“…The total nitrogen content of the matrix was determined using Automatic Kjeldahl Apparatus(KN520, Jinan Alva Instrument Co., Ltd. is located in Jinan City, Shandong Province, China), while the total phosphorus content of the matrix was measured using the phosphomolybdate colourimetric method [30]. The total potassium content of the matrix was determined using flame atomic absorption spectroscopy [31].…”
Section: Determination Of Experimental Indexesmentioning
confidence: 99%
“…The total potassium content of the matrix was determined using flame atomic absorption spectroscopy [31]. The organic matter content of the matrix was determined using the potassium dichromate volumetric method [30]. Each treatment was replicated three times to ensure statistical validity.…”
Section: Determination Of Experimental Indexesmentioning
(1) Background: Low-calcium stress can have adverse effects on the rhizosphere environment of cucumber, subsequently impacting cucumber growth. However, plant-growth-promoting rhizobacteria can directly or indirectly enhance plant growth and induce plant tolerance, thereby mitigating the detrimental effects of low-calcium stress on cucumber growth. This study aims to elucidate the role of Bacillus amyloliquefaciens QST713 in the rhizosphere environment of cucumber under low-calcium stress, providing a theoretical basis for the application and promotion of Bacillus amyloliquefaciens. (2) Methods: This study used the ‘JinYou NO.4′ cucumber variety as test material, setting four treatments of CK, CK+Q, LCa, LCa+Q. We conducted measurements of plant height and stem diameter for four groups of cucumber plants: before treatment (0 d), and at 10 d, 20 d, 30 d, and 60 d after treatment. Additionally, we determined the biomass of cucumber plants under different treatments during the peak fruiting period. Inter-root matrix samples of cucumber were collected during the fruiting late period, and the physical and chemical properties and nutrient contents of the inter-root matrix of cucumber were determined, and bacterial microbial diversity and bacterial microbial communities were analysed using Illumina-MiSeq high-throughput sequencing technology. (3) Results: Low-calcium stress significantly inhibits the growth of cucumber plants. However, the application of Bacillus amyloliquefaciens QST713 effectively mitigates the inhibitory effects of low-calcium stress on cucumber growth. The application of Bacillus amyloliquefaciens QST713 was able to improve the physicochemical environment of the matrix and enhanced the absorption and utilisation of matrix nutrients in cucumber. The high-throughput sequencing analysis showed that the richness and diversity of bacterial communities and the number of bacteria decreased significantly under low-calcium stress, and increased significantly after the application of Bacillus amyloliquefaciens QST713. The composition of the dominant bacterial groups of the inter-root matrix of cucumber was basically the same among the four treatments, and the main difference was in the abundance of bacteria. The application of Bacillus amyloliquefaciens QST713 increased the relative abundance of bacteria that decreased under low-calcium stress, and decreased the relative abundance of bacteria that increased under low-calcium stress. (4) Conclusions: The results of this study elucidated the positive effects of Bacillus amyloliquefaciens QST713 on the growth and inter-root environment of cucumber under low-calcium stress, and provided a theoretical basis for in-depth research on the resistance of Bacillus amyloliquefaciens and its popularised application.
“…The total nitrogen content of the matrix was determined using Automatic Kjeldahl Apparatus(KN520, Jinan Alva Instrument Co., Ltd. is located in Jinan City, Shandong Province, China), while the total phosphorus content of the matrix was measured using the phosphomolybdate colourimetric method [30]. The total potassium content of the matrix was determined using flame atomic absorption spectroscopy [31].…”
Section: Determination Of Experimental Indexesmentioning
confidence: 99%
“…The total potassium content of the matrix was determined using flame atomic absorption spectroscopy [31]. The organic matter content of the matrix was determined using the potassium dichromate volumetric method [30]. Each treatment was replicated three times to ensure statistical validity.…”
Section: Determination Of Experimental Indexesmentioning
(1) Background: Low-calcium stress can have adverse effects on the rhizosphere environment of cucumber, subsequently impacting cucumber growth. However, plant-growth-promoting rhizobacteria can directly or indirectly enhance plant growth and induce plant tolerance, thereby mitigating the detrimental effects of low-calcium stress on cucumber growth. This study aims to elucidate the role of Bacillus amyloliquefaciens QST713 in the rhizosphere environment of cucumber under low-calcium stress, providing a theoretical basis for the application and promotion of Bacillus amyloliquefaciens. (2) Methods: This study used the ‘JinYou NO.4′ cucumber variety as test material, setting four treatments of CK, CK+Q, LCa, LCa+Q. We conducted measurements of plant height and stem diameter for four groups of cucumber plants: before treatment (0 d), and at 10 d, 20 d, 30 d, and 60 d after treatment. Additionally, we determined the biomass of cucumber plants under different treatments during the peak fruiting period. Inter-root matrix samples of cucumber were collected during the fruiting late period, and the physical and chemical properties and nutrient contents of the inter-root matrix of cucumber were determined, and bacterial microbial diversity and bacterial microbial communities were analysed using Illumina-MiSeq high-throughput sequencing technology. (3) Results: Low-calcium stress significantly inhibits the growth of cucumber plants. However, the application of Bacillus amyloliquefaciens QST713 effectively mitigates the inhibitory effects of low-calcium stress on cucumber growth. The application of Bacillus amyloliquefaciens QST713 was able to improve the physicochemical environment of the matrix and enhanced the absorption and utilisation of matrix nutrients in cucumber. The high-throughput sequencing analysis showed that the richness and diversity of bacterial communities and the number of bacteria decreased significantly under low-calcium stress, and increased significantly after the application of Bacillus amyloliquefaciens QST713. The composition of the dominant bacterial groups of the inter-root matrix of cucumber was basically the same among the four treatments, and the main difference was in the abundance of bacteria. The application of Bacillus amyloliquefaciens QST713 increased the relative abundance of bacteria that decreased under low-calcium stress, and decreased the relative abundance of bacteria that increased under low-calcium stress. (4) Conclusions: The results of this study elucidated the positive effects of Bacillus amyloliquefaciens QST713 on the growth and inter-root environment of cucumber under low-calcium stress, and provided a theoretical basis for in-depth research on the resistance of Bacillus amyloliquefaciens and its popularised application.
“…Currently, mechanized agricultural production has been extensively employed across China, not only to conserve water and improve land utilization but also to reduce the physical labor required of farmers and decrease production costs [2]. However, shallow mechanical tillage, inappropriate irrigation, and the excessive application of fertilizers have resulted in deeper plow bottoms [3,4], high soil bulk density and compaction, and low porosity and oxygen concentrations in plow bottoms [5]. There are issues with shallow tillage that can cause a significant reduction in the effective soil in the tillage layer and a tendency towards the deterioration of the physical properties of the tilled soil [6].…”
Winter wheat and summer maize rotation is the main cropping pattern in the North China Plain (NCP). There are still problems with farmers’ production modes, including shallow tillage layers, single application of chemical fertilizer causing plow bottom layer thickening and soil pH decrease. A two-factor location experiment was conducted to investigate the effects of different tillage and fertilization patterns on the soil physical properties, soil organic carbon (SOC), nitrogen-use efficiency, and crop yield of wheat–maize rotation systems during the years 2018–2020. The different treatments were deep tillage + organic fertilizer (DTF), shallow tillage + organic fertilizer (STF), no tillage + organic fertilizer (NTF), deep tillage + nitrogen fertilizer (DT), shallow tillage + nitrogen fertilizer (ST), and no tillage + nitrogen fertilizer (NT). The results showed that STF treatment could effectively improve the physical properties of soil and, SOC content, and increase both the crop yield and revenue of farmers. In the STF treatment, soil water content was highest in the 0–20 cm layer (2018), which was 4.89–11.31% higher than that of the other treatments; additional organic fertilizer application reduced soil bulk and increased the proportion of <0.25 mm aggregates; SOC and soil total nitrogen (TN) content were highest in the 20–40 cm layer, (15.82–32.63% and 28.57–42.86%, respectively). The total yield of wheat–maize rotation for both years was the highest under STF treatment. The annual economic benefits under this treatment were 42,182.26 and 42,254.54 CNY ha−1, which were 1.02–12.94% and 2.29–9.87% higher than those of the other treatments. Therefore, the suggested planting method in the NCP is tillage of over 20 cm and additional organic fertilizer.
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