bones also were dissected out and used for measurement and analysis of bone ash. The results showed that diets fortified with NS and SS and high calcium prevented the increase in body weight gain and the decrease in uterine weight induced by ovariectomy. These experimental diets normalized serum Ca, P, b-ALP and OC levels and decreased urinary excretion of Ca, P, PD and DPD. There were also significant increases in femur bone mineral density (BMD) and calcium content in bone ash. In conclusion, diets fortified with NS, SS and high calcium have a promising antiosteoporotic effect in OVX rats. The study recommends enrichment of the bakeries and food stuff with Nigella sativa and sesame seeds and eating meals rich in calcium such as dairy products (milk and yoghurt) may be beneficial for the treatment of postmenopausal osteoporosis in women. Biochemical analysis; Bone mineralization. Agata, U.; Park, J.H.; Hattori, S.; Limura, Y.; Ezawa, I.; Akimoto, T. and Omi, N. (2013): The effect of different amounts of calcium intake on bone metabolism and arterial calcification in ovariectomized rats. J. Nutri. Sci. Vitaminol.; 59(1): 29-36. Ahmad, S. and Beg, Z.H. (2013): Hypolipidemic and antioxidant activities of Thymoquinone and limonene in atherogenic suspension fed rats. Food Chem.; 2013 Jun 1; 138(3):S. (2011): Preventive effects of Phytoestrogens against postmenopausal osteoporosis as compared to the available therapeutic choices: an overview. J. Nat. Sci. Biol. Med.; 2(2):154-163. Al-Majed, A.A.; Al-Omar, F.A. and Nagi, M.N. (2006): Neuroprotective effects of Thymoquinone against transient forebrain ischemia in the rat hippocampus. Europ. J. Pharmacol.; 543 (3):40-47. Atlan, M.F. (2007): Effects of Nigella sativa and human parathyroid hormone on bon...

show abstract

Section: Bone Analysismentioning

confidence: 99%

Effect of diets fortified with Nigella sativa, sesame seeds and high calcium on osteoporosis in ovariectomized rats

Ramadan

2014

Egyptian Journal of Nutrition and Health

View full text Add to dashboard Cite

show abstract

“…Therefore, g-PGA is considered to increase nonsaturable transport, especially in the lower intestine, which is consistent with the data that g-PGA increases Ca solubility in the lower small intestine (Tanimoto et al 2001a;Park et al 2005). However, it is reported that g-PGA decreased Ca solubility when apparent Ca absorption and retention was accelerated by g-PGA (Yang et al 2008). Furthermore, expression of calbinding-D9k, a vitamin D-induced Ca-binding protein, in the upper small intestine was also increased in the same experiment, which suggested that g-PGA increased saturable Ca transport in the upper small intestine.…”

Section: Enhancing Intestinal Calcium Absorptionmentioning

confidence: 89%

“…They reported Ca solubilizing ability in the mouse small intestine of high-molecular-weight g-PGA (MW 1,000 K) was higher than that of g-PGA (MW 500 K) and suggested that molecular size of g-PGA was correlated with Ca solubility. Moreover, Ca solubilizing ability of g-PGA in vitro was also reported (Yang et al 2008). They conducted single oral dose study with rats and investigated intestinal Ca solubility and Ca absorption assessed by plasma Ca kinetics.…”

Section: Enhancing Intestinal Calcium Absorptionmentioning

confidence: 99%

Food Applications of Poly-Gamma-Glutamic Acid

Tanimoto

2010

Amino-Acid Homopolymers Occurring in Nature

View full text Add to dashboard Cite

“…For example, poly-c-glutamic acid is generated during soybean fermentation 34 and has shown great potential as a drug delivery platform. 35 Moreover, other substances generated during soybean fermentation have also been reported to increase the calcium content of bones and prevent osteoporosis 25,36 and, thus, may have further augmented MK-7 to enhance the preventive efficacy on bone loss. Further studies are needed to define the association between these bioactive materials and MK-7.…”

Section: Discussionmentioning

confidence: 99%

The Antiosteoporotic Effects of Cheonggukjang Containing Vitamin K₂ (Menaquinone-7) in Ovariectomized Rats

Lee

et al. 2014

Journal of Medicinal Food

View full text Add to dashboard Cite

The effect of dietary vitamin K2 (menaquinone-7, MK-7) and cheonggukjang (CGJ) on the prevention of ovariectomy (OVX)-induced bone loss was studied in rats. Female Sprague-Dawley rats were divided into eight groups: sham-operated; OVX control; OVX treated with MK-7 at doses of 2, 4, and 8 μg/day; and OVX treated with CGJ at doses of 0.063, 0.125, and 0.250 g/day referenced to MK-7 levels at 2, 4, and 8 μg/day, respectively. After 8 weeks of treatment, the preventive effects of MK-7 and CGJ were evaluated by measuring body weights, serum levels of bone turnover markers, bone mineral content (BMC), bone mineral density (BMD), trabecular microarchitectural properties, and bone histological characteristics. Our results showed that rats treated with a high dose of MK-7 (8 μg/day) exhibited a minor inhibitory effect on OVX-induced bone loss, as indicated by a significant increase in trabecular number, as well as BMC and BMD (P<.01). Moreover, the preventive effects of MK-7 were augmented by administration of CGJ at the same MK-7 dose. In addition, the preventive effects of CGJ were shown to be dose dependent, with the highest dose (0.250 g/day) significantly (P<.01) increasing BMC and BMD by 31.8% and 47.6%, respectively. In summary, these results suggest that administration of CGJ containing abundant levels of MK-7 may be a promising approach for the treatment and prevention of osteoporosis.

show abstract

Effects of Poly-γ-glutamic Acid on Calcium Absorption in Rats

Cited by 22 publications

References 12 publications

Effect of diets fortified with Nigella sativa, sesame seeds and high calcium on osteoporosis in ovariectomized rats

Effect of diets fortified with Nigella sativa, sesame seeds and high calcium on osteoporosis in ovariectomized rats

Food Applications of Poly-Gamma-Glutamic Acid

The Antiosteoporotic Effects of Cheonggukjang Containing Vitamin K₂ (Menaquinone-7) in Ovariectomized Rats

Contact Info

Product

Resources

About

Effects of Poly-γ-glutamic Acid on Calcium Absorption in Rats

Cited by 22 publications

References 12 publications

Effect of diets fortified with Nigella sativa, sesame seeds and high calcium on osteoporosis in ovariectomized rats

Effect of diets fortified with Nigella sativa, sesame seeds and high calcium on osteoporosis in ovariectomized rats

Food Applications of Poly-Gamma-Glutamic Acid

The Antiosteoporotic Effects of Cheonggukjang Containing Vitamin K2 (Menaquinone-7) in Ovariectomized Rats

Contact Info

Product

Resources

About

The Antiosteoporotic Effects of Cheonggukjang Containing Vitamin K₂ (Menaquinone-7) in Ovariectomized Rats