Effects of platelet-rich plasma on intestinal wound healing in pigs

Fresno, Laura; Fondevila, Dolors; Bambo, Otilia Rafael; Chacaltana, Asiul; García, Félix; Andaluz, Anna

doi:10.1016/j.tvjl.2009.06.009

Cited by 50 publications

(68 citation statements)

References 25 publications

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“…In veterinary medicine, little clinical information exists about the application of platelet-rich plasma. It is mainly used for equine tendon repair (Georg et al, 2010) and its application has also been reported on intestinal wound healing in pigs (Fresno et al, 2010) and on a large cutaneous lesion in a dog (Kim et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Intramammary administration of platelet concentrate as an unconventional therapy in bovine mastitis: First clinical application

Consiglio

Spelta

Garlappi³

et al. 2014

Journal of Dairy Science

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Bovine udder infections induce a variety of changes in gene expression of different growth factors that may suggest their possible role in glandular tissue protection or repair processes. Growth factors and also chemokines and cytokines may act synergistically to increase the infiltration of neutrophils and macrophages to promote angiogenesis, fibroplasia, matrix deposition, and, ultimately, re-epithelialization. Considering the vast applications, typically in human medicine, of platelet concentrate (PC) and its ease of preparation, the aim of our study was to evaluate an alternative therapy to stimulate the regeneration of glandular tissue, administering a concentration in excess of the growth factors contained in the PC. In each one of the 3 farms examined in the trial, PC was prepared from donor cows in good health, free from infections, and with no records of medications administered during the previous 2 mo. The platelet produced in one farm was used only for treating the cows of the same farm in a heterologous way. A total of 229 mastitic quarters were divided in 3 groups: antibiotic group (treated with intramammary antibiotic), antibiotic and PC group (treated intramammarily with antibiotics in association with PC), and PC group (treated with intramammary PC alone). The diagnosis of mastitis was based on somatic cell count and bacteriological evaluation of the milk from the affected quarter. Platelet concentrate, alone or in association with antibiotic, was used for 3 consecutive days as an unconventional therapy in bovine acute and chronic mastitis. Our data show that the associated action of antibiotic and PC performed significantly better than the antibiotic alone, either for the recovery of the affected mammary quarters or for somatic cell count reduction. In the same way, the association antibiotic plus PC showed significantly fewer relapses compared with the antibiotic alone, either for acute or chronic mastitis. The treatment with only PC did not show statistically significant differences compared with both antibiotic alone or associated treatment for acute mastitis, and it was better than the use of only antibiotic for chronic mastitis. Our results show that PC alone may be useful for a quick resolution of the inflammatory response, playing a role in limiting the tissue damage to the mammary gland parenchyma and reducing the recurrence rates.

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Section: Introductionmentioning

confidence: 99%

Intramammary administration of platelet concentrate as an unconventional therapy in bovine mastitis: First clinical application

Consiglio

Spelta

Garlappi³

et al. 2014

Journal of Dairy Science

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“…PRP preparations have been widely used in the treatment of diabetic foot ulcers, chronic ulcers, coronary artery bypass graft-associated foot ulcers, surgical ulcers, and corneal surgeries (10)(11)(12)(13)(14)(15)(16). PRP applications extend beyond cutaneous wounds; healing properties of platelet growth factors have been evaluated in gastrointestinal wounds, as well (11)(12)(13)(14)(15).…”

Section: Discussionmentioning

confidence: 99%

“…PL was loaded 1:1 into a pharmaceutical sterile vehicle (Carbapol 1%, ethylenediamine tetra acetic acid 0.05%, sodium metabisulfite 0.1%, sodium chloride 0.9% in adequate deionized water, all the percentages as w/v %). The vehicle pH was adjusted to 7.0 by sodium hydroxide 4N (8)(9)(10)(11)(12)(13)(14)(15). For preparing placebo, the saline solution 0.9% (W/V) was replaced by PL.…”

Section: Platelet-rich Plasma Preparationmentioning

confidence: 99%

Topical Platelet Lysate Hydrogel in Patients With Anal Fissure

Jamshidzadeh¹,

Mardani²,

Ghahramani³

et al. 2014

Ann Colorectal Res

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Background: Anal fissure is a painful tear or crack in the distal part of anal canal. It usually involves the epithelium, but in the long term it may extend to the whole anal mucosa. Platelet Lysate (PL) hydrogel is a hemoderivitive pharmaceutical formulation that contains plenty of growth factors. These growth factors can induce tissue regeneration and revascularization as well as fibroblast proliferation. Objectives: This study aimed to evaluate the effectiveness of PL hydrogel in the treatment of the anal fissure or retardation of its development. Patients and Methods:The patients with chronic and acute anal fissure were randomly divided into groups of A and B. Group A received PL hydrogel and Rectol, while group B received placebo and Rectol. Then, they were followed and evaluated for two weeks. Results: Although it was anticipated that platelet growth factors would be effective in anal fissure healing process, no significant difference was observed between the control group and the case group after the treatment. Nevertheless, a significant difference was observed within the two groups after the treatment. Conclusions: PL hydrogel does not seem to have beneficial effects compared to the placebo or Rectol on the healing of anal fissure.

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“…Breaking strength is typically measured using a tensiometer [23,24] by applying increasing force in a longitudinal direction to anastomotic segments until disruption takes place [16]. In this study, the resected intestinal segments, with the sutures in place, were placed in a dynamometer (Haas-Automatyka, Bielsko-Biała, Poland) at a force of 200 newtons (N); the force was then increased at a rate of 10 mm/min.…”

Section: Breaking Strengthmentioning

confidence: 99%

“…In this study, the resected intestinal segments, with the sutures in place, were placed in a dynamometer (Haas-Automatyka, Bielsko-Biała, Poland) at a force of 200 newtons (N); the force was then increased at a rate of 10 mm/min. The peak force necessary to induce disruption was considered the BS Ϭ (MPa) [23], which was calculated by dividing the maximum force by the intestinal cross-section: Fmax (N)/S (mm 2 ).…”

Section: Breaking Strengthmentioning

confidence: 99%

Compression Anastomosis Clips Versus a Hand-Sewn Technique for Intestinal Anastomosis in Pigs

Kuśnierz¹,

Kajor²,

Żaworonkow³

et al. 2015

Adv Clin Exp Med

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Different types of sutures, staples and sutureless anastomoses have been used to create anastomoses. The ideal device should ensure good serosal apposition without requiring either transgression of the bowel wall or the presence of foreign material for an extended period of time [2]. Healing occurs when the surgical wound is clean and sharp, Anastomotic dehiscence and leaks are major problems in gastrointestinal surgery and result in increased morbidity and mortality, thus decreasing overall survival. Intestinal anastomosis healing depends on the operative technique, the underlying medical condition, medical treatment and other individual, often unknown, factors AbstractBackground. Anastomotic dehiscence and leaks are major problems in gastrointestinal surgery and result in increased morbidity and mortality. The ideal device to create anastomoses should ensure good serosal apposition without requiring either transgression of the bowel wall or the presence of foreign material for an extended period of time. Objectives. The aim of this experimental study was to evaluate the safety and efficacy of a new compression anastomosis clip (CAC) for jejunojejunostomies and ileocolostomies by comparing CAC anastomoses with hand-sewn (HS) anastomoses in pigs in terms of healing, breaking strength and the time to create anastomoses. Material and Methods. The 11 pigs in the study underwent side-to-side CAC and HS jejunojejunostomies and ileocolostomies, for a total of 88 anastomoses. The pigs were sacrificed on postoperative day 5 (5 pigs) or 7 (6 pigs). Macroscopic, histopathological and breaking-strength examinations were performed. The time to create the anastomoses was recorded. Results. Neither group had anastomotic complications such as leakage or obstruction. Macroscopic examination showed no statistically significant differences between the groups. In the CAC group, the healing process was characterized by a lesser inflammatory reaction (p < 0.05) and very thin scar tissue at the anastomotic line (less collagen deposition and better epithelial regeneration), while the HS group had a much thicker anastomotic line. The breaking strength was significantly greater in the CAC group compared with the HS group (p < 0.05). The anastomosis time was shorter in the CAC group than in the HS group (p < 0.01). Conclusions. Anastomosis using a CAC appears to be safe and less time-consuming than HS; it was also characterized by a good healing process with little inflammatory reaction and a high breaking strength compared with HS anastomosis (Adv Clin Exp Med 2015, 24, 6, 000-000).

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Effects of platelet-rich plasma on intestinal wound healing in pigs

Cited by 50 publications

References 25 publications

Intramammary administration of platelet concentrate as an unconventional therapy in bovine mastitis: First clinical application

Intramammary administration of platelet concentrate as an unconventional therapy in bovine mastitis: First clinical application

Topical Platelet Lysate Hydrogel in Patients With Anal Fissure

Compression Anastomosis Clips Versus a Hand-Sewn Technique for Intestinal Anastomosis in Pigs

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