Effects of PM2.5 on mucus secretion and tissue remodeling in a rabbit model of chronic rhinosinusitis

Zhao, Ru; Dong, Weiyang; Deng, Congrui; Han, Zhijin; Liu, Jian; Wang, Hanwei; Zhuang, Guoshun; Zhang, Ruxin

doi:10.1002/alr.22182

Cited by 29 publications

(29 citation statements)

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“…PM2.5 was prepared as previously described. [3][4][5]12 Briefly, PM2.5 was collected from the roof (at a height of approximately 20 m) of a 5-story building located on the campus of Fudan University (31.3 N, 121.5 E), Shanghai, China. The PM was collected using Whatman 41 filters (Whatman, Maidstone, UK) with TSP/PM10/PM2.5-2 samplers (Dickel, Beijing, China) at a flow rate of 77.59 L/min.…”

Section: Pm25 Sample Preparation and Chemical Analysismentioning

confidence: 99%

Effects of Ursolic Acid on the Expression of Th1–Th2-related Cytokines in a Rat Model of Allergic Rhinitis After PM2.5 Exposure

Sun

Han

Wang

et al. 2020

Am J Rhinol�Allergy

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Background Allergic rhinitis (AR) is a global health problem and closely related to environmental factors. Ursolic acid (UA) has potential in the treatment of allergic inflammation. The effects of UA intervention on PM2.5-induced AR remain uncertain. Objective To assess the effects of UA on nasal symptoms and the expression of T-helper (Th)1–Th2-related cytokines in a rat model of AR after fine particulate matter (particulate matter ≤ 2.5 µm [PM2.5]) exposure. Methods A total of 40 healthy female Sprague-Dawley rats were randomly divided into 4 groups: normal control group (NC group), ovalbumin (OVA)- induced AR model (AR group), PM2.5-exposed AR group exposed to 200 g/m3 PM2.5 for 30 days via inhalation (ARE group), and a group with UA intervention to the AR model after PM2.5 exposure (UA group). UA intervention was adopted after PM2.5 exposure in the UA group. Nasal symptoms and levels of Th1–Th2 cytokines in the serum were detected in each individual rat. The pathological changes and expression of Eotaxin in the nasal mucosa of each individual rat were examined by histology. Results PM2.5 significantly increased the number of sneezes and nasal rubs in the rats with AR, and UA alleviated these symptoms. UA decreased interleukin (IL)-4, IL-5, IL-13, Eotaxin-1, and OVA Immunoglobulin E (IgE) protein levels. In the AR group, hematoxylin and eosin staining showed disordered arrangement of the nasal mucosa epithelium, cell shedding, eosinophilic infiltration, swelling of the glands, and submucosal vascular congestion. UA group showed reduced eosinophilic infiltration and orderly arrangement of the mucosal epithelium when compared with the ARE group. Immunohistochemical results showed that the expression of Eotaxin in the UA group was lower than that in the ARE group. Conclusion UA could relieve nasal symptoms caused by PM2.5 exposure, the possible mechanism of which is to inhibit the expression of Th2 cytokines, eosinophilic infiltration, and specific IgE production.

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Section: Pm25 Sample Preparation and Chemical Analysismentioning

confidence: 99%

Effects of Ursolic Acid on the Expression of Th1–Th2-related Cytokines in a Rat Model of Allergic Rhinitis After PM2.5 Exposure

Sun

Han

Wang

et al. 2020

Am J Rhinol�Allergy

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“…Five representative and non-overlapping fields were chosen and counted under 400×magnification, as previously described. 16 In each chosen area, the hyperplasia of the goblet cells in the epithelial layer was determined by a score according to the percentage of the goblet cells in the epithelial cells described below. A five-point scoring system (grades 0–4) was adopted, as previously performed 16 : grade 0, no goblet cells; grade 1, <25%; grade 2, 25–50%; grade 3, 51–75%; and grade 4, >75%.…”

Section: Methodsmentioning

confidence: 99%

“…PM2.5 sample was collected according to previously described methods. [5][6][7]14,16 Briefly, the fine particulate matter was collected from the roof of a five-story building (approximately 20 m above ground) on the campus of Fudan University (31.3 N, 121.5 E), in Shanghai, China. The particulate matter was collected on Whatman 41 filters (Whatman, Maidstone, U.K.) using TSP/PM10/PM2.5-2 samplers (Dickel, Beijing, China) with a 77.59 L/min flow rate.…”

Section: Pm25 Sample Preparation and Chemical Analysismentioning

confidence: 99%

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Ursolic Acid Alleviates Mucus Secretion and Tissue Remodeling in Rat Model of Allergic Rhinitis After PM2.5 Exposure

Sun

Deng

Zhao

et al. 2020

Am J Rhinol�Allergy

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Background According to recent epidemiologic studies, exposure to fine particulate matter (particulate matter 2.5 ≤ µm [PM2.5]) in the air increases the incidence and severity of allergic rhinitis (AR). Ursolic acid (UA) has activities in immune regulation and anti-inflammatory. However, the role of UA intervention on PM2.5-exposed AR remains unknown. In this study, we investigated the effects of UA on tissue remodeling and mucus hypersecretion in a rat model of AR after PM2.5 exposure. Methods AR was induced in rats with ovalbumin (OVA) and they were exposed to ambient PM2.5(200 µg/m3) via a PM2.5 inhalation exposure system for 30 days(ARE group). Ursolic acid intervention was administrated in the AR model after PM2.5 exposure (UA group). Hyperplasia of goblet cells was detected by periodic acid-Schiff (PAS) staining and collagen deposition in the nasal mucosa was detected by Masson trichrome (MT) staining.MUC5AC expression was measured by immunohistochemistry. Results UA group showed reduced goblet cell hyperplasia and collagen deposition in the nasal mucosa which exacerbated after PM2.5 exposure, as reflected by PAS and MT staining when compared with the ARE group. Immunohistochemical results showed that the expression of MUC5AC in the UA group was lower than that in the ARE group. Conclusion Analysis of our data indicated that UA could attenuate nasal remodeling and mucus hypersecretion in aggravation of AR after PM2.5 exposure, which may be the pathophysiologic mechanisms for the prevention of AR exacerbated by exposure to PM2.5.

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“…In order to elucidate the role of particularly particulate air pollution in CRS, Zhao et al . established a rabbit model of CRS exposed to fine particulate matter (particulate matter ≤2.5 μm [PM2.5]).…”

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Rising to the challenges of precision medicine: nasal inflammation research in China

Zhang

2018

Int Forum Allergy Rhinol

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Chronic rhinosinusitis (CRS) and allergic rhinitis (AR) have exhibited increasing prevalence in China. Although 1 longitudinal study demonstrated that the prevalence of selfreported adult AR in several major cities in mainland China increased markedly from 11.1% to 17.6% during a 6-year period, 1 a more recent epidemiological study reported a prevalence of 32.4% physician-diagnosed AR in the grasslands of northern China. 2 Similarly, a cross-sectional survey of CRS in several cities in China reported CRS to be up to 8% (range, 4.8% to 9.7%). 3 Considering the huge population base as well as the great number of patients, the number of physicians currently registered as Ear, Nose, and Throat (ENT) specialists is less than 38,000, with only a small portion being professional rhinologists. Based on the high prevalence and the consequential socioeconomic burden, more studies on the pathogenesis, phenotyping and endotyping, and efficacy of variable treatment options in CRS and AR are needed in China. Over the past decade, increased national financial support has enabled increased numbers of investigations in the field of sinonasal research, leading to significant progress and developments. Thus, this issue of the International Forum of Allergy and Rhinology (IFAR) highlights these developments, and gives the reader a broad and succinct overview of this progressively evolving field.We are entering a new era of precision medicine in rhinology; recent studies, especially in Chinese patients with chronic rhinosinusitis with nasal polyps (CRSwNP), have substantially expanded our understanding of the pathophysiologic and immunologic characteristics of CRS. First, these studies have shown that the inflammatory patterns of CRS are not universal worldwide. Studies have demonstrated that although a pronounced eosinophilic infiltration is predominant in Western patients with nasal polyps, 4 the eosinophilic phenotype constitutes less than one-half of the cases of CRSwNP in China. 5 Thus, the "one size fits all" strategy is no longer suitable in the clinical practice of CRS. Furthermore, these studies have indicated that due to the existence of geographic, ethnic, and environmental differences, both location-specific and ethnic-specific

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Effects of PM2.5 on mucus secretion and tissue remodeling in a rabbit model of chronic rhinosinusitis

Abstract: Increases in ciliary disruption, nasal remodeling, and mucus hypersecretion may be the pathophysiologic mechanisms underlying the PM2.5-induced aggravation of CRS.

Cited by 29 publications

References 29 publications

Effects of Ursolic Acid on the Expression of Th1–Th2-related Cytokines in a Rat Model of Allergic Rhinitis After PM2.5 Exposure

Effects of Ursolic Acid on the Expression of Th1–Th2-related Cytokines in a Rat Model of Allergic Rhinitis After PM2.5 Exposure

Ursolic Acid Alleviates Mucus Secretion and Tissue Remodeling in Rat Model of Allergic Rhinitis After PM2.5 Exposure

Rising to the challenges of precision medicine: nasal inflammation research in China

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